LOCUS       sequence-190703-       10657 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for constitutive expression of human mutant PC5A 
            (T288P) with C-terminal V5 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10657)
  AUTHORS   Bajikar SS, Wang CC, Borten MA, Pereira EJ, Atkins KA, Janes KA
  TITLE     Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor 
            Sequestration in Triple-Negative Breast Cancer.
  JOURNAL   Dev Cell. 2017 Nov 20;43(4):418-435.e13. doi: 
            10.1016/j.devcel.2017.10.027.
  PUBMED    29161592
REFERENCE   2  (bases 1 to 10657)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2017.10"; journalName: "Dev Cell"; date: 
            "2017-11-20- 20"; volume: "43"; issue: "4"; pages: "418-435.e13"
COMMENT     Sequence Label: pLX302 PCSK5 (T288P)-V5 puro
FEATURES             Location/Qualifiers
     source          1..10657
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     212..232
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    357..474
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        591..894
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        895..1098
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1049..1069
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    1121..1145
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3885..3909)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3911..3952
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3994..4582
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4047..4067)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4465..4476)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4654..4887
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4965..5086
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5002..5021)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5056..5075
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5126..5261
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5281..5300)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5282..5300)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5310..5327)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5310..5326)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5319..5341)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5468..5923
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5555..5574)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5765..5786
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5949..6053
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6054..6122,6123..6914)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6272..6291)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7085..7673
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7574..7593
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7827..7844
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7961..7982
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7997..8002,8003..8020,8021..8027)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8035..8051
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8040..8062
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8059..8075
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8059..8075
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8094..8114
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8096..8114
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8142..8368
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8369..8549
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8596..8721
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9214..9447
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9632..9676
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9851..10351
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     10270..10288
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             join(10373..10657,1..315)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(10373..10392)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
       61 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
      121 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
      181 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
      241 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
      301 aagcccggtg cctgagaatt ctagatcttg agacaaatgg cagtattcat ccacaatttt
      361 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
      421 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt
      481 tattacaggg acagcagaga tccactttgg cgccggctcg agggggcccg gggttattaa
      541 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
      601 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
      661 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
      721 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
      781 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
      841 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
      901 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
      961 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
     1021 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
     1081 gtctatataa gcagagctct ctggctaact gtcgggatca acaagtttgt acaaaaaagt
     1141 tggcatgggc tgggggagcc gctgctgctg cccgggacgt ttggacctgc tgtgcgtgct
     1201 ggcgctgctc gggggctgcc tgctccccgt gtgtcggacg cgcgtctaca ccaaccactg
     1261 ggcagtcaaa atcgccgggg gcttcccgga ggccaaccgt atcgccagca agtacggatt
     1321 catcaacata ggacagatag gggccctgaa ggactactac cacttctacc atagcaggac
     1381 gattaaaagg tcagttatct cgagcagagg gacccacagt ttcatttcaa tggaaccaaa
     1441 ggtggaatgg atccaacagc aagtggtaaa aaagcggaca aagagggatt atgacttcag
     1501 tcgtgcccag tctacctatt tcaatgatcc caagtggccc agcatgtggt atatgcactg
     1561 cagtgacaat acacatccct gccagtctga catgaatatc gaaggagcct ggaagagagg
     1621 ctacacggga aagaacattg tggtcactat cctggatgac ggaattgaga gaacccatcc
     1681 agatctgatg caaaactacg atgctctggc aagttgcgac gtgaatggga atgacttgga
     1741 cccaatgcct cgttatgatg caagcaacga gaacaagcat gggactcgct gtgctggaga
     1801 agtggcagcc gctgcaaaca attcgcactg cacagtcgga attgctttca acgccaagat
     1861 cggaggagtg cgaatgctgg acggagatgt cacggacatg gttgaagcaa aatcagttag
     1921 cttcaacccc cagcacgtgc acatttacag cgccagctgg ggcccggatg atgatggcaa
     1981 gactgtggac ggaccagccc ccctcccccg gcaagccttt gaaaacggcg ttagaatggg
     2041 gcggagaggc ctcggctctg tgtttgtttg ggcatctgga aatggtggaa ggagcaaaga
     2101 ccactgctcc tgtgatggct acaccaacag catctacacc atctccatca gcagcactgc
     2161 agaaagcgga aagaaacctt ggtacctgga agagtgttca tccacgctgg ccacaaccta
     2221 cagcagcggg gagtcctacg ataagaaaat catcactaca gatctgaggc agcgttgcac
     2281 ggacaaccac actgggacgt cagcctcagc ccccatggct gcaggcatca ttgcgctggc
     2341 cctggaagcc aatccgtttc tgacctggag agacgtacag catgttattg tcaggacttc
     2401 ccgtgcggga catttgaacg ctaatgactg gaaaaccaat gctgctggtt ttaaggtgag
     2461 ccatctttat ggatttggac tgatggacgc agaagccatg gtgatggagg cagagaagtg
     2521 gaccaccgtt ccccggcagc acgtgtgtgt ggagagcaca gaccgacaaa tcaagacaat
     2581 ccgccctaac agtgcagtgc gctccatcta caaagcttca ggctgctcgg ataaccccaa
     2641 ccgccatgtc aactacctgg agcacgtcgt tgtgcgcatc accatcaccc accccaggag
     2701 aggagacctg gccatctacc tgacctcgcc ctctggaact aggtctcagc ttttggccaa
     2761 caggctattt gatcactcca tggaaggatt caaaaactgg gagttcatga ccattcattg
     2821 ctggggagaa agagctgctg gtgactgggt ccttgaagtt tatgatactc cctctcagct
     2881 aaggaacttt aagactccag gtaaattgaa agaatggtct ttggtcctct acggcacctc
     2941 cgtgcagcca tattcaccaa ccaatgaatt tccgaaagtg gaacggttcc gctatagccg
     3001 agttgaagac cccacagacg actatggcac agaggattat gcaggtccct gcgaccctga
     3061 gtgcagtgag gttggctgtg acgggccagg accagaccac tgcaatgact gtttgcacta
     3121 ctactacaag ctgaaaaaca ataccaggat ctgtgtctcc agctgccccc ctggccacta
     3181 ccacgccgac aagaagcgct gcaggaagtg tgcccccaac tgtgagtcct gctttgggag
     3241 ccatggtgac caatgcatgt cctgcaaata tggatacttt ctgaatgaag aaaccaacag
     3301 ctgtgttact cactgccctg atgggtcata tcaggatacc aagaaaaatc tttgccggaa
     3361 atgcagtgaa aactgcaaga catgtactga attccataac tgtacagaat gtagggatgg
     3421 gttaagcctg cagggatccc ggtgctctgt ctcctgtgaa gatggacggt atttcaacgg
     3481 ccaggactgc cagccctgcc accgcttctg cgccacttgt gctggggcag gagctgatgg
     3541 gtgcattaac tgcacagagg gctacttcat ggaggatggg agatgcgtgc agagctgtag
     3601 tatcagctat tactttgacc actcttcaga gaatggatac aaatcctgca aaaaatgtga
     3661 tatcagttgt ttgacgtgca atggcccagg attcaagaac tgtacaagct gccctagtgg
     3721 gtatctctta gacttaggaa tgtgtcaaat gggagccatt tgcaaggatg caacggaaga
     3781 gtcctgggcg gaaggaggct tctgtatgct tgtgaaaaag aacaatctgt gccaacggaa
     3841 ggttcttcaa caactttgct gcaaaacatg tacatttcaa ggctgcccaa ctttcttgta
     3901 caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgtagtaatg
     3961 agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta caaaatttgt
     4021 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     4081 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     4141 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     4201 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     4261 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     4321 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     4381 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     4441 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     4501 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     4561 tccctttggg ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag
     4621 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     4681 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     4741 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     4801 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     4861 tttagtcagt gtggaaaatc tctagcagta cgtatagtag ttcatgtcat cttattattc
     4921 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     4981 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     5041 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     5101 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     5161 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     5221 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
     5281 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     5341 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     5401 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     5461 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     5521 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     5581 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     5641 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     5701 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     5761 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     5821 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     5881 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
     5941 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     6001 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     6061 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     6121 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     6181 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     6241 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     6301 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     6361 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     6421 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     6481 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     6541 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     6601 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     6661 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     6721 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     6781 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     6841 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     6901 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     6961 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     7021 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     7081 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     7141 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     7201 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     7261 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     7321 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     7381 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     7441 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     7501 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     7561 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     7621 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     7681 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     7741 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     7801 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     7861 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     7921 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     7981 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     8041 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
     8101 accctcacta aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact
     8161 cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa
     8221 gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca
     8281 acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt
     8341 atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg
     8401 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     8461 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     8521 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
     8581 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
     8641 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
     8701 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
     8761 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
     8821 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
     8881 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
     8941 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
     9001 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
     9061 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
     9121 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
     9181 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
     9241 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
     9301 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
     9361 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
     9421 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
     9481 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
     9541 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     9601 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
     9661 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
     9721 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
     9781 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
     9841 gaggggaccc ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc
     9901 tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc
     9961 cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc
    10021 tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac
    10081 ggaagccgca cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc
    10141 gcgccgaccg cgatgggctg tggccaatag cggctgctga gcagggcgcg ccgagagcag
    10201 cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct
    10261 gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct
    10321 cgttgaccga atcaccgacc tctctcccca gggggtacca ccggagctta ccatgaccga
    10381 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
    10441 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
    10501 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
    10561 ggtgtgggtc gcggacgacg gcgccgccgt ggcggtctgg accacgccgg agagcgtcga
    10621 agcgggggcg gtgttcgccg agatcggccc gcgcatg
//
