LOCUS       sequence-190702-        6218 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent protein for superresolution imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6218)
  AUTHORS   Wang S, Moffitt JR, Dempsey GT, Xie XS, Zhuang X
  TITLE     Characterization and development of photoactivatable fluorescent 
            proteins for single-molecule-based superresolution imaging.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Jun 10;111(23):8452-7. doi: 
            10.1073/pnas.1406593111. Epub 2014 May 27.
  PUBMED    24912163
REFERENCE   2  (bases 1 to 6218)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1406593111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-06-10- 10"; volume: "111"; issue: "23"; pages: "8452-7"
COMMENT     Sequence Label: pmMaple3-CAM
FEATURES             Location/Qualifiers
     source          1..6218
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(96..974)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1001..1285
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1208..1227
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     protein_bind    2055..2088
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     promoter        2148..2250
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(2317..2336)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     protein_bind    2899..2932
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(3001..3018)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      3170..3256
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3348..3375
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3394..3485
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3486..3554,3555..4346)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3704..3723)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4388..4843
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4475..4494)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      4954..5542
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5443..5462
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5696..5713
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5728..5868
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5794..5813)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5954..5976
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6115..6134
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 atcgatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac
       61 tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca
      121 ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta
      181 aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata
      241 ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag
      301 cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag
      361 caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg
      421 tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct
      481 tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc
      541 ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc
      601 gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca
      661 tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga
      721 tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa
      781 acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata
      841 taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc
      901 ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt
      961 tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat
     1021 tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta
     1081 accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt
     1141 aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca
     1201 ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta
     1261 tcgcaactct ctactgtttc tccatacccg tttttttggg ctagcaggag gaattcacca
     1321 tgtctggtgg cggcggttct aaagttagca agggcgaaga aaccatcatg agcgtgatca
     1381 agccggacat gaagatcaag ctgcgcatgg agggcaacgt gaacggccat gcctttgtga
     1441 tcgagggcga gggcagcggt aagccgtttg agggcatcca gaccatcgac ctggaggtta
     1501 aggaaggcgc accgctgccg tttgcctacg acatcctgac caccgcattc cactacggca
     1561 accgcgtgtt caccaagtac ccgcgcaaga tcccggacta cttcaagcag agcttcccgg
     1621 agggctacag ttgggaacgc agcatgacct acgaggacgg cggtatctgc aacgccacca
     1681 acgacatcac catggaagaa gatagcttca tcaacaagat ccacttcaag ggcacaaact
     1741 tcccgccgaa tggtccggtt atgcagaagc gcaccgttgg ctgggaggtg agcaccgaga
     1801 agatgtatgt gcgcgacggt gtgctgaagg gcgacgtgaa gatgaagctg ctgctgaagg
     1861 gtggcagcca ctaccgctgc gacttccgca ccacctacaa agttaagcaa aaggcagtga
     1921 agttaccgaa ggcccacttc gtggaccacc gcatcgaaat cctgagccac gacaaggact
     1981 ataacaaagt gaagctgtac gagcacgccg tggcccgtaa cagcaccgac agcatggatg
     2041 agctgtacaa ataagaagtt cctattctct agaaagtata ggaacttcac tggtgtccct
     2101 gttgataccg ggaagccctg ggccaacttt tggcgaaaat gagacgttga tcggcacgta
     2161 agaggttcca actttcacca taatgaaata agatcactac cgggcgtatt ttttgagtta
     2221 tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc
     2281 gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa
     2341 tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa
     2401 aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat
     2461 ccggaattcc gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct
     2521 tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac
     2581 gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac
     2641 ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg
     2701 gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt
     2761 ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag
     2821 gttcatcatg ccgtctgtga tggcttccat gtcggcagaa tgcttaatga attacaacag
     2881 tactgcgatg agtggcagga agttcctatt ctctagaaag tataggaact tcgaagcagc
     2941 tccagcctac aatttaataa gaaagcttgg ctgttttggc ggatgagaga agattttcag
     3001 cctgatacag attaaatcag aacgcagaag cggtctgata aaacagaatt tgcctggcgg
     3061 cagtagcgcg gtggtcccac ctgaccccat gccgaactca gaagtgaaac gccgtagcgc
     3121 cgatggtagt gtgggtctcc ccatgcgaga gtagggaact gccaggcatc aaataaaacg
     3181 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     3241 cctgagtagg acaaatccgc cgggagcgga tttgaacgtt gcgaagcaac ggcccggagg
     3301 gtggcgggca ggacgcccgc cataaactgc caggcatcaa attaagcaga aggccatcct
     3361 gacggatggc ctttttgcgt ttctacaaac tcttttgttt atttttctaa atacattcaa
     3421 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     3481 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     3541 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     3601 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     3661 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     3721 tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     3781 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     3841 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     3901 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     3961 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     4021 cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     4081 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     4141 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     4201 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     4261 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     4321 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     4381 ttgatttacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     4441 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     4501 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     4561 cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt
     4621 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     4681 aatagtggac tcttgttcca aacttgaaca acactcaacc ctatctcggg ctattctttt
     4741 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     4801 aaatttaacg cgaattttaa caaaatatta acgtttacaa tttaaaagga tctaggtgaa
     4861 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     4921 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     4981 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     5041 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     5101 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     5161 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     5221 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     5281 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     5341 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     5401 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     5461 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     5521 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     5581 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     5641 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     5701 gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg
     5761 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     5821 aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg
     5881 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     5941 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     6001 gcgaggcagc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc
     6061 cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat
     6121 gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg
     6181 tccggcgtag aggatctgct catgtttgac agcttatc
//
