LOCUS       sequence-190708-       11125 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for constitutive expression of human LacZ with 
            C-terminal V5 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11125)
  AUTHORS   Bajikar SS, Wang CC, Borten MA, Pereira EJ, Atkins KA, Janes KA
  TITLE     Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor 
            Sequestration in Triple-Negative Breast Cancer.
  JOURNAL   Dev Cell. 2017 Nov 20;43(4):418-435.e13. doi: 
            10.1016/j.devcel.2017.10.027.
  PUBMED    29161592
REFERENCE   2  (bases 1 to 11125)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2017.10"; journalName: "Dev Cell"; date: 
            "2017-11-20- 20"; volume: "43"; issue: "4"; pages: "418-435.e13"
COMMENT     Sequence Label: pLX302 LacZ-V5 puro
FEATURES             Location/Qualifiers
     source          1..11125
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     267..287
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    412..529
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        646..949
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        950..1153
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1104..1124
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    1176..1200
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1215..4259
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAELQLSAGRYHYQLVWCQK"
     protein_bind    complement(4279..4303)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4356..4397
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    complement(4408..4432)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4434..4475
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4517..5105
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4570..4590)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4988..4999)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5177..5410
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5488..5609
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5525..5544)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5579..5598
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5649..5784
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5804..5823)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5805..5823)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5833..5850)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5833..5849)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      5991..6446
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6078..6097)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6288..6309
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6472..6576
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6577..6645,6646..7437)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6795..6814)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7608..8196
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8097..8116
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8350..8367
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8484..8505
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8520..8525,8526..8543,8544..8550)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8558..8574
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8563..8585
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8582..8598
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8582..8598
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8617..8637
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8619..8637
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8665..8891
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8892..9072
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9119..9244
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9737..9970
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10155..10199
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        10374..10874
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     10793..10811
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             join(10896..11125,1..370)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(10896..10915)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
       61 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
      121 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
      181 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
      241 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
      301 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
      361 cggtgcctga gaattctaga tcttgagaca aatggcagta ttcatccaca attttaaaag
      421 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
      481 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
      541 cagggacagc agagatccac tttggcgccg gctcgagggg gcccggggtt attaatagta
      601 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
      661 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
      721 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
      781 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
      841 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
      901 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
      961 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     1021 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     1081 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     1141 tataagcaga gctctctggc taactgtcgg gatcaacaag tttgtacaaa aaagttggca
     1201 ccatgataga tcccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     1261 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     1321 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcttt gcctggtttc
     1381 cggcaccaga agcggtgccg gaaagctggc tggagtgcga tcttcctgag gccgatactg
     1441 tcgtcgtccc ctcaaactgg cagatgcacg gttacgatgc gcccatctac accaacgtga
     1501 cctatcccat tacggtcaat ccgccgtttg ttcccacgga gaatccgacg ggttgttact
     1561 cgctcacatt taatgttgat gaaagctggc tacaggaagg ccagacgcga attatttttg
     1621 atggcgttaa ctcggcgttt catctgtggt gcaacgggcg ctgggtcggt tacggccagg
     1681 acagtcgttt gccgtctgaa tttgacctga gcgcattttt acgcgccgga gaaaaccgcc
     1741 tcgcggtgat ggtgctgcgc tggagtgacg gcagttatct ggaagatcag gatatgtggc
     1801 ggatgagcgg cattttccgt gacgtctcgt tgctgcataa accgactaca caaatcagcg
     1861 atttccatgt tgccactcgc tttaatgatg atttcagccg cgctgtactg gaggctgaag
     1921 ttcagatgtg cggcgagttg cgtgactacc tacgggtaac agtttcttta tggcagggtg
     1981 aaacgcaggt cgccagcggc accgcgcctt tcggcggtga aattatcgat gagcgtggtg
     2041 gttatgccga tcgcgtcaca ctacgtctga acgtcgaaaa cccgaaactg tggagcgccg
     2101 aaatcccgaa tctctatcgt gcggtggttg aactgcacac cgccgacggc acgctgattg
     2161 aagcagaagc ctgcgatgtc ggtttccgcg aggtgcggat tgaaaatggt ctgctgctgc
     2221 tgaacggcaa gccgttgctg attcgaggcg ttaaccgtca cgagcatcat cctctgcatg
     2281 gtcaggtcat ggatgagcag acgatggtgc aggatatcct gctgatgaag cagaacaact
     2341 ttaacgccgt gcgctgttcg cattatccga accatccgct gtggtacacg ctgtgcgacc
     2401 gctacggcct gtatgtggtg gatgaagcca atattgaaac ccacggcatg gtgccaatga
     2461 atcgtctgac cgatgatccg cgctggctac cggcgatgag cgaacgcgta acgcgaatgg
     2521 tgcagcgcga tcgtaatcac ccgagtgtga tcatctggtc gctggggaat gaatcaggcc
     2581 acggcgctaa tcacgacgcg ctgtatcgct ggatcaaatc tgtcgatcct tcccgcccgg
     2641 tgcagtatga aggcggcgga gccgacacca cggccaccga tattatttgc ccgatgtacg
     2701 cgcgcgtgga tgaagaccag cccttcccgg ctgtgccgaa atggtccatc aaaaaatggc
     2761 tttcgctacc tggagagacg cgcccgctga tcctttgcga atacgcccac gcgatgggta
     2821 acagtcttgg cggtttcgct aaatactggc aggcgtttcg tcagtatccc cgtttacagg
     2881 gcggcttcgt ctgggactgg gtggatcagt cgctgattaa atatgatgaa aacggcaacc
     2941 cgtggtcggc ttacggcggt gattttggcg atacgccgaa cgatcgccag ttctgtatga
     3001 acggtctggt ctttgccgac cgcacgccgc atccagcgct gacggaagca aaacaccagc
     3061 agcagttttt ccagttccgt ttatccgggc aaaccatcga agtgaccagc gaatacctgt
     3121 tccgtcatag cgataacgag ctcctgcact ggatggtggc gctggatggt aagccgctgg
     3181 caagcggtga agtgcctctg gatgtcgctc cacaaggtaa acagttgatt gaactgcctg
     3241 aactaccgca gccggagagc gccgggcaac tctggctcac agtacgcgta gtgcaaccga
     3301 acgcgaccgc atggtcagaa gccggccaca tcagcgcctg gcagcagtgg cgtctggcgg
     3361 aaaacctcag tgtgacgctc cccgccgcgt cccacgccat cccgcatctg accaccagcg
     3421 aaatggattt ttgcatcgag ctgggtaata agcgttggca atttaaccgc cagtcaggct
     3481 ttctttcaca gatgtggatt ggcgataaaa aacaactgct gacgccgctg cgcgatcagt
     3541 tcacccgtgc accgctggat aacgacattg gcgtaagtga agcgacccgc attgacccta
     3601 acgcctgggt cgaacgctgg aaggcggcgg gccattacca ggccgaagca gcgttgttgc
     3661 agtgcacggc agatacactt gctgacgcgg tgctgattac gaccgctcac gcgtggcagc
     3721 atcaggggaa aaccttattt atcagccgga aaacctaccg gattgatggt agtggtcaaa
     3781 tggcgattac cgttgatgtt gaagtggcga gcgatacacc gcatccggcg cggattggcc
     3841 tgaactgcca gctggcgcag gtagcagagc gggtaaactg gctcggatta gggccgcaag
     3901 aaaactatcc cgaccgcctt actgccgcct gttttgaccg ctgggatctg ccattgtcag
     3961 acatgtatac cccgtacgtc ttcccgagcg aaaacggtct gcgctgcggg acgcgcgaat
     4021 tgaattatgg cccacaccag tggcgcggcg acttccagtt caacatcagc cgctacagtc
     4081 aacagcaact gatggaaacc agccatcgcc atctgctgca cgcggaagaa ggcacatggc
     4141 tgaatatcga cggtttccat atggggattg gtggagacga ctcctggagc ccgtcagtat
     4201 cggcggaatt acagctgagc gccggtcgct accattacca gttggtctgg tgtcaaaaaa
     4261 ctaagggtgg gcgcgccgac ccagctttct tgtacaaagt ggttgatatc cagcacagtg
     4321 gcggccgctc gagtctagag ggcccgcggt tcgaaggtaa gcctatccct aaccctctcc
     4381 tcggtctcga ttctacgcgt accggtttgc caactttctt gtacaaagtg gttggtaagc
     4441 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt
     4501 ggcgcgttaa gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     4561 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     4621 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     4681 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     4741 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     4801 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     4861 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     4921 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     4981 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     5041 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     5101 cccgcgtcga ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     5161 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt
     5221 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     5281 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     5341 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     5401 atctctagca gtacgtatag tagttcatgt catcttatta ttcagtattt ataacttgca
     5461 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa
     5521 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     5581 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa
     5641 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     5701 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     5761 agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta
     5821 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     5881 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     5941 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg
     6001 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     6061 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     6121 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     6181 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     6241 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     6301 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     6361 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     6421 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc
     6481 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     6541 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     6601 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     6661 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     6721 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     6781 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     6841 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     6901 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     6961 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     7021 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     7081 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     7141 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     7201 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     7261 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     7321 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     7381 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     7441 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     7501 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     7561 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     7621 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     7681 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     7741 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     7801 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     7861 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     7921 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     7981 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     8041 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     8101 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     8161 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     8221 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     8281 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     8341 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     8401 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     8461 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     8521 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     8581 acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa
     8641 caaaagctgg agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca
     8701 tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat
     8761 tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat
     8821 ggattggacg aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc
     8881 gatacataaa cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     8941 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     9001 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     9061 aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct
     9121 ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact
     9181 ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag
     9241 cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg
     9301 gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt
     9361 cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct
     9421 acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac
     9481 cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat
     9541 agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac
     9601 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
     9661 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
     9721 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
     9781 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
     9841 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
     9901 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
     9961 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
    10021 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg
    10081 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
    10141 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
    10201 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
    10261 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
    10321 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccttgcgcc
    10381 ttttccaagg cagccctggg tttgcgcagg gacgcggctg ctctgggcgt ggttccggga
    10441 aacgcagcgg cgccgaccct gggtctcgca cattcttcac gtccgttcgc agcgtcaccc
    10501 ggatcttcgc cgctaccctt gtgggccccc cggcgacgct tcctgctccg cccctaagtc
    10561 gggaaggttc cttgcggttc gcggcgtgcc ggacgtgaca aacggaagcc gcacgtctca
    10621 ctagtaccct cgcagacgga cagcgccagg gagcaatggc agcgcgccga ccgcgatggg
    10681 ctgtggccaa tagcggctgc tcagcagggc gcgccgagag cagcggccgg gaaggggcgg
    10741 tgcgggaggc ggggtgtggg gcggtagtgt gggccctgtt cctgcccgcg cggtgttccg
    10801 cattctgcaa gcctccggag cgcacgtcgg cagtcggctc cctcgttgac cgaatcaccg
    10861 acctctctcc ccagggggta ccaccggagc ttaccatgac cgagtacaag cccacggtgc
    10921 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg
    10981 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc
    11041 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
    11101 acggcgccgc cgtggcggtc tggac
//
