LOCUS       sequence-19045-e         759 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Jiang Z, Georgel P, Li C, Choe J, Crozat K, Rutschmann S, Du X, 
            Bigby T, Mudd S, Sovath S, Wilson IA, Olson A, Beutler B
  TITLE     Details of Toll-like receptor:adapter interaction revealed by 
            germ-line mutagenesis.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Jul 18. 103(29):10961-6.
  PUBMED    16832055
REFERENCE   2  (bases 1 to 759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2006 Jul 18. 103(29):10961-6."
COMMENT     Sequence Label: pCMV-HA-MyD88-I179N
FEATURES             Location/Qualifiers
     source          1..759
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        225..243
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     225..242
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     256..275
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             330..419
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
ORIGIN
        1 gtgaaccgtc agaacgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
       61 cgggaccgat ccagcctccg cggccgggaa cggtgcattg gaacgcggat tccccgtgcc
      121 aagagtgacg taagtaccgc ctatagagtc tataggccca cccccttggc ttcgttagaa
      181 cgcggctaca attaatacat aaccttatgt atcatacaca tacgatttag gtgacactat
      241 agaataacat ccactttgcc tttctctcca caggtgtcca ctcccaggtc caactgcacc
      301 tcggttctat cgatatgggc cgcatctttt acccatacga tgttcctgac tatgcgggct
      361 atccctatga cgtcccggac tatgcaggat cctatccata tgacgttcca gattacgctg
      421 ctcagcgcga attcgcggcc gcagctgcag gaggtcccgg cgcggggtct gcggccccgg
      481 tctcctccac atcctccctt cccctggctg ctctcaacat gcgagtgcgg cgccgcctgt
      541 ctctgttctt gaacgtgcgg acacaggtgg cggccgactg gaccgcgctg gcggaggaga
      601 tggactttga gtacttggag atccggcaac tggagacaca agcggacccc actggcaggc
      661 tgctggacgc ctggcaggga cgccctggcg cctctgtagg ccgactgctc gagctgctta
      721 ccaagctggg ccgcgacgac gtgctgctgg agctgggac
//
