LOCUS       sequence-190721-        1078 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  to epress PCP-GFP fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Qin P, Parlak M, Kuscu C, Bandaria J, Mir M, Szlachta K, Singh R, 
            Darzacq X, Yildiz A, Adli M
  TITLE     Live cell imaging of low- and non-repetitive chromosome loci using 
            CRISPR-Cas9.
  JOURNAL   Nat Commun. 2017 Mar 14;8:14725. doi: 10.1038/ncomms14725.
  PUBMED    28290446
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-03-14"; volume: "8:14725. doi"; pages: " 
            10.1038/ncomms14725"
COMMENT     Sequence Label: PCP-GFP
FEATURES             Location/Qualifiers
     source          1..1078
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(373..399)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             complement(373..399)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(403..423)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      426..435
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(438..836)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
ORIGIN
        1 tttcgtgggc gacagatctt cgacctgcga ggtcgcgacg agggacttgg tcaaatcgta
       61 caacgatttg cgcgaggcct cggtgctatt cgcaacgatt gtcacgtcgt gcgaccatac
      121 ctgagtgtag cgcactttcg gaagtccgga atcaacgacg tccgcctgat ccagttttag
      181 gttgacgcga tacgcggtct tggctccgtt ttgacggagc gaagccgtga ggcgcagccg
      241 acccaccaga ggcccgacct tctcttcgaa gatctgacgg tctgcggtgg actggatctc
      301 agtcagagtg cgagtagcct cgccgaccga aagaacgatg gttttggagg ctagcggatc
      361 cgggccttcc atggcgtagt cgggcacgtc gtaggggtag ccaacttttc ttttcttttt
      421 tgggcccatg gcggccgcca agtgacgatc acagcgatcc acaaacaaga accgcgaccc
      481 aaatcccggc tgcgacggaa ctagctgtgc cacacccggc gcgtccttat ataatcatcg
      541 gcgttcaccg ccccacggag atccctccgc agaatcgccg agaagggact acttttcctc
      601 gcctgttccg ctctctggaa agaaaaccag tgccctagag tcacccaagt cccgtcctaa
      661 aatgtccttc tgctgatact ggggttctaa ggccgagtct tatgagcagc gggccgctgt
      721 cctgagcgtc cgggcggaag gatcaggacg ctcgctgcgc ccttcgtctg acgtggcagc
      781 gctcgccgtg aggagggggg cgcccgcggg aggcgccaaa acccggcgcg gaggccacta
      841 gtccaaactg gatctctgct gtccctgtaa taaacccgaa attttgattt ttgtaatttg
      901 ttttgtattc tttagtttgt atgtctgttg ctattatgtc tactattctt tcccctgcac
      961 tgtaccccca atcccccttt ctttaaattg tgatgatact gccattgtga ttcggcgata
     1021 ccgtcagatc gtcactatcg atggatctgc tctgcctctt tcacttcttc ttcattct
//
