LOCUS       sequence-190675-        1040 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  in vitro pre-mRNA splicing substrate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Moore MJ, Sharp PA
  TITLE     Site-specific modification of pre-mRNA: the 2'-hydroxyl groups at 
            the splice sites.
  JOURNAL   Science. 1992 May 15;256(5059):992-7.
  PUBMED    1589782
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "1992-05-15"; volume: "256(5059)"; pages: "992-7"
COMMENT     Sequence Label: pip85.A
FEATURES             Location/Qualifiers
     source          1..1040
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(243..262)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(244..262)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(269..286)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(269..285)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(278..300)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(426..881)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(563..584)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     775..794
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(948..967)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gggaacaaaa gcttgcatgc agagaccgcg aagagtttgt cctgcagtat gttgtgtagg
       61 acctgaggga aaaagaagaa ggggcccagt tagtacgccc tcgagacgag ctgacatcgt
      121 cgacggaaac cctcggaaac cctggactac tctagaagtc atgcccgctt ttagagggat
      181 ccatactcac ctggtagggt acctcgcaga cagcgatgcg gaagagagtg agctcgaatt
      241 cgccctatag tgagtcgtat tacaattcac tggccgtcgt tttacaacgt cgtgactggg
      301 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
      361 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
      421 aatggaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
      481 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
      541 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
      601 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
      661 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
      721 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
      781 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
      841 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcctgatgcg gtattttctc
      901 cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct
      961 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     1021 gcttgtctgc tcccggcatc
//
