LOCUS       sequence-190497-        6482 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6482)
  AUTHORS   Godar S, Ince TA, Bell GW, Feldser D, Donaher JL, Bergh J, Liu A, 
            Miu K, Watnick RS, Reinhardt F, McAllister SS, Jacks T, Weinberg RA
  TITLE     Growth-inhibitory and tumor- suppressive functions of p53 depend on 
            its repression of CD44 expression.
  JOURNAL   Cell. 2008 Jul 11. 134(1):62-73.
  PUBMED    18614011
REFERENCE   2  (bases 1 to 6482)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2008 
            Jul 11. 134(1):62-73."
COMMENT     Sequence Label: CD44S pBabe-puro
FEATURES             Location/Qualifiers
     source          1..6482
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(594..1385,1386..1454))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1217..1236
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1455..1559)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             1590..2059
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    2124..2323
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             2524..2940
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     2870..2892
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     2908..2924
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(4394..4414)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4399..4728
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4579..4714
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4738..5337
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4738..4757)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5234..5254
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5558..6027
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(6157..6174)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(6328..6482,1..434))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6408..6427)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
       61 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
      121 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
      181 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
      241 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
      301 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
      361 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
      421 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
      481 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
      541 ttttgcggcc gcaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
      601 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
      661 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
      721 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
      781 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
      841 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
      901 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
      961 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     1021 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     1081 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     1141 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     1201 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     1261 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     1321 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     1381 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     1441 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     1501 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     1561 acatttcccc gaaaagtgcc acctgcagcc tgaatatggg ccaaacagga tatctgtggt
     1621 aagcagttcc tgccccggct cagggccaag aacagatgga acagctgaat atgggccaaa
     1681 caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggtcccca
     1741 gatgcggtcc agccctcagc agtttctaga gaaccatcag atgtttccag ggtgccccaa
     1801 ggacctgaaa tgaccctgtg ccttatttga actaaccaat cagttcgctt ctcgcttctg
     1861 ttcgcgcgct tctgctcccc gagctcaata aaagagccca caacccctca ctcggggcgc
     1921 cagtcctccg attgactgag tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt
     1981 tgcatccgac ttgtggtctc gctgttcctt gggagggtct cctctgagtg attgactacc
     2041 cgtcagcggg ggtctttcat ttgggggctc gtccgggatc gggagacccc tgcccaggga
     2101 ccaccgaccc accaccggga ggcaagctgg ccagcaactt atctgtgtct gtccgattgt
     2161 ctagtgtcta tgactgattt tatgcgcctg cgtcggtact agttagctaa ctagctctgt
     2221 atctggcgga cccgtggtgg aactgacgag ttctgaacac ccggccgcaa ccctgggaga
     2281 cgtcccaggg actttggggg ccgtttttgt ggcccgacct gaggaaggga gtcgatgtgg
     2341 aatccgaccc cgtcaggata tgtggttctg gtaggagacg agaacctaaa acagttcccg
     2401 cctccgtctg aatttttgct ttcggtttgg aaccgaagcc gcgcgtcttg tctgctgcag
     2461 cgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt gtctgaaaat
     2521 tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa agatgtcgag
     2581 cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac cttctgctct
     2641 gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa ccgagacctc
     2701 atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc agaccaggtc
     2761 ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt caagcccttt
     2821 gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc ccttgaacct
     2881 cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc tctaggcgcc
     2941 ggccggatcc cagtgtggtg gtacgtagga attcgccagc acagtggtcg agatccattg
     3001 tgctctaaag ccagcctctg ccaggttcgg tccgccatcc tcgtcccgtc ctccgccggc
     3061 ccctgccccg cgcccaggga tcctccagct cctttcgccc gcgccctccg ttcgctccgg
     3121 acaccatgga caagttttgg tggcacgcag cctggggact ctgcctcgtg ccgctgagcc
     3181 tggcgcagat cgatttgaat ataacctgcc gctttgcagg tgtattccac gtggagaaaa
     3241 atggtcgcta cagcatctct cggacggagg ccgctgacct ctgcaaggct ttcaatagca
     3301 ccttgcccac aatggcccag atggagaaag ctctgagcat cggatttgag acctgcaggt
     3361 atgggttcat agaagggcat gtggtgattc cccggatcca ccccaactcc atctgtgcag
     3421 caaacaacac aggggtgtac atcctcacat acaacacctc ccagtatgac acatattgct
     3481 tcaatgcttc agctccacct gaagaagatt gtacatcagt cacagacctg cccaatgcct
     3541 ttgatggacc aattaccata actattgtta accgtgatgg cacccgctat gtccagaaag
     3601 gagaatacag aacgaatcct gaagacatct accccagcaa ccctactgat gatgacgtga
     3661 gcagcggctc ctccagtgaa aggagcagca cttcaggagg ttacatcttt tacacctttt
     3721 ctactgtaca ccccatccca gacgaagaca gtccctggat caccgacagc acagacagaa
     3781 tccctgctac cagagaccaa gacacattcc accccagtgg ggggtcccat accactcatg
     3841 aatctgaatc agatggacac tcacatggga gtcaagaagg tggagcaaac acaacctctg
     3901 gtcctataag gacaccccaa attccagaat ggctgatcat cttggcatcc ctcttggcct
     3961 tggctttgat tcttgcagtt tgcattgcag tcaacagtcg aagaaggtgt gggcagaaga
     4021 aaaagctagt gatcaacagt ggcaatggag ctgtggagga cagaaagcca agtggactca
     4081 acggagaggc cagcaagtct caggaaatgg tgcatttggt gaacaaggag tcgtcagaaa
     4141 ctccagacca gtttatgaca gctgatgaga caaggaacct gcagaatgtg gacatgaaga
     4201 ttggggtgta acacctacac cattatcttg gaaagaaaca accgttgtaa acataaccat
     4261 tacagggagc tgggacactt aacagatgca atgtgctact gattgtttca ttgcgaatct
     4321 tttttagcat aaaattttct actctttttg ttaaaaaaaa aaaactttag agcacaatgg
     4381 atctcgaccc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     4441 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     4501 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     4561 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     4621 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     4681 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gcttaccatg
     4741 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
     4801 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
     4861 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     4921 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
     4981 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     5041 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     5101 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
     5161 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     5221 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     5281 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgc
     5341 ccgccccacg acccgcagcg cccgaccgaa aggagcgcac gaccccatgc atcgataaaa
     5401 taaaagattt tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt
     5461 ggcaagctag cttaagtaac gccattttgc aaggcatgga aaaatacata actgagaata
     5521 gagaagttca gatcaaggtc aggaacagat ggaacagctg aatatgggcc aaacaggata
     5581 tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggaac agctgaatat
     5641 gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat
     5701 ggtccccaga tgcggtccag ccctcagcag tttctagaga accatcagat gtttccaggg
     5761 tgccccaagg acctgaaatg accctgtgcc ttatttgaac taaccaatca gttcgcttct
     5821 cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa agagcccaca acccctcact
     5881 cggggcgcca gtcctccgat tgactgagtc gcccgggtac ccgtgtatcc aataaaccct
     5941 cttgcagttg catccgactt gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
     6001 tgactacccg tcagcggggg tctttcacat gcagcatgta tcaaaattaa tttggttttt
     6061 tttcttaagt atttacatta aatggccata gttgcattaa tgaatcggcc aacgcgcggg
     6121 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     6181 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     6241 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     6301 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     6361 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     6421 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     6481 cc
//
