LOCUS       sequence-190843-       12869 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  caCas9 integrating vector into the Neut5L locus with gene drive for 
            targeting Leu2 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12869)
  AUTHORS   Shapiro RS, Chavez A, Porter CBM, Hamblin M, Kaas CS, DiCarlo JE, 
            Zeng G, Xu X, Revtovich AV, Kirienko NV, Wang Y, Church GM, Collins 
            JJ
  TITLE     A CRISPR-Cas9-based gene drive platform for genetic interaction 
            analysis in Candida albicans.
  JOURNAL   Nat Microbiol. 2018 Jan;3(1):73-82. doi: 10.1038/s41564-017-0043-0. 
            Epub 2017 Oct 23.
  PUBMED    29062088
REFERENCE   2  (bases 1 to 12869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41564-017-0043-0"; journalName: "Nat Microbiol"; date: 
            "2018-01"; volume: "3"; issue: "1"; pages: "73-82"
COMMENT     Sequence Label: pJK-caCas9-NatMX-Neut5L-Leu2 drive
FEATURES             Location/Qualifiers
     source          1..12869
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..4104
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             1..4104
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4117..4137
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     4142..4158
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     4143..4159
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(5095..5292)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     promoter        complement(5893..6236)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     primer_bind     complement(7199..7218)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7200..7218)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7225..7242)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7225..7241)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7234..7256)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(7470..7489)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     terminator      complement(7742..7761)
                     /label=SUP4 terminator
                     /note="transcription terminator for the S. cerevisiae SUP4 
                     tRNA gene"
     misc_RNA        complement(7766..7840)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     9487..9508
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9671..9775
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9776..9844,9845..10636)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9994..10013)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10807..11395
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11296..11315
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11549..11566
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11683..11704
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11719..11724,11725..11742,11743..11749)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11757..11773
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11762..11784
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11781..11797
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11781..11797
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        11818..11836
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 atggataaaa aatactccat aggtttggac attggaacca attcagttgg atgggctgta
       61 atcactgatg aatataaggt tccttctaaa aagttcaaag ttttaggaaa tactgataga
      121 cactccatta aaaagaattt aattggtgcc ttgttgtttg acagtggtga aacagctgaa
      181 gcaactcgtt tgaaaagaac agctagaaga agatatacca gaagaaagaa cagaatttgt
      241 tacttgcaag aaatcttttc taatgaaatg gcaaaggttg atgattcttt ttttcataga
      301 ttggaagaaa gtttcttagt tgaagaagat aagaaacatg aaagacatcc aatctttggt
      361 aatattgtag atgaagtggc ttaccacgaa aaatatccta caatttatca tttgagaaaa
      421 aaattagttg actccactga taaagctgac ttaagactta tatatttggc attggctcac
      481 atgatcaagt tcagaggtca cttcttaatt gaaggtgatt tgaatccaga taattccgat
      541 gtcgacaagt tgtttattca attggttcaa acttacaacc aacttttcga ggaaaatcca
      601 atcaatgcct caggtgtgga tgctaaagct attttatctg ctagattgtc aaaaagtaga
      661 cgtttggaaa atcttattgc ccaattgcca ggtgaaaaaa aaaatggatt gtttggaaac
      721 ttaattgcat tatctttggg tttgacccca aatttcaaat caaactttga tttggccgag
      781 gatgctaaac ttcaattgtc taaggataca tacgatgacg atttagataa tcttcttgcc
      841 caaattggtg atcaatatgc agatttattc ttggcagcta agaacttgag tgatgcaatt
      901 ttgttaagtg acattttgag agtcaacaca gaaattacaa aagctccttt atccgcctct
      961 atgattaaaa gatatgatga acaccatcaa gatttaacct tgttgaaagc tcttgtacgt
     1021 caacaattac cagaaaagta taaagaaata ttctttgatc aatctaaaaa tggttacgct
     1081 ggttatattg atggtggtgc tagtcaagaa gaattttata agtttatcaa gccaattctt
     1141 gaaaaaatgg atggtactga ggaattgttg gttaagttga atcgagagga tttacttcgt
     1201 aagcaaagaa catttgataa cggttctatc cctcatcaaa tccacttagg tgagttacac
     1261 gctatcttga gacgtcagga agatttttac ccttttttga aagacaatcg tgaaaaaatt
     1321 gaaaaaattt tgactttcag aattccttat tacgtcgggc cattggctag aggtaattct
     1381 agatttgcat ggatgactcg taaatctgaa gagactatca ccccatggaa ttttgaggaa
     1441 gttgttgata agggagcctc cgctcaatca ttcatcgaga gaatgaccaa tttcgacaaa
     1501 aacttgccaa atgagaaggt gttacctaaa cattctttgt tatatgaata tttcactgtt
     1561 tataatgaat taacaaaagt caaatacgtc actgaaggta tgagaaaacc agcattctta
     1621 agtggtgaac aaaagaaagc tatcgttgac ttgttattca aaactaatcg taaagtcact
     1681 gtcaaacaac ttaaagagga ctattttaag aaaatagagt gtttcgattc cgttgaaatc
     1741 agtggggttg aggatagatt taatgctagt ttaggaactt atcacgattt gttgaaaatt
     1801 atcaaagaca aagatttttt ggataatgaa gaaaacgaag atatcttgga ggacattgta
     1861 ttgactttga ctttgtttga ggatagagaa atgatcgaag aacgtttaaa aacttacgct
     1921 cacttattcg acgataaagt catgaaacaa ttaaaaagaa gaagatatac cggttgggga
     1981 agattatcta gaaagttgat taatggtatt agagataagc aaagtggtaa aaccatctta
     2041 gatttcctta aaagtgatgg ttttgcaaac agaaacttta tgcagttgat tcatgatgat
     2101 agtttgacct ttaaggaaga tattcaaaaa gctcaggttt ctggacaggg tgattcattg
     2161 catgaacata ttgccaactt agcagggtct ccagctatca aaaagggaat tttacaaact
     2221 gttaaagttg ttgatgagtt ggtgaaagtt atgggacgtc ataagcctga aaatatagtt
     2281 atagaaatgg ctagagaaaa tcaaacaact cagaaaggac aaaagaattc tcgtgaaaga
     2341 atgaaaagaa tagaagaagg aattaaagag ttgggttctc aaatcttaaa agaacaccca
     2401 gtcgaaaaca cccagttaca gaacgagaag ctttacttgt actatttgca aaacgggcgt
     2461 gacatgtatg tagatcaaga gttggatatc aatagacttt cagattacga tgtcgatcac
     2521 atagttcctc agtcattttt aaaagatgat tccattgaca acaaagtttt aacaagatct
     2581 gataagaata gaggtaaatc agacaatgtg ccatcagaag aagtggttaa gaaaatgaag
     2641 aactattgga gacaattgtt aaatgcaaag ttgattactc agagaaaatt tgacaactta
     2701 actaaagctg aaagaggagg attatccgaa ttagataaag ctggattcat aaaacgtcag
     2761 ttggttgaaa caagacaaat tactaaacat gtagcccaaa ttttagattc ccgaatgaat
     2821 actaaatatg atgaaaatga caaattaatc agagaggtca aagtgatcac attaaagtca
     2881 aagttggtgt cagatttccg aaaggatttt caattctaca aggtgagaga gatcaacaat
     2941 tatcatcatg ctcacgacgc atatttgaat gcagtggtag gaactgcctt aataaagaag
     3001 tatcctaagt tagaatccga attcgtgtat ggtgattata aagtctacga tgtcagaaag
     3061 atgatagcaa aatcagaaca agaaattggg aaagccaccg caaagtattt cttttactcc
     3121 aacatcatga atttctttaa gaccgaaatc accttggcta acggggaaat cagaaaacgt
     3181 cctttgattg aaacaaatgg agaaaccggt gaaattgtgt gggataaggg gagagatttt
     3241 gctactgtta gaaaagtttt atcaatgcct caagttaata ttgtaaagaa gactgaagtt
     3301 caaactggtg gtttctctaa ggaatccatt cttccaaaac gaaactcaga caagttgatc
     3361 gcccgaaaaa aagattggga tcctaagaaa tacggaggtt ttgattctcc tactgtcgca
     3421 tattctgtct tggtcgtggc taaagttgaa aagggtaaat ccaaaaaatt gaaatctgtg
     3481 aaagaattgt taggtattac catcatggag cgttcatcct ttgagaaaaa cccaattgac
     3541 tttctcgaag cgaagggcta taaagaagtg aagaaagatt tgattattaa gttgccaaag
     3601 tattctctct ttgaacttga aaatgggaga aagagaatgt tggcttcagc tggagaattg
     3661 caaaaaggaa acgagttagc tttgccttca aagtacgtta atttcttata cttagcatct
     3721 cattatgaaa agttgaaggg gtcccctgaa gacaatgagc aaaagcaatt attcgtagaa
     3781 caacataaac actatttgga tgagataatt gaacaaatct ccgagttttc taaaagagtt
     3841 attttggctg atgcaaattt ggataaagtt ttatctgctt ataataagca tcgtgacaaa
     3901 ccaattcgag aacaagctga aaatattatc cacttgttca cattgactaa cttgggagct
     3961 ccagccgcgt ttaagtactt tgacaccacc attgaccgta agagatatac ttcaacaaaa
     4021 gaagtgttag acgccacatt gattcatcag tccataactg gtttatatga aactagaatc
     4081 gatttgtcac aattgggggg tgatagccga gcggatccta agaaaaaacg aaaagtctga
     4141 ctcgaggtcg acggtatcga taagcttgat atcgaattcc tgcagcccgg gggatccgag
     4201 tgaaattctg gaaatctgga aatctggttt tgtattcttg ttattcttct ttttgttatt
     4261 acatatataa cttgttactt ttttaaaaaa atctttgttt attttataaa tatataaaac
     4321 taaatttaag aaaaagagaa aaatgtttta tttgagagat tgaaatttta cttgaattta
     4381 gcttagcttt tataaagtat tattatgtaa aaaaacaaaa caaatataca ttaaaaagtt
     4441 aagactataa aatagccacc caaggcattt ctatatcttg ttgttgttgt tttcatcttc
     4501 tgtatcagag gaacttattt tattattttc gtcacgggta ttttctcttg tttgatgatt
     4561 catcccattc attccatcat aaaattctag aaggaccacc tttgattgta aatagtaata
     4621 attaccaccc ttatttaatt atttatttaa cttatttatt tatttattat acatatatac
     4681 aaatctaata aagtgaaaat ctcccccttc acatttataa ttggccagtt tttttcaaat
     4741 aagcattcca accagcatct ctataccttt taccttcaat atctggatct cttcctttac
     4801 caaacaatcc tctaccaaca atgataatat cagttccagt gctaacaact tcatcaacag
     4861 ttctatattg ttgtcctaat ccatcacctt tatcatctaa tccaactcca ggtgtcataa
     4921 taagccaatc aaatccttct tcttgtccac ccatatcacg ttgggcaata aatccaataa
     4981 caaattcctt atcggattta gcaatttcaa cagttttttg agaatattct ccatacgaat
     5041 tcgagctcgt ttaaacgtcg acactggatg gcggcgttag tatcgaatcg acagcagtat
     5101 agcgaccagc attcacatac gattgacgca tgatattact ttctgcgcac ttaacttcgc
     5161 atctgggcag atgatgtcga ggcgaaaaaa aatataaatc acgctaacat ttgattaaaa
     5221 tagaacaact acaatataaa aaaactatac aaatgacaag ttcttgaaaa caagaatctt
     5281 tttattgtca gtactgactc gagttattat ggacatggca tagacatata caaagcttgt
     5341 tcaccatcgg aagcagtacc atcgtataaa gcagtatcca aaccacacaa agtgaaaccc
     5401 attcttctat aagcatgaat agctggagca ttaacattgg taacttccaa ccacaaatga
     5461 ccagcacctc tttctctggc gaattcagta gccaaaccca tcaaagctct accaacacca
     5521 tgacctctat gttctggagc aacttcaata tcttcaacag tcaatcttct gttccaacca
     5581 gaataagaaa caacaacgaa accagccaaa tcaccatcat caccataagc aacgaaagtt
     5641 ctagaatctg gatcaccatc ttcaccagca tcggattcat catcggattc atcatctggg
     5701 aaaaccttag tcaatggtgg atcaactgga acttctctca aagtgaaacc atcaccagta
     5761 gcagtaactc taaaaacagt atcggtagtg aaagaaccat ccaaagcttc aatagcttca
     5821 gcatcacctg gaacagaagt tctgtatcta taagcagtat catccaaagt agtagacata
     5881 attgtaggat ccggttgttt atgttcggat gtgatgtgag aactgtatcc tagcaagatt
     5941 ttaaaaggaa gtatatgaaa gaagaacctc agtggcaaat cctaaccttt tatatttctc
     6001 tacaggggcg cggcgtgggg acaattcaac gcgtctgtga ggggagcgtt tccctgctcg
     6061 caggtctgca gcgaggagcc gtaatttttg cttcgcgccg tgcggccatc aaaatgtatg
     6121 gatgcaaatg attatacatg gggatgtatg ggctaaatgt acgggcgaca gtcacatcat
     6181 gcccctgagc tgcgcacgtc aagactgtca aggagggtat tctgggcctc catgtcgctg
     6241 gccgggtgac ccggcgggga cgaggcaagc ttgatatgct aatgatccca ctgatgataa
     6301 ttcagctaac atcaataacc ctcttggctc ttggttggtg gtggtttctt tagctccctg
     6361 ttttaatcct tcaactactc cattcccagt gacaccatga gcattagtaa tatctgccca
     6421 actactaatt ttataaactc caccaatata ttgtttcttc acggtattac caatatcagc
     6481 aaattttcta tcttcaaaaa tcataaattg atgtttacgt gaaagttcta ataatggttc
     6541 aatagtggat tcataggaaa aatcattgat tatatcaata tgagtcttga ttaagcatac
     6601 ataaggaccc aatttatcaa ttaattcaag gaattcctta gtggtatcaa catcaattga
     6661 tgcacataaa ttggttttct tcagttccat taatcgaaat aatcgttgtg ctactggtga
     6721 ggcatgagtt tctgctctct cactataggt cttagtgttg actgtcatat cttttgtttg
     6781 ctatttctat ttctatttct ttcttcaact gtaaaagggg cgatatataa accagtgtgt
     6841 atggggttgt tgctcaggaa ttgatcaatg tttaaaagaa tggttgaaat catcattgaa
     6901 gaacaaaaaa aaagattttt tctttcatta tgttatctgt ataatatgca ggaaataaga
     6961 ttgcaaaaaa atagccgttt ttttggatac tatcaaacaa gaggaatgac taatacagaa
     7021 ttagtgttac gaatcaatgg cactacagca actttcaaca aaataatcta cgttcaaaat
     7081 ataatatata ctattaaata aaatcaaata ataaaataat tttaaagaaa gtagtaccag
     7141 ctcttttttt tgtttccgtt tataccatcc aaatcaattc ctattagtct ccaattcgcc
     7201 ctatagtgag tcgtattaca attcactggc cgtcgtttta caacgtcgtg actgggaaaa
     7261 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     7321 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     7381 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     7441 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     7501 cacgttcgcc tgaggatgaa gagtttttct ctccgaatga agagccaaat catatacgag
     7561 agccaagtca atccgaatca agcgaggcca ccattacatc taccaggcaa aaaaattttg
     7621 caaataagca acctttattg agtgaagatt cggtaactca gttgtgtgca tatttagaga
     7681 aatctgtaga ctttggagta tttgaaattt aaatgctaac tactgtatat actgtgcggc
     7741 cagacataaa aaacaaaaaa agcaccaccg actcggtgcc actttttcaa gttgataacg
     7801 gactagcctt attttaactt gctatttcta gctctaaaac ttactgtctt gcctggtgac
     7861 caaattaaaa atagtttacg caagtctcga acttccacca caatttaatt gtaagaacgc
     7921 tgcgaaaaca tattctccaa tcctattggg ccgaagtttc agctatgcca aaggcatcaa
     7981 attttttttt ataaaatttt ccacttttcc ataccagtga aattatcacg tgatggagtg
     8041 agtatctata atatttgatt gtttctttga tgaggcaata ctcgagtata gttattgacc
     8101 aatgactttt agcgtttttt ggatattggt tttaaaagaa aggaataaga aaaggggagt
     8161 atttctggag tgaaaaaaaa aaaattcatt tgttgaaaaa agggcacccc agaaggagcg
     8221 ccggaatagc aaaatcacaa cctcatgctc gctccggtac aggcaaaaaa aaaattattc
     8281 aaagtaatct ctttctatac atgataaata cacggctaaa caagtggtat tcaagcacaa
     8341 ttcagttttc tggatctcta ataattgcaa ttgcaattgc ttcacatact ctgtttataa
     8401 ttcagtttct ttctcttgat gttactgata tattaagtta ataaatacca ataacttttt
     8461 ttcaaaaatc ttctattttc ctttcaattg attatttaaa cacgaatcgt taataagctg
     8521 tgattgcttt ttctctttct ttttcttttc tttgttcaag tttgaaaaaa aaaaaaaaag
     8581 gtttctcttg ttcgatgtag aattcagtgg gttatagttg taatgatata tggaataaaa
     8641 agaaagaaag agaagagaaa gagaagaaga gaagaattaa gtaaacgtgt attagaattt
     8701 aaaaattaaa attcatgata atctttaata ataattaact aagaatcaca aaattgaaaa
     8761 tttgaaagaa aaaagatcac tccaaggtcg tgtcaagtga ctagaaattc ttaattaaaa
     8821 tagttataac aaaaaaagaa atttgtatat aacaattaag taaaagaaaa aaaagcaatt
     8881 cgctttgtat aataataccc agaaaaatat attcatatat tcataaatca aacatgaaat
     8941 gaagaatgct gaatcacttg ataggattta gttccattat ggtactaaga agtaatatta
     9001 cttctatgaa aaggttgaat ccgagatcaa aattcatctt ttcatatact ctggagaaaa
     9061 aaaaaaaagt aattgtagta agaatgacaa gtatcagttg agaaaaaaag taacaaagag
     9121 aaagctcgga ggaggctccc caaaggtttt atcaccaatg gtggtaccac taacccaaga
     9181 acagaaaaag cattgatatt ttgaaaaata caatactcat cttttaaccc aaaccttttc
     9241 ttgtccttta aaacttttat ctcatgtctg tccaaagtca tattgtctta ctactttctc
     9301 tccttcaacg tccttccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     9361 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     9421 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     9481 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     9541 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     9601 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg
     9661 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     9721 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     9781 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     9841 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     9901 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     9961 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
    10021 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
    10081 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
    10141 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
    10201 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
    10261 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
    10321 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
    10381 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
    10441 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
    10501 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
    10561 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
    10621 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
    10681 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
    10741 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
    10801 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
    10861 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
    10921 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
    10981 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
    11041 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
    11101 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
    11161 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
    11221 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
    11281 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
    11341 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
    11401 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
    11461 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
    11521 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
    11581 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
    11641 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
    11701 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
    11761 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agctcgaaat
    11821 taaccctcac taaagggaac aaaagctggt accagagcta ttaagatcac cagcctcgtt
    11881 tataataaac ttagtctaat atttcttttg gaccaaaata gaaactgttc tcaggtggca
    11941 aaaaacagct taaagtctgt gttaaaaaaa gatctcggct atgccaatca aaaaggagtg
    12001 acattattat gtaaaagaag aaatgttgta attccgcgaa acaacaattt taatgcttac
    12061 tgtgccgcca ccaatatcct atggccaagg ggcctttaaa catgccacgg aaatatacag
    12121 tcaaagtaaa cctactatgt ctttagagcc ttcaggatca attgttttta aatgaatgac
    12181 tgcaaaacta gaaaagtagc ctcttacata agcaagtcat ttgtacaccg agagtttagt
    12241 aaccagtgaa gcaagtatat ctggcaaacc tgaaacttat ctgtgaatta taaagctcat
    12301 aagtaaaaag tgtagaaaag aagataaggc agaccaaata caaatttgca acagtacaac
    12361 gaatatttca gcgtcaaaac tagagaataa taaagaaaac gatcttttca aaaagaaaaa
    12421 accttttagt tttcctttgt tgttgttgtg ggtgtgtgct atttatatta tatagtttac
    12481 tcataatacc ataaaatatt cggtttgatt aggttatttt aataagctaa tttgtttcta
    12541 atcgtgtaat ttatgctgtg tatattaagt agtgtgtgca ctgcccaaaa atgtttgttg
    12601 tttatagtcg gttaaagaga aaaaagaaaa aaagatccat acacacacgt taattagttg
    12661 ttcaacgtaa tacactcata ttttgttctt atttgctttc ggtcgctgtt ctcaccaaga
    12721 tttattgcca acgaaacaat ttttttttat atattttcag atttttcttt ttttcctttc
    12781 ctttcctttt ctaattttca ctcctggttt tctttctttc ttagaaacat tatctcgata
    12841 ttaatattaa aaaaatataa tcattcaaa
//
