LOCUS       sequence-190706-       10657 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for constitutive expression of human PCSK5 with 
            C-terminal V5 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10657)
  AUTHORS   Bajikar SS, Wang CC, Borten MA, Pereira EJ, Atkins KA, Janes KA
  TITLE     Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor 
            Sequestration in Triple-Negative Breast Cancer.
  JOURNAL   Dev Cell. 2017 Nov 20;43(4):418-435.e13. doi: 
            10.1016/j.devcel.2017.10.027.
  PUBMED    29161592
REFERENCE   2  (bases 1 to 10657)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2017.10"; journalName: "Dev Cell"; date: 
            "2017-11-20- 20"; volume: "43"; issue: "4"; pages: "418-435.e13"
COMMENT     Sequence Label: pLX302 PCSK5-V5 puro
FEATURES             Location/Qualifiers
     source          1..10657
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     100..117
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    234..255
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(270..275,276..293,294..300)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    308..324
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     313..335
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     332..348
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     332..348
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     367..387
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        369..387
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        415..641
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             642..822
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    869..994
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1487..1720
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1905..1949
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2124..2624
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2543..2561
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2646..3245
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2646..2665)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3142..3162
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3287..3404
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3521..3824
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3825..4028
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3979..3999
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    4051..4075
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6815..6839)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6841..6882
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6924..7512
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6977..6997)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7395..7406)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7584..7817
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7895..8016
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7932..7951)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7986..8005
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8056..8191
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8211..8230)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8212..8230)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8240..8257)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8240..8256)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8249..8271)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8398..8853
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8485..8504)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8695..8716
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8879..8983
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8984..9052,9053..9844)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9202..9221)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10015..10603
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10504..10523
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
       61 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
      121 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
      181 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
      241 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
      301 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
      361 aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt
      421 cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac
      481 aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct
      541 tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt
      601 gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc
      661 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
      721 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
      781 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
      841 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
      901 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
      961 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
     1021 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
     1081 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
     1141 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     1201 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     1261 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
     1321 cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     1381 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     1441 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     1501 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     1561 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     1621 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     1681 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     1741 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     1801 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     1861 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     1921 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     1981 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     2041 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     2101 acctcccaac cccgagggga cccttgcgcc ttttccaagg cagccctggg tttgcgcagg
     2161 gacgcggctg ctctgggcgt ggttccggga aacgcagcgg cgccgaccct gggtctcgca
     2221 cattcttcac gtccgttcgc agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc
     2281 cggcgacgct tcctgctccg cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc
     2341 ggacgtgaca aacggaagcc gcacgtctca ctagtaccct cgcagacgga cagcgccagg
     2401 gagcaatggc agcgcgccga ccgcgatggg ctgtggccaa tagcggctgc tcagcagggc
     2461 gcgccgagag cagcggccgg gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt
     2521 gggccctgtt cctgcccgcg cggtgttccg cattctgcaa gcctccggag cgcacgtcgg
     2581 cagtcggctc cctcgttgac cgaatcaccg acctctctcc ccagggggta ccaccggagc
     2641 ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg
     2701 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc
     2761 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
     2821 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc cgtggcggtc tggaccacgc
     2881 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
     2941 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
     3001 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
     3061 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
     3121 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
     3181 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
     3241 cctgagaatt ctagatcttg agacaaatgg cagtattcat ccacaatttt aaaagaaaag
     3301 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     3361 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     3421 acagcagaga tccactttgg cgccggctcg agggggcccg gggttattaa tagtaatcaa
     3481 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     3541 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     3601 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     3661 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     3721 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     3781 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     3841 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     3901 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     3961 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     4021 gcagagctct ctggctaact gtcgggatca acaagtttgt acaaaaaagt tggcatgggc
     4081 tgggggagcc gctgctgctg cccgggacgt ttggacctgc tgtgcgtgct ggcgctgctc
     4141 gggggctgcc tgctccccgt gtgtcggacg cgcgtctaca ccaaccactg ggcagtcaaa
     4201 atcgccgggg gcttcccgga ggccaaccgt atcgccagca agtacggatt catcaacata
     4261 ggacagatag gggccctgaa ggactactac cacttctacc atagcaggac gattaaaagg
     4321 tcagttatct cgagcagagg gacccacagt ttcatttcaa tggaaccaaa ggtggaatgg
     4381 atccaacagc aagtggtaaa aaagcggaca aagagggatt atgacttcag tcgtgcccag
     4441 tctacctatt tcaatgatcc caagtggccc agcatgtggt atatgcactg cagtgacaat
     4501 acacatccct gccagtctga catgaatatc gaaggagcct ggaagagagg ctacacggga
     4561 aagaacattg tggtcactat cctggatgac ggaattgaga gaacccatcc agatctgatg
     4621 caaaactacg atgctctggc aagttgcgac gtgaatggga atgacttgga cccaatgcct
     4681 cgttatgatg caagcaacga gaacaagcat gggactcgct gtgctggaga agtggcagcc
     4741 gctgcaaaca attcgcactg cacagtcgga attgctttca acgccaagat cggaggagtg
     4801 cgaatgctgg acggagatgt cacggacatg gttgaagcaa aatcagttag cttcaacccc
     4861 cagcacgtgc acatttacag cgccagctgg ggcccggatg atgatggcaa gactgtggac
     4921 ggaccagccc ccctcacccg gcaagccttt gaaaacggcg ttagaatggg gcggagaggc
     4981 ctcggctctg tgtttgtttg ggcatctgga aatggtggaa ggagcaaaga ccactgctcc
     5041 tgtgatggct acaccaacag catctacacc atctccatca gcagcactgc agaaagcgga
     5101 aagaaacctt ggtacctgga agagtgttca tccacgctgg ccacaaccta cagcagcggg
     5161 gagtcctacg ataagaaaat catcactaca gatctgaggc agcgttgcac ggacaaccac
     5221 actgggacgt cagcctcagc ccccatggct gcaggcatca ttgcgctggc cctggaagcc
     5281 aatccgtttc tgacctggag agacgtacag catgttattg tcaggacttc ccgtgcggga
     5341 catttgaacg ctaatgactg gaaaaccaat gctgctggtt ttaaggtgag ccatctttat
     5401 ggatttggac tgatggacgc agaagccatg gtgatggagg cagagaagtg gaccaccgtt
     5461 ccccggcagc acgtgtgtgt ggagagcaca gaccgacaaa tcaagacaat ccgccctaac
     5521 agtgcagtgc gctccatcta caaagcttca ggctgctcgg ataaccccaa ccgccatgtc
     5581 aactacctgg agcacgtcgt tgtgcgcatc accatcaccc accccaggag aggagacctg
     5641 gccatctacc tgacctcgcc ctctggaact aggtctcagc ttttggccaa caggctattt
     5701 gatcactcca tggaaggatt caaaaactgg gagttcatga ccattcattg ctggggagaa
     5761 agagctgctg gtgactgggt ccttgaagtt tatgatactc cctctcagct aaggaacttt
     5821 aagactccag gtaaattgaa agaatggtct ttggtcctct acggcacctc cgtgcagcca
     5881 tattcaccaa ccaatgaatt tccgaaagtg gaacggttcc gctatagccg agttgaagac
     5941 cccacagacg actatggcac agaggattat gcaggtccct gcgaccctga gtgcagtgag
     6001 gttggctgtg acgggccagg accagaccac tgcaatgact gtttgcacta ctactacaag
     6061 ctgaaaaaca ataccaggat ctgtgtctcc agctgccccc ctggccacta ccacgccgac
     6121 aagaagcgct gcaggaagtg tgcccccaac tgtgagtcct gctttgggag ccatggtgac
     6181 caatgcatgt cctgcaaata tggatacttt ctgaatgaag aaaccaacag ctgtgttact
     6241 cactgccctg atgggtcata tcaggatacc aagaaaaatc tttgccggaa atgcagtgaa
     6301 aactgcaaga catgtactga attccataac tgtacagaat gtagggatgg gttaagcctg
     6361 cagggatccc ggtgctctgt ctcctgtgaa gatggacggt atttcaacgg ccaggactgc
     6421 cagccctgcc accgcttctg cgccacttgt gctggggcag gagctgatgg gtgcattaac
     6481 tgcacagagg gctacttcat ggaggatggg agatgcgtgc agagctgtag tatcagctat
     6541 tactttgacc actcttcaga gaatggatac aaatcctgca aaaaatgtga tatcagttgt
     6601 ttgacgtgca atggcccagg attcaagaac tgtacaagct gccctagtgg gtatctctta
     6661 gacttaggaa tgtgtcaaat gggagccatt tgcaaggatg caacggaaga gtcctgggcg
     6721 gaaggaggct tctgtatgct tgtgaaaaag aacaatctgt gccaacggaa ggttcttcaa
     6781 caactttgct gcaaaacatg tacatttcaa ggctgcccaa ctttcttgta caaagtggtt
     6841 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct
     6901 aaccggtggc gcgttaagtc gacaatcaac ctctggatta caaaatttgt gaaagattga
     6961 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     7021 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     7081 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     7141 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     7201 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     7261 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     7321 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     7381 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     7441 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     7501 ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag atcttagcca
     7561 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     7621 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     7681 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     7741 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     7801 gtggaaaatc tctagcagta cgtatagtag ttcatgtcat cttattattc agtatttata
     7861 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
     7921 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     7981 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg
     8041 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     8101 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     8161 cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg
     8221 agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     8281 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     8341 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg
     8401 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     8461 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     8521 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     8581 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     8641 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     8701 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     8761 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     8821 cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg
     8881 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     8941 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     9001 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     9061 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     9121 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     9181 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     9241 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     9301 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     9361 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     9421 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     9481 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     9541 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     9601 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     9661 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     9721 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     9781 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     9841 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     9901 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     9961 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
    10021 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
    10081 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
    10141 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
    10201 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
    10261 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
    10321 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
    10381 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
    10441 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
    10501 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
    10561 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
    10621 ctttttacgg ttcctggcct tttgctggcc ttttgct
//
