LOCUS       sequence-190250-       17053 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  DNA-PKcs T3950A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17053)
  AUTHORS   Douglas P, Cui X, Block WD, Yu Y, Gupta S, Ding Q, Ye R, Morrice N, 
            Lees-Miller SP, Meek K
  TITLE     The DNA-dependent protein kinase catalytic subunit is phosphorylated
            in vivo on threonine 3950, a highly conserved amino acid in the 
            protein kinase domain.
  JOURNAL   Mol Cell Biol. 2007 Mar;27(5):1581-91. Epub 2006 Dec 11.
  PUBMED    17158925
REFERENCE   2  (bases 1 to 17053)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "2007-03"; volume: "27(5)"; pages: "1581-91. Epub 2006
            Dec 11"
COMMENT     Sequence Label: DNA-PKcs T3950D
FEATURES             Location/Qualifiers
     source          1..17053
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      25..480
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(112..131)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     322..343
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     606..628
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     620..637
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     621..637
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        798..1177
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1178..1381
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1332..1352
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    13874..14496
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(14073..14092)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(14520..14540)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        14525..14854
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      14705..14840
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(14892..14911)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(14893..14911)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(14925..14941)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14925..14941)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(14938..14960)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    14949..14965
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(14973..14979,14980..14997,14998..15003))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15018..15039
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(15156..15173)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(15327..15915)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15407..15426)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(16086..16877,16878..16946))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     16709..16728
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(16947..17051)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 catttccccg aaaagtgcca cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg
       61 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
      121 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
      181 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
      241 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
      301 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
      361 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
      421 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
      481 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
      541 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
      601 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgatct atacattgaa
      661 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta
      721 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      781 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg
      841 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      901 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      961 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     1021 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
     1081 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
     1141 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
     1201 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     1261 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
     1321 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
     1381 tcgtttagtg aaccgtcaga attcagatct ggtaccacgc gtatcgataa gcttgcatgc
     1441 ctgcaggtcg actctagagc ggccgcatgg cgggctccgg agccggtgtg cgttgctccc
     1501 tgctgcggct gcaggagacc ttgtccgctg cggaccgctg cggtgctgcc ctggccggtc
     1561 atcaactgat ccgcggcctg gggcaggaat gcgtcctgag cagcagcccc gcggtgctgg
     1621 cattacagac atctttagtt ttttccagag atttcggttt gcttgtattt gtccggaagt
     1681 cactcaacag tattgaattt cgtgaatgta gagaagaaat cctaaagttt ttatgtattt
     1741 tcttagaaaa aatgggccag aagatcgcac cttactctgt tgaaattaag aacacttgta
     1801 ccagtgttta tacaaaagat agagctgcta aatgtaaaat tccagccctg gaccttctta
     1861 ttaagttact tcagactttt agaagttcta gactcatgga tgaatttaaa attggagaat
     1921 tatttagtaa attctatgga gaacttgcat tgaaaaaaaa aataccagat acagttttag
     1981 aaaaagtata tgagctccta ggattattgg gtgaagttca tcctagtgag atgataaata
     2041 atgcagaaaa cctgttccgc gcttttctgg gtgaacttaa gacccagatg acatcagcag
     2101 taagagagcc caaactacct gttctggcag gatgtctgaa ggggttgtcc tcacttctgt
     2161 gcaacttcac taagtccatg gaagaagatc cccagacttc aagggagatt tttaattttg
     2221 tactaaaggc aattcgtcct cagattgatc tgaagagata tgctgtgccc tcagctggct
     2281 tgcgcctatt tgccctgcat gcatctcagt ttagcacctg ccttctggac aactacgtgt
     2341 ctctatttga agtcttgtta aagtggtgtg cccacacaaa tgtagaattg aaaaaagctg
     2401 cactttcagc cctggaatcc tttctgaaac aggtttctaa tatggtggcg aaaaatgcag
     2461 aaatgcataa aaataaactg cagtacttta tggagcagtt ttatggaatc atcagaaatg
     2521 tggattcgaa caacaaggag ttatctattg ctatccgtgg atatggactt tttgcaggac
     2581 cgtgcaaggt tataaacgca aaagatgttg acttcatgta cgttgagctc attcagcgct
     2641 gcaagcagat gttcctcacc cagacagaca ctggtgacga ccgtgtttat cagatgccaa
     2701 gcttcctcca gtctgttgca agcgtcttgc tgtaccttga cacagttcct gaggtgtata
     2761 ctccagttct ggagcacctc gtggtgatgc agatagacag tttcccacag tacagtccaa
     2821 aaatgcagct ggtgtgttgc agagccatag tgaaggtgtt cctagctttg gcagcaaaag
     2881 ggccagttct caggaattgc attagtactg tggtgcatca gggtttaatc agaatatgtt
     2941 ctaaaccagt ggtccttcca aagggccctg agtctgaatc tgaagaccac cgtgcttcag
     3001 gggaagtcag aactggcaaa tggaaggtgc ccacatacaa agactacgtg gatctcttca
     3061 gacatctcct gagctctgac cagatgatgg attctatttt agcagatgaa gcatttttct
     3121 ctgtgaattc ctccagtgaa agtctgaatc atttacttta tgatgaattt gtaaaatccg
     3181 ttttgaagat tgttgagaaa ttggatctta cacttgaaat acagactgtt ggggaacaag
     3241 agaatggaga tgaggcgcct ggtgtttgga tgatcccaac ttcagatcca gcggctaact
     3301 tgcatccagc taaacctaaa gatttttcgg ctttcattaa cctggtggaa ttttgcagag
     3361 agattctccc tgagaaacaa gcagaatttt ttgaaccatg ggtgtactca ttttcatatg
     3421 aattaatttt gcaatctaca aggttgcccc tcatcagtgg tttctacaaa ttgctttcta
     3481 ttacagtaag aaatgccaag aaaataaaat atttcgaggg agttagtcca aagagtctga
     3541 aacactctcc tgaagaccca gaaaagtatt cttgctttgc tttatttgtg aaatttggca
     3601 aagaggtggc agttaaaatg aagcagtaca aagatgaact tttggcctct tgtttgacct
     3661 ttcttctgtc cttgccacac aacatcattg aactcgatgt tagagcctac gttcctgcac
     3721 tgcagatggc tttcaaactg ggcctgagct ataccccctt ggcagaagta ggcctgaatg
     3781 ctctagaaga atggtcaatt tatattgaca gacatgtaat gcagccttat tacaaagaca
     3841 ttctcccctg cctggatgga tacctgaaga cttcagcctt gtcagatgag accaagaata
     3901 actgggaagt gtcagctctt tctcgggctg cccagaaagg atttaataaa gtggtgttaa
     3961 agcatctgaa gaagacaaag aacctttcat caaacgaagc aatatcctta gaagaaataa
     4021 gaattagagt agtacaaatg cttggatctc taggaggaca aataaacaaa aatcttctga
     4081 cagtcacgtc ctcagatgag atgatgaaga gctatgtggc ctgggacaga gagaagcggc
     4141 tgagctttgc agtgcccttt agagagatga aacctgtcat tttcctggat gtgttcctgc
     4201 ctcgagtcac agaattagcg ctcacagcca gtgacagaca aactaaggtt gcagcctgtg
     4261 aacttttaca tagcatggtt atgtttatgt tgggcaaagc cacgcagatg ccagaagggg
     4321 gacagggagc cccacccatg taccagctct ataagcggac gtttcctgtg ctgcttcgac
     4381 ttgcgtgtga tgttgatcag gtgacaaggc aactgtatga gccactagtt atgcagctga
     4441 ttcactggtt cactaacaac aagaaatttg aaagtcagga tactgttgcc ttactagaag
     4501 ctatattgga tggaattgtg gaccctgttg acagtacttt aagagatttt tgtggtcggt
     4561 gtattcgaga attccttaaa tggtccatta agcaaataac accacagcag caggagaaga
     4621 gtccagtaaa caccaaatcg cttttcaagc gactttatag ccttgcgctt caccccaatg
     4681 ctttcaagag gctgggagca tcacttgcct ttaataatat ctacagggaa ttcagggaag
     4741 aagagtctct ggtggaacag tttgtgtttg aagccttggt gatatacatg gagagtctgg
     4801 ccttagcaca tgcagatgag aagtccttag gtacaattca acagtgttgt gatgccattg
     4861 atcacctatg ccgcatcatt gaaaagaagc atgtttcttt aaataaagca aagaaacgac
     4921 gtttgccgcg aggatttcca ccttccgcat cattgtgttt attggatctg gtcaagtggc
     4981 ttttagctca ttgtgggagg ccccagacag aatgtcgaca caaatccatt gaactctttt
     5041 ataaattcgt tcctttattg ccaggcaaca gatcccctaa tttgtggctg aaagatgttc
     5101 tcaaggaaga aggtgtctct tttctcatca acacctttga ggggggtggc tgtggccagc
     5161 cctcgggcat cctggcccag cccaccctct tgtaccttcg ggggccattc agcctgcagg
     5221 ccacgctatg ctggctggac ctgctcctgg ccgcgttgga gtgctacaac acgttcattg
     5281 gcgagagaac tgtaggagcg ctccaggtcc taggtactga agcccagtct tcacttttga
     5341 aagcagtggc tttcttctta gaaagcattg ccatgcatga cattatagca gcagaaaagt
     5401 gctttggcac tggggcagca ggtaacagaa caagcccaca agagggagaa aggtacaact
     5461 acagcaaatg caccgttgtg gtccggatta tggagtttac cacgactctg ctaaacacct
     5521 ccccggaagg atggaagctc ctgaagaagg acttgtgtaa tacacacctg atgagagtcc
     5581 tggtgcagac gctgtgtgag cccgcaagca taggtttcaa catcggagac gtccaggtta
     5641 tggctcatct tcctgatgtt tgtgtgaatc tgatgaaagc tctaaagatg tccccataca
     5701 aagatatcct agagacccat ctgagagaga aaataacagc acagagcatt gaggagcttt
     5761 gtgccgtcaa cttgtatggc cctgacgcgc aagtggacag gagcaggctg gctgctgttg
     5821 tgtctgcctg taaacagctt cacagagctg ggcttctgca taatatatta ccgtctcagt
     5881 ccacagattt gcatcattct gttggcacag aacttctttc cctggtttat aaaggcattg
     5941 cccctggaga tgagagacag tgtctgcctt ctctagacct cagttgtaag cagctggcca
     6001 gcggacttct ggagttagcc tttgcttttg gaggactgtg tgagcgcctt gtgagtcttc
     6061 tcctgaaccc agcggtgctg tccacggcgt ccttgggcag ctcacagggc agcgtcatcc
     6121 acttctccca tggggagtat ttctatagct tgttctcaga aacgatcaac acggaattat
     6181 tgaaaaatct ggatcttgct gtattggagc tcatgcagtc ttcagtggat aataccaaaa
     6241 tggtgagtgc cgttttgaac ggcatgttag accagagctt cagggagcga gcaaaccaga
     6301 aacaccaagg actgaaactt gcgactacaa ttctgcaaca ctggaagaag tgtgattcat
     6361 ggtgggccaa agattcccct ctcgaaacta aaatggcagt gctggcctta ctggcaaaaa
     6421 ttttacagat tgattcatct gtatctttta atacaagtca tggttcattc cctgaagtct
     6481 ttacaacata tattagtcta cttgctgaca caaagctgga tctacattta aagggccaag
     6541 ctgtcactct tcttccattc ttcaccagcc tcactggagg cagtctggag gaacttagac
     6601 gtgttctgga gcagctcatc gttgctcact tccccatgca gtccagggaa tttcctccag
     6661 gaactccgcg gttcaataat tatgtggact gcatgaaaaa gtttctagat gcattggaat
     6721 tatctcaaag ccctatgttg ttggaattga tgacagaagt tctttgtcgg gaacagcagc
     6781 atgtcatgga agaattattt caatccagtt tcaggaggat tgccagaagg ggttcatgtg
     6841 tcacacaagt aggccttctg gaaagcgtgt atgaaatgtt caggaaggat gacccccgcc
     6901 taagtttcac acgccagtcc tttgtggacc gctccctcct cactctgctg tggcactgta
     6961 gcctggatgc tttgagagaa ttcttcagca caattgtggt ggatgccatt gatgtgttga
     7021 agtccaggtt tacaaagcta aatgaatcta cctttgatac tcaaatcacc aagaagatgg
     7081 gctactataa gattctagac gtgatgtatt ctcgccttcc caaagatgat gttcatgcta
     7141 aggaatcaaa aattaatcaa gttttccatg gctcgtgtat tacagaagga aatgaactta
     7201 caaagacatt gattaaattg tgctacgatg catttacaga gaacatggca ggagagaatc
     7261 agctgctgga gaggagaaga ctttaccatt gtgcagcata caactgcgcc atatctgtca
     7321 tctgctgtgt cttcaatgag ttaaaatttt accaaggttt tctgtttagt gaaaaaccag
     7381 aaaagaactt gcttattttt gaaaatctga tcgacctgaa gcgccgctat aattttcctg
     7441 tagaagttga ggttcctatg gaaagaaaga aaaagtacat tgaaattagg aaagaagcca
     7501 gagaagcagc aaatggggat tcagatggtc cttcctatat gtcttccctg tcatatttgg
     7561 cagacagtac cctgagtgag gaaatgagtc aatttgattt ctcaaccgga gttcagagct
     7621 attcatacag ctcccaagac cctagacctg ccactggtcg ttttcggaga cgggagcagc
     7681 gggaccccac ggtgcatgat gatgtgctgg agctggagat ggacgagctc aatcggcatg
     7741 agtgcatggc gcccctgacg gccctggtca agcacatgca cagaagcctg ggcccgcctc
     7801 aaggagaaga ggattcagtg ccaagagatc ttccttcttg gatgaaattc ctccatggca
     7861 aactgggaaa tccaatagta ccattaaata tccgtctctt cttagccaag cttgttatta
     7921 atacagaaga ggtctttcgc ccttacgcga agcactggct tagccccttg ctgcagctgg
     7981 ctgcttctga aaacaatgga ggagaaggaa ttcactacat ggtggttgag atagtggcca
     8041 ctattctttc atggacaggc ttggccactc caacaggggt ccctaaagat gaagtgttag
     8101 caaatcgatt gcttaatttc ctaatgaaac atgtctttca tccaaaaaga gctgtgttta
     8161 gacacaacct tgaaattata aagacccttg tcgagtgctg gaaggattgt ttatccatcc
     8221 cttataggtt aatatttgaa aagttttccg gtaaagatcc taattctaaa gacaactcag
     8281 tagggattca attgctaggc atcgtgatgg ccaatgacct gcctccctat gacccacagt
     8341 gtggcatcca gagtagcgaa tacttccagg ctttggtgaa taatatgtcc tttgtaagat
     8401 ataaagaagt gtatgccgct gcagcagaag ttctaggact tatacttcga tatgttatag
     8461 agagaaaaaa catactggag gagtctctgt gtgaactggt tgcgaaacaa ttgaagcaac
     8521 atcagaatac tatggaggac aagtttattg tgtgcttgaa caaagtgacc aagagcttcc
     8581 ctcctcttgc agacaggttc atgaatgctg tgttctttct gctgccaaaa tttcatggag
     8641 tgttgaaaac actctgtctg gaggtggtac tttgtcgtgt ggagggaatg acagagctgt
     8701 acttccagtt aaagagcaag gacttcgttc aagtcatgag acatagagat gatgaaagac
     8761 aaaaagtatg tttggacata atttataaga tgatgccaaa gttaaaacca gtagaactcc
     8821 gagaacttct gaaccccgtt gtggaattcg tttcccatcc ttctacaaca tgtagggaac
     8881 aaatgtataa tattctcatg tggattcatg ataattacag agatccagaa agtgagacag
     8941 ataatgactc ccaggaaata tttaagttgg caaaagatgt gctgattcaa ggattgatcg
     9001 atgagaaccc tggacttcaa ttaattattc gaaatttctg gagccatgaa actaggttac
     9061 cttcaaatac cttggaccgg ttgctggcac taaattcctt atattctcct aagatagaag
     9121 tgcacttttt aagtttagca acaaattttc tgctcgaaat gaccagcatg agcccagatt
     9181 atccaaaccc catgttcgag catcctctgt cagaatgcga atttcaggaa tataccattg
     9241 attctgattg gcgtttccga agtactgttc tcactccgat gtttgtggag acccaggcct
     9301 cccagggcac tctccaggcc cgtacccagg aagggtccct ctcagctcgc tggccagtgg
     9361 cagggcagat aagggccacc cagcagcagc atgacttcac actgacacag actgcagatg
     9421 gaagaagctc atttgattgg ctgaccggga gcagcactga cccgctggtc gaccacacca
     9481 gtccctcatc tgactccttg ctgtttgccc acaagaggag tgaaaggtta cagagagcac
     9541 ccttgaagtc agtggggcct gattttggga aaaaaaggct gggccttcca ggggacgagg
     9601 tggataacaa agtgaaaggt gcggccggcc ggacggacct actacgactg cgcagacggt
     9661 ttatgaggga ccaggagaag ctcagtttga tgtatgccag aaaaggcgtt gctgagcaaa
     9721 aacgagagaa ggaaatcaag agtgagttaa aaatgaagca ggatgcccag gtcgttctgt
     9781 acagaagcta ccggcacgga gaccttcctg acattcagat caagcacagc agcctcatca
     9841 ccccgttaca ggccgtggcc cagagggacc caataattgc aaaacagctc tttagcagct
     9901 tgttttctgg aattttgaaa gagatggata aatttaagac actgtctgaa aaaaacaaca
     9961 tcactcaaaa gttgcttcaa gacttcaatc gttttcttaa taccaccttc tctttctttc
    10021 caccctttgt ctcttgtatt caggacatta gctgtcagca cgcagccctg ctgagcctcg
    10081 acccagcggc tgttagcgct ggttgcctgg ccagcctaca gcagcccgtg ggcatccgcc
    10141 tgctagagga ggctctgctc tgcctgctgc ctgctgagct gcccgccaag cgagtccgtg
    10201 ggaaggcccg cccccctcct gatgtcctca gatgggtgga gcttgctaag ctgtatagat
    10261 caattggaga atacgacgtc ctccgtggga tttttaccag tgagatagga acaaagcaaa
    10321 tcactcagag tgcattatta gcagaagcca gaagtgatta ttctgaagct gctaagcagt
    10381 atgatgaggc tctcaataaa caagactggg tagatggtga gcccacagaa gccgagaagg
    10441 atttttggga acttgcatcc cttgactgtt acaaccacct tgctgagtgg aaatcacttg
    10501 aatactgttc tacagccagt atagacagtg agaacccccc agacctaaat aaaatctgga
    10561 gtgaaccatt ttatcaggaa acatatctac cttacatgat ccgcagcaag ctgaagctgc
    10621 tgctccaggg agaggctgac cagtccctgc tgacatttat tgacaaagct atgcacgggg
    10681 agctccagaa ggcgattcta gagcttcatt acagtcaaga gctgagtctg ctttacctcc
    10741 tgcaagatga tgttgacaga gccaaatatt acattcaaaa tggcattcag agttttatgc
    10801 agaattattc tagtattgat gtcctcttac accaaagtag actcaccaaa ttgcagtctg
    10861 tacaggcttt aacagaaatt caggagttca tcagctttat aagcaaacaa ggcaatttat
    10921 catctcaagt tccccttaag agacttctga acacctgggc aaacagatat ccagatgcta
    10981 aaatggaccc aatgaacatc tgggatgaca tcatcacaaa tcgatgtttc tttctcagca
    11041 aaatagagga gaagcttacc cctcttccag aagataatag tatgaatgtg gatcaagatg
    11101 gagaccccag tgacaggatg gaagtgcaag agcaggaaga agatatcagc tccctgatca
    11161 ggagttgcaa gttttccatg aaaatgaaga tgatagacag tgcccggaag cagaacaatt
    11221 tctcacttgc tatgaaacta ctgaaggagc tgcataaaga gtcaaaaacc agagacgatt
    11281 ggctggtgag ctgggtgcag agctactgcc gcctgagcca ctgccggagc cggtcccagg
    11341 gctgctctga gcaggtgctc actgtgctga aaacagtctc tttgttggat gagaacaacg
    11401 tgtcaagcta cttaagcaaa aatattctgg ctttccgtga ccagaacatt ctcttgggta
    11461 caacttacag gatcatagcg aatgctctca gcagtgagcc agcctgcctt gctgaaatcg
    11521 aggaggacaa ggctagaaga atcttagagc tttctggatc cagttcagag gattcagaga
    11581 aggtgatcgc gggtctgtac cagagagcat tccagcacct ctctgaggct gtgcaggcgg
    11641 ctgaggagga ggcccagcct ccctcctgga gctgtgggcc tgcagctggg gtgattgatg
    11701 cttacatgac gctggcagat ttctgtgacc aacagctgcg caaggaggaa gagaatgcat
    11761 cagttactga ttctgcagaa ctgcaggcgt atccagcact tgtggtggag aaaatgttga
    11821 aagctttaaa attaaattcc aatgaagcca gattgaagtt tcctagatta cttcagatta
    11881 tagaacggta tccagaggag actttgagcc tcatgacaaa agagatctct tccgttccct
    11941 gctggcagtt catcagctgg atcagccaca tggtggcctt actggacaaa gaccaagccg
    12001 ttgctgttca gcactctgtg gaagaaatca ctgataacta cccgcaggct attgtttatc
    12061 ccttcatcat aagcagcgaa agctattcct tcaaggatac ttctactggt cataagaata
    12121 aggagtttgt ggcaaggatt aaaagtaagt tggatcaagg aggagtgatt caagatttta
    12181 ttaatgcctt agatcagctc tctaatcctg aactgctctt taaggattgg agcaatgatg
    12241 taagagctga actagcaaaa acccctgtaa ataaaaaaaa cattgaaaaa atgtatgaaa
    12301 gaatgtatgc agccttgggt gacccaaagg ctccaggcct gggggccttt agaaggaagt
    12361 ttattcagac ttttggaaaa gaatttgata aacattttgg gaaaggaggt tctaaactac
    12421 tgagaatgaa gctcagtgac ttcaacgaca ttaccaacat gctactttta aaaatgaaca
    12481 aagactcaaa gccccctggg aatctgaaag aatgttcacc ctggatgagc gacttcaaag
    12541 tggagttcct gagaaatgag ctggagattc ccggtcagta tgacggtagg ggaaagccat
    12601 tgccagagta ccacgtgcga atcgccgggt ttgatgagcg ggtgacagtc atggcgtctc
    12661 tgcgaaggcc caagcgcatc atcatccgtg gccatgacga gagggaacac cctttcctgg
    12721 tgaagggtgg cgaggacctg cggcaggacc agcgcgtgga gcagctcttc caggtcatga
    12781 atgggatcct ggcccaagac tccgcctgca gccagagggc cctgcagctg aggacctata
    12841 gcgttgtgcc catgacctcc aggttaggat taattgagtg gcttgaaaat actgttacct
    12901 tgaaggacct tcttttgaac accatgtccc aagaggagaa ggcggcttac ctgagtgatc
    12961 ccagggcacc gccgtgtgaa tataaagatt ggctgacaaa aatgtcagga aaacatgatg
    13021 ttggagctta catgctaatg tataagggcg ctaatcgtac tgaaacagtc acgtctttta
    13081 gaaaacgaga aagtaaagtg cctgctgatc tcttaaagcg ggccttcgtg aggatgagta
    13141 caagccctga ggctttcctg gcgctccgct cccacttcgc cagctctcac gctctgatat
    13201 gcatcagcca ctggatcctc gggattggag acagacatct gaacaacttt atggtggcca
    13261 tggagactgg tggcgtgatc gggatcgact ttgggcacgc gtttggatcc gctgatcagt
    13321 ttctgccagt ccctgagttg atgccttttc ggctaactcg ccagtttatc aatctgatgt
    13381 taccaatgaa agaaacgggc cttatgtaca gcatcatggt acacgcactc cgggccttcc
    13441 gctcagaccc tggcctgctc accaacacca tggatgtgtt tgtcaaggag ccctcctttg
    13501 attggaaaaa ttttgaacag aaaatgctga aaaaaggagg gtcatggatt caagaaataa
    13561 atgttgctga aaaaaattgg tacccccgac agaaaatatg ttacgctaag agaaagttag
    13621 caggtgccaa tccagcagtc attacttgtg atgagctact cctgggtcat gagaaggccc
    13681 ctgccttcag agactatgtg gctgtggcac gaggaagcaa agatcacaac attcgtgccc
    13741 aagaaccaga gagtgggctt tcagaagaga ctcaagtgaa gtgcctgatg gaccaggcaa
    13801 cagaccccaa catccttggc agaacctggg aaggatggga gccctggatg tgaggtctgc
    13861 ggccgcggat cccgggtggc atccctgtga cccctcccca gtgcctctcc tggccctgga
    13921 agttgccact ccagtgccca ccagccttgt cctaataaaa ttaagttgca tcattttgtc
    13981 tgactaggtg tccttctata atattatggg gtggaggggg gtggtatgga gcaaggggca
    14041 agttgggaag acaacctgta gggcctgcgg ggtctattgg gaaccaagct ggagtgcagt
    14101 ggcacaatct tggctcactg caatctccgc ctcctgggtt caagcgattc tcctgcctca
    14161 gcctcccgag ttgttgggat tccaggcatg catgaccagg ctcagctaat ttttgttttt
    14221 ttggtagaga cggggtttca ccatattggc caggctggtc tccaactcct aatctcaggt
    14281 gatctaccca ccttggcctc ccaaattgct gggattacag gcgtgaacca ctgctccctt
    14341 ccctgtcctt ctgattttaa aataactata ccagcaggag gacgtccaga cacagcatag
    14401 gctacctggc catgcccaac cggtgggaca tttgagttgc ttgcttggca ctgtcctctc
    14461 atgcgttggg tccactcagt agatgcctgt tgaattgggt acgcggccag cttggctgtg
    14521 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
    14581 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
    14641 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
    14701 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
    14761 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
    14821 aggaggcttt tttggaggcc taggcttttg caaaaagctc ctcgaggaac tgaaaaacca
    14881 gaaagttaat tccctatagt gagtcgtatt aaattcgtaa tcatgtcata gctgtttcct
    14941 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
    15001 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
    15061 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
    15121 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
    15181 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
    15241 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
    15301 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
    15361 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
    15421 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
    15481 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
    15541 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
    15601 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
    15661 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
    15721 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
    15781 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
    15841 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
    15901 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
    15961 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
    16021 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
    16081 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
    16141 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
    16201 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
    16261 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
    16321 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
    16381 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
    16441 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
    16501 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
    16561 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
    16621 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
    16681 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
    16741 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
    16801 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    16861 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    16921 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    16981 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    17041 ggggttccgc gca
//
