LOCUS       sequence-190317-        9402 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express GFP-tagged Arabidopsis SEIPIN2 gene in yeast cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9402)
  AUTHORS   Cai Y, Goodman JM, Pyc M, Mullen RT, Dyer JM, Chapman KD
  TITLE     Arabidopsis SEIPIN Proteins Modulate Triacylglycerol Accumulation 
            and Influence Lipid Droplet Proliferation.
  JOURNAL   Plant Cell. 2015 Sep;27(9):2616-36. doi: 10.1105/tpc.15.00588. Epub 
            2015 Sep 11.
  PUBMED    26362606
REFERENCE   2  (bases 1 to 9402)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1105/tpc.15.00588"; journalName: "Plant Cell"; date: "2015-09"; 
            volume: "27"; issue: "9"; pages: "2616-36"
COMMENT     Sequence Label: pRS315-PGK-SEIPIN2
FEATURES             Location/Qualifiers
     source          1..9402
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     33..49
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     33..49
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      1745..2015
                     /gene="S. cerevisae PGK1"
                     /label=PGK1 terminator
                     /note="transcription terminator for 3-phosphoglycerate 
                     kinase gene"
     promoter        complement(2053..2071)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2092..2108)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2092..2108)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2105..2127)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2116..2132
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2140..2146,2147..2164,2165..2170))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2185..2206
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2323..2340)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2494..3082)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2574..2593)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3253..4044,4045..4113))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3876..3895
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4114..4218)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    4255..4758
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     4800..4818
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4856..4878)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4978..4997
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(5491..6585)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(6586..6993)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     rep_origin      complement(7294..7749)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7431..7452)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7643..7662
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7875..7897
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7889..7906
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7890..7906
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7913..7932
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7913..7931
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 attatctact ttttacaaca aatataaaac aatctagaac tagtggatcc atggactccg
       61 agtccgagtc cgagtcaaac cctagcacca cggacgaatt cgatcgattc ctcgacgcac
      121 ctgacgaatt ttactacgat tgcttaccga ttcggtccaa ttcccatcaa ccttcgtctc
      181 tcttacggcg gcgaaagtcg gcacatcgcc gggatttaat ttcgtccgat attgaaacgg
      241 agccatcttc ttcttccgat ggatttgatg tcggtgagaa atccagctat gtggaaaaga
      301 acgctgagct tagaggggat attgatacaa gcgacgtaat tgaatcaact aaggactcaa
      361 ttgatctatc ttcagaaaag gagaacgatt tggatgtaat tagttcgtct gggaatgata
      421 tggatgtaat tgattcgggt cggaaccggg ttgacccgtt tcaggaagaa tccacggtga
      481 ctaccgtgag cagcgatgat caaggagacg acgactacgc cggatcggta ccacagtttc
      541 gggagccacc taattccact gagtggtctc tccttgggtt tttggttggg ctagtgatca
      601 aagcaatcga atttcaagtc agtttcatga caagcttact cacatttcct ccatggcttc
      661 tccgtaactg ttttctcttc tttttcgatc cctttagcac gattaggttt ggcagacgct
      721 ttttaatggc aagagtagca ggaataagtg atatgatctt tggttatatg aatcctttta
      781 ggttaaaaga tacaaaacaa atgttgagta ttgtgtgcaa gtttggttgg ggtatgtttt
      841 gggctgttta tgtaggcatt gtgttgtttg gcttgttggt ttcgtcgctt atgattggtg
      901 gttatgtgat taaccgtata gcggataaac catttgaggt caaggagacc ttgaattttg
      961 attacaccaa gaatagtcca gaagcatatg tgcccatatc ctcgtgtgct ggtgttgaat
     1021 gtgagggaag ttgcaaggaa agtaatgaaa tgtcgaagat caggggtttg cgagttatcc
     1081 ctcgagacca gaagttagat atcattcttt caatgacatt acctgagtct gcgtacaata
     1141 aaaatctcgg catgtttcag gttcgggtgg acttcttatc tgtggatggt caaacgattg
     1201 ctagtataag gcgtccgtgc atgttaagat tcagaagtga gcctattcgt cttgttcaga
     1261 cattcttcaa agtggttccg ttagtgacag gatatgtctc tgagatccaa accttgagct
     1321 tgaagttgaa aggctttgta gaaaaggaca ttcccactgc ttgcttaaag ataataatcg
     1381 aacaacgggc agaatttcga cccggagcag gtattccaga gctgtacgat gcatccctat
     1441 cggttgaatc cggtcttccc ttcttcagaa aaattatatg gaaatggcgg aagactttgt
     1501 tcgtctggat cagcatgagt ttgttcatta cggaattgct tttcacgttg gtttgttgta
     1561 gacctcttat catcccaaga acacaaccta gagacagatc gccttcaaac ccaactggag
     1621 tctggaggta gctgcaggaa ttcgatatca agcttcccat gtctctactg gtggtggtgc
     1681 ttctttggaa ttattggaag gtaaggaatt gccaggtgtt gctttcttat ccgaaaagaa
     1741 ataaattgaa ttgaattgaa atcgatagat caattttttt cttttctctt tccccatcct
     1801 ttacgctaaa ataatagttt attttatttt ttgaatattt tttatttata tacgtatata
     1861 tagactatta tttatctttt aatgattatt aagattttta ttaaaaaaaa attcgctcct
     1921 cttttaatgc ctttatgcag tttttttttc ccattcgata tttctatgta cgggttcagc
     1981 gtattttaag tttaataact cgaaaattct gcgttcgtta ctcgaggggg ggcccggtac
     2041 cagcttttgt tccctttagt gagggttaat ttcgagcttg gcgtaatcat ggtcatagct
     2101 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     2161 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     2221 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     2281 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     2341 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     2401 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     2461 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     2521 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     2581 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     2641 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     2701 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     2761 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     2821 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     2881 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     2941 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     3001 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     3061 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     3121 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     3181 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     3241 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     3301 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     3361 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     3421 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     3481 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     3541 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     3601 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     3661 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     3721 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     3781 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     3841 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     3901 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     3961 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     4021 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     4081 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     4141 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     4201 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgggtcct tttcatcacg
     4261 tgctataaaa ataattataa tttaaatttt ttaatataaa tatataaatt aaaaatagaa
     4321 agtaaaaaaa gaaattaaag aaaaaatagt ttttgttttc cgaagatgta aaagactcta
     4381 gggggatcgc caacaaatac taccttttat cttgctcttc ctgctctcag gtattaatgc
     4441 cgaattgttt catcttgtct gtgtagaaga ccacacacga aaatcctgtg attttacatt
     4501 ttacttatcg ttaatcgaat gtatatctat ttaatctgct tttcttgtct aataaatata
     4561 tatgtaaagt acgctttttg ttgaaatttt ttaaaccttt gtttattttt ttttcttcat
     4621 tccgtaactc ttctaccttc tttatttact ttctaaaatc caaatacaaa acataaaaat
     4681 aaataaacac agagtaaatt cccaaattat tccatcatta aaagatacga ggcgcgtgta
     4741 agttacaggc aagcgatccg tcctaagaaa ccattattat catgacatta acctataaaa
     4801 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
     4861 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     4921 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
     4981 catcagagca gattgtactg agagtgcacc atatcgacta cgtcgttaag gccgtttctg
     5041 acagagtaaa attcttgagg gaactttcac cattatggga aatggttcaa gaaggtattg
     5101 acttaaactc catcaaatgg tcaggtcatt gagtgttttt tatttgttgt attttttttt
     5161 ttttagagaa aatcctccaa tatataaatt aggaatcata gtttcatgat tttctgttac
     5221 acctaacttt ttgtgtggtg ccctcctcct tgtcaatatt aatgttaaag tgcaattctt
     5281 tttccttatc acgttgagcc attagtatca atttgcttac ctgtattcct ttacatcctc
     5341 ctttttctcc ttcttgataa atgtatgtag attgcgtata tagtttcgtc taccctatga
     5401 acatattcca ttttgtaatt tcgtgtcgtt tctattatga atttcattta taaagtttat
     5461 gtacaaatat cataaaaaaa gagaatcttt ttaagcaagg attttcttaa cttcttcggc
     5521 gacagcatca ccgacttcgg tggtactgtt ggaaccacct aaatcaccag ttctgatacc
     5581 tgcatccaaa acctttttaa ctgcatcttc aatggcctta ccttcttcag gcaagttcaa
     5641 tgacaatttc aacatcattg cagcagacaa gatagtggcg atagggttga ccttattctt
     5701 tggcaaatct ggagcagaac cgtggcatgg ttcgtacaaa ccaaatgcgg tgttcttgtc
     5761 tggcaaagag gccaaggacg cagatggcaa caaacccaag gaacctggga taacggaggc
     5821 ttcatcggag atgatatcac caaacatgtt gctggtgatt ataataccat ttaggtgggt
     5881 tgggttctta actaggatca tggcggcaga atcaatcaat tgatgttgaa ccttcaatgt
     5941 agggaattcg ttcttgatgg tttcctccac agtttttctc cataatcttg aagaggccaa
     6001 aacattagct ttatccaagg accaaatagg caatggtggc tcatgttgta gggccatgaa
     6061 agcggccatt cttgtgattc tttgcacttc tggaacggtg tattgttcac tatcccaagc
     6121 gacaccatca ccatcgtctt cctttctctt accaaagtaa atacctccca ctaattctct
     6181 gacaacaacg aagtcagtac ctttagcaaa ttgtggcttg attggagata agtctaaaag
     6241 agagtcggat gcaaagttac atggtcttaa gttggcgtac aattgaagtt ctttacggat
     6301 ttttagtaaa ccttgttcag gtctaacact accggtaccc catttaggac cacccacagc
     6361 acctaacaaa acggcatcag ccttcttgga ggcttccagc gcctcatctg gaagtggaac
     6421 acctgtagca tcgatagcag caccaccaat taaatgattt tcgaaatcga acttgacatt
     6481 ggaacgaaca tcagaaatag ctttaagaac cttaatggct tcggctgtga tttcttgacc
     6541 aacgtggtca cctggcaaaa cgacgatctt cttaggggca gacattagaa tggtatatcc
     6601 ttgaaatata tatatatata ttgctgaaat gtaaaaggta agaaaagtta gaaagtaaga
     6661 cgattgctaa ccacctattg gaaaaaacaa taggtcctta aataatattg tcaacttcaa
     6721 gtattgtgat gcaagcattt agtcatgaac gcttctctat tctatatgaa aagccggttc
     6781 cggcgctctc acctttcctt tttctcccaa tttttcagtt gaaaaaggta tatgcgtcag
     6841 gcgacctctg aaattaacaa aaaatttcca gtcatcgaat ttgattctgt gcgatagcgc
     6901 ccctgtgtgt tctcgttatg ttgaggaaaa aaataatggt tgctaagaga ttcgaactct
     6961 tgcatcttac gatacctgag tattcccaca gttaactgcg gtcaagatat ttcttgaatc
     7021 aggcgcctta gaccgctcgg ccaaacaacc aattacttgt tgagaaatag agtataatta
     7081 tcctataaat ataacgtttt tgaacacaca tgaacaagga agtacaggac aattgatttt
     7141 gaagagaatg tggattttga tgtaattgtt gggattccat ttttaataag gcaataatat
     7201 taggtatgta gatatactag aagttctcct cgaccggtcg atatgcggtg tgaaataccg
     7261 cacagatgcg taaggagaaa ataccgcatc aggaaattgt aagcgttaat attttgttaa
     7321 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     7381 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
     7441 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     7501 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     7561 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     7621 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     7681 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     7741 agggcgcgtc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
     7801 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
     7861 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat tgtaatacga
     7921 ctcactatag ggcgaattgg agctccaccg cggtctaact gatctatcca aaactgaaaa
     7981 ttacattctt gattaggttt atcacaggca aatgtaattt gtggtatttt gccgttcaaa
     8041 atctgtagaa ttttctcatt ggtcacatta caacctgaaa atactttatc tacaatcata
     8101 ccattcttat aacatgtccc cttaatacta ggatcaggca tgaacgcatc acagacaaaa
     8161 tcttcttgac aaacgtcaca attgatccct ccccatccgt tatcacaatg acaggtgtca
     8221 ttttgtgctc ttatgggacg atccttatta ccgctttcat ccggtgatag accgccacag
     8281 aggggcagag agcaatcatc acctgcaaac ccttctatac actcacatct accagtgtac
     8341 gaattgcatt cagaaaactg tttgcattca aaaataggta gcatacaatt aaaacatggc
     8401 gggcatgtat cattgccctt atcttgtgca gttagacgcg aatttttcga agaagtacct
     8461 tcaaagaatg gggtcttatc ttgttttgca agtaccactg agcaggataa taatagaaat
     8521 gataatatac tatagtagag ataacgtcga tgacttccca tactgtaatt gcttttagtt
     8581 gtgtattttt agtgtgcaag tttctgtaaa tcgattaatt tttttttctt tcctcttttt
     8641 attaacctta atttttattt tagattcctg acttcaactc aagacgcaca gatattataa
     8701 catctgcata ataggcattt gcaagaatta ctcgtgagta aggaaagagt gaggaactat
     8761 cgcatacctg catttaaaga tgccgatttg ggcgcgaatc ctttattttg gcttcaccct
     8821 catactatta tcagggccag aaaaaggaag tgtttccctc cttcttgaat tgatgttacc
     8881 ctcataaagc acgtggcctc ttatcgagaa agaaattacc gtcgctcgtg atttgtttgc
     8941 aaaaagaaca aaactgaaaa aacccagaca cgctcgactt cctgtcttcc tattgattgc
     9001 agcttccaat ttcgtcacac aacaaggtcc tagcgacggc tcacaggttt tgtaacaagc
     9061 aatcgaaggt tctggaatgg cgggaaaggg tttagtacca catgctatga tgcccactgt
     9121 gatctccaga gcaaagttcg ttcgatcgta ctgttactct ctctctttca aacagaattg
     9181 tccgaatcgt gtgacaacaa cagcctgttc tcacacactc ttttcttcta accaaggggg
     9241 tggtttagtt tagtagaacc tcgtgaaact tacatttaca tatatataaa cttgcataaa
     9301 ttggtcaatg caagaaatac atatttggtc ttttctaatt cgtagttttt caagttctta
     9361 gatgctttct ttttctcttt tttacagatc atcaaggaag ta
//
