LOCUS       sequence-19079-e         949 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Choudhury A, Dominguez M, Puri V, Sharma DK, Narita K, Wheatley CL, 
            Marks DL, Pagano RE
  TITLE     Rab proteins mediate Golgi transport of caveola-internalized 
            glycosphingolipids and correct lipid trafficking in Niemann-Pick C 
            cells.
  JOURNAL   J Clin Invest. 2002 Jun . 109(12):1541-50.
  PUBMED    12070301
REFERENCE   2  (bases 1 to 949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2002 Jun . 109(12):1541-50."
COMMENT     Sequence Label: DsRed-rab9 DN
FEATURES             Location/Qualifiers
     source          1..949
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      48..57
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(54..56,57..59,60..731)
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /translation="MVRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH
                     HLFL"
     primer_bind     complement(237..257)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     455..474
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     649..672
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    735..791
                     /label=MCS
                     /note="multiple cloning site"
     CDS             795..821
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             822..848
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tagcagagct ggtttagtga ccgtcagatc cgctagcgct accggtcgcc accatggtgc
       61 gctcctccaa gaacgtcatc aaggagttca tgcgcttcaa ggtgcgcatg gagggcaccg
      121 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggccaca
      181 acaccgtgaa gctgaaggtg accaagggcg gccccctgcc cttcgcctgg gacatcctgt
      241 ccccccagtt ccagtacggc tccaaggtgt acgtgaagca ccccgccgac atccccgact
      301 acaagaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg
      361 gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggctgcttc atctacaagg
      421 tgaagttcat cggcgtgaac ttcccctccg acggccccgt aatgcagaag aagaccatgg
      481 gctgggaggc ctccaccgag cgcctgtacc cccgcgacgg cgtgctgaag ggcgagatcc
      541 acaaggccct gaagctgaag gacggcggcc actacctggt ggagttcaag tccatctaca
      601 tggccaagaa gcccgtgcag ctgcccggct actactacgt ggactccaag ctggacatca
      661 cctcccacaa cgaggactac accatcgtgg agcagtacga gcgcaccgag ggccgccacc
      721 acctgttcct gctcagatct cgagctcaag cttcgaattc tgcagtcgac ggtaccgcgg
      781 gcccgggatc catgtaccca tacgatgttc cggattacgc ttacccatac gatgttccgg
      841 attacgctgg taccgcagga aaatcatcac tttttaaagt aattctcctt ggagatggtg
      901 gagttgggaa gaattcactt atgaacagat atgtaactaa taagtttga
//
