LOCUS       sequence-190476-        9237 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Shuttle vector for us in AdEasy System. For expression of transgenes
            under a CMV promoter when a GFP tracer is desired..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9237)
  AUTHORS   He TC, Zhou S, da Costa LT, Yu J, Kinzler KW, Vogelstein B
  TITLE     A simplified system for generating recombinant adenoviruses.
  JOURNAL   Proc Natl Acad Sci U S A. 1998 Mar 3. 95(5):2509-14.
  PUBMED    9482916
REFERENCE   2  (bases 1 to 9237)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 1998 Mar 3. 95(5):2509-14."
COMMENT     Sequence Label: pAdTrack-CMV
FEATURES             Location/Qualifiers
     source          1..9237
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    5..126
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        212..515
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        516..719
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      758..767
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(764..766,767..769,770..1480)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(764..766,767..769,770..1480)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(809..830)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1070..1089)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1417..1438
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    1619..1740
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_signal     1883..2033
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     repeat_region   2121..2223
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     rep_origin      2261..2641
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2348..2367)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2558..2579
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(2689..3483)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(2689..3483)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2800..2819)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3410..3429
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     rep_origin      4315..4903
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4804..4823
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5057..5074
                     /label=L4440
                     /note="L4440 vector, forward primer"
     repeat_region   5163..5265
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     enhancer        8524..8827
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8828..9031
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
       61 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
      121 atcttaacgc ggaggtttat cgacgatctg ctagtgatta atagtaatca attacggggt
      181 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
      241 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
      301 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
      361 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
      421 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
      481 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
      541 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
      601 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
      661 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg
      721 gtttagtgaa ccgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga
      781 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
      841 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
      901 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
      961 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     1021 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     1081 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     1141 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     1201 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     1261 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     1321 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     1381 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     1441 cgccgccggg atcactctcg gcatggacga gctgtacaag tccggactca gatccaccgg
     1501 atctagataa ctgatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     1561 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     1621 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     1681 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     1741 acgcgatcaa gctagcttgc tagactcgac tgactataat aataaaacgc caactttgac
     1801 ccggaacgcg gaaaacacct gagaaaaaca cctgggcgag tctccacgta aacggtcaaa
     1861 gtccccgcgg ccctagacaa atattacgcg ctatgagtaa cacaaaatta ttcagatttc
     1921 acttcctctt attcagtttt cccgcgaaaa tggccaaatc ttactcggtt acgcccaaat
     1981 ttactacaac atccgcctaa aaccgcgcga aaattgtcac ttcctgtgta caccggcgca
     2041 caccaaaaac gtcacttttg ccacatccgt cgcttacatg tgttccgcca cacttgcaac
     2101 atcacacttc cgccacacta ctacgtcacc cgccccgttc ccacgccccg cgccacgtca
     2161 caaactccac cccctcatta tcatattggc ttcaatccaa aataaggtat attattgatg
     2221 atgttaatta agaattaatt cgatcctgaa tggcgaatgg acgcgccctg tagcggcgca
     2281 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     2341 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     2401 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     2461 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     2521 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     2581 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     2641 gcctattggt taaaaaatga gctgatttaa caaaaatttt aacaaaattc agaagaactc
     2701 gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac
     2761 gaggaagcgg tcagcccatt cgccgccaag ctcttcagca atatcacggg tagccaacgc
     2821 tatgtcctga tagcggtccg ccacacccag ccggccacag tcgatgaatc cagaaaagcg
     2881 gccattttcc accatgatat tcggcaagca ggcatcgcca tgggtcacga cgagatcctc
     2941 gccgtcgggc atgctcgcct tgagcctggc gaacagttcg gctggcgcga gcccctgatg
     3001 ctcttcgtcc agatcatcct gatcgacaag accggcttcc atccgagtac gtgctcgctc
     3061 gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg
     3121 ccgcattgca tcagccatga tggatacttt ctcggcagga gcaaggtgag atgacaggag
     3181 atcctgcccc ggcacttcgc ccaatagcag ccagtccctt cccgcttcag tgacaacgtc
     3241 gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc
     3301 ttgcagttca ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg
     3361 cgctgacagc cggaacacgg cggcatcaga gcagccgatt gtctgttgtg cccagtcata
     3421 gccgaatagc ctctccaccc aagcggccgg agaacctgcg tgcaatccat cttgttcaat
     3481 catgcgaaac gatcctcatc ctgtctcttg atcagagctt gatcccctgc gccatcagat
     3541 ccttggcggc aagaaagcca tccagtttac tttgcagggc ttcccaacct taccagaggg
     3601 cgccccagct ggcaattccg gttcgcttgc tgtccataaa accgcccagt ctagctatcg
     3661 ccatgtaagc ccactgcaag ctacctgctt tctctttgcg cttgcgtttt cccttgtcca
     3721 gatagcccag tagctgacat tcatccgggg tcagcaccgt ttctgcggac tggctttcta
     3781 cgtgaaaagg atctaggtga agatcctttt tgataatctc atggctgcag caatggcaac
     3841 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     3901 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     3961 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     4021 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     4081 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     4141 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     4201 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     4261 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     4321 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     4381 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     4441 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     4501 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     4561 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     4621 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     4681 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     4741 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     4801 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     4861 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     4921 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     4981 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     5041 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta
     5101 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggatccatg catgttaatt
     5161 aacatcatca ataatatacc ttattttgga ttgaagccaa tatgataatg agggggtgga
     5221 gtttgtgacg tggcgcgggg cgtgggaacg gggcgggtga cgtaggtttt agggcggagt
     5281 aacttgtatg tgttgggaat tgtagttttc ttaaaatggg aagtgacgta acgtgggaaa
     5341 acggaagtga cgatttgagg aagttgtggg ttttttggct ttcgtttctg ggcgtaggtt
     5401 cgcgtgcggt tttctgggtg ttttttgtgg actttaaccg ttacgtcatt ttttagtcct
     5461 atatatactc gctctgcact tggccctttt ttacactgtg actgattgag ctggtgccgt
     5521 gtcgagtggt gtttttttaa taggttttct tttttactgg taaggctgac tgttatggct
     5581 gccgctgtgg aagcgctgta tgttgttctg gagcgggagg gtgctatttt gcctaggcag
     5641 gagggttttt caggtgttta tgtgtttttc tctcctatta attttgttat acctcctatg
     5701 ggggctgtaa tgttgtctct acgcctgcgg gtatgtattc ccccgggcta tttcggtcgc
     5761 tttttagcac tgaccgatgt gaatcaacct gatgtgttta ccgagtctta cattatgact
     5821 ccggacatga ccgaggagct gtcggtggtg ctttttaatc acggtgacca gtttttttac
     5881 ggtcacgccg gcatggccgt agtccgtctt atgcttataa gggttgtttt tcctgttgta
     5941 agacaggctt ctaatgttta aatgtttttt tgttatttta ttttgtgttt atgcagaaac
     6001 ccgcagacat gtttgagaga aaaatggtgt ctttttctgt ggtggttccg gagcttacct
     6061 gcctttatct gcatgagcat gactacgatg tgctttcttt tttgcgcgag gctttgcctg
     6121 attttttgag cagcaccttg cattttatat cgccgcccat gcaacaagct attgaattcg
     6181 tttaaactcc ctctcaagtc tgtatacggg gacacggaca gccttttcgt caccgagcgt
     6241 ggacaccggc tcatggaaac cagaggtaag aaacgcatca aaaagcatgg gggaaacctg
     6301 gtttttgacc ccgaacggcc agagctcacc tggctcgtgg aatgcgagac cgtctgcggg
     6361 gcctgcggcg cggatgccta ctccccggaa tcggtatttc tcgcgcccaa gctctacgcc
     6421 ctcaaaagtc tgcactgccc ctcgtgcggc gcctcctcca agggcaagct gcgcgccaag
     6481 ggccacgccg cggaggggct ggactatgac accatggtca aatgctacct ggccgacgcg
     6541 cagggcgaag accggcagcg cttcagcacc agcaggacca gcctcaagcg caccctggcc
     6601 agcgcgcagc ccggagcgca ccccttcacc gtgacccaga ctacgctgac gaggaccctg
     6661 cgcccgtgga aagacatgac cctggcccgt ctggacgagc accgactact gccgtacagc
     6721 gaaagccgcc ccaacccgcg aaacgaggag atatgctgga tcgagatgcc gtagagcagg
     6781 tgaccgagct gtgggaccgc ctggaactgc ttggtcaaac gctcaaaagc atgcctacgg
     6841 cggacggtct caaaccgttg aaaaactttg cttccttgca agaactgcta tcgctgggcg
     6901 gcgagcgcct tctggcggat ttggtcaggg aaaacatgcg agtcagggac atgcttaacg
     6961 aagtggcccc cctgctcagg gatgacggca gctgcagctc tcttaactac cagttgcagc
     7021 cggtaatagg tgtgatttac gggcccaccg gctgcggtaa gtcgcagctg ctcaggaacc
     7081 tgctttcttc ccagctgatc tcccctaccc cggaaaccgt tttcttcatc gccccgcagg
     7141 tagacatgat ccccccatct gaactcaaag cgtgggaaat gcaaatctgt gagggtaact
     7201 acgcccctgg gccggatgga accattatac cgcagtctgg caccctccgc ccgcgctttg
     7261 taaaaatggc ctatgacgat ctcatcctgg aacacaacta tgacgttagt gatcccagaa
     7321 atatcttcgc ccaggccgcc gcccgtgggc ccattgccat cattatggac gaatgcatgg
     7381 aaaatcttgg aggtcacaag ggcgtctcca agttcttcca cgcatttcct tctaagctac
     7441 atgacaaatt tcccaagtgc accggataca ctgtgctggt ggttctgcac aacatgaatc
     7501 cccggaggga tatggctggg aacatagcca acctaaaaat acagtccaag atgcatctca
     7561 tatccccacg tatgcaccca tcccagctta accgctttgt aaacacttac accaagggcc
     7621 tgcccctggc aatcagcttg ctactgaaag acatttttag gcaccacgcc cagcgctcct
     7681 gctacgactg gatcatctac aacaccaccc cgcagcatga agctctgcag tggtgctacc
     7741 tccaccccag agacgggctt atgcccatgt atctgaacat ccagagtcac ctttaccacg
     7801 tcctggaaaa aatacacagg accctcaacg accgagaccg ctggtcccgg gcctaccgcg
     7861 cgcgcaaaac ccctaaataa agacagcaag acacttgctt gatccaaatc caaacagagt
     7921 ctggtttttt atttatgttt taaaccgcat tgggagggga ggaagccttc agggcagaaa
     7981 cctgctggcg cagatccaac agctgctgag aaacgacatt aagttcccgg gtcaaagaat
     8041 ccaattgtgc caaaagagcc gtcaacttgt catcgcgggc ggatgaacgg gaagctgcac
     8101 tgcttgcaag cgggctcagg aaagcaaagt cagtcacaat cccgcgggcg gtggctgcag
     8161 cggctgaagc ggcggcggag gctgcagtct ccaacggcgt tccagacacg gtctcgtagg
     8221 tcaaggtagt agagtttgcg ggcaggacgg ggcgaccatc aatgctggag cccatcacat
     8281 tctgacgcac cccggcccat gggggcatgc gcgttgtcaa atatgagctc acaatgcttc
     8341 catcaaacga gttggtgctc atggcggcgg cggctgctgc aaaacagata caaaactaca
     8401 taagaccccc accttatata ttctttccca cccttaacca cgcccagatc ctctagcagt
     8461 gataaacgtc taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
     8521 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     8581 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     8641 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     8701 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     8761 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     8821 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
     8881 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
     8941 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
     9001 tgtacggtgg gaggtctata taagcagagc tggtttagtg aaccgtcaga tccgctagag
     9061 atctggtacc gtcgacgcgg ccgctcgagc ctaagcttct agataagata tccgatccac
     9121 cggatctaga taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt
     9181 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgt
//
