LOCUS       sequence-190859-        5905 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase validation vector with IFIT2 enhancer inserted downstream
            of the reporter gene. The candidate's enhancer activity is reflected
            by luciferase activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5905)
  AUTHORS   Muerdter F, Boryn LM, Woodfin AR, Neumayr C, Rath M, Zabidi MA, 
            Pagani M, Haberle V, Kazmar T, Catarino RR, Schernhuber K, Arnold 
            CD, Stark A
  TITLE     Resolving systematic errors in widely used enhancer activity assays 
            in human cells.
  JOURNAL   Nat Methods. 2017 Dec 11. pii: nmeth.4534. doi: 10.1038/nmeth.4534.
  PUBMED    29256496
REFERENCE   2  (bases 1 to 5905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2017 Dec 11. pii: nmeth.4534. doi: 10.1038/nmeth.4534."
COMMENT     Sequence Label: STARR-seq luciferase INF enhancer vector_ORI_I...
FEATURES             Location/Qualifiers
     source          1..5905
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    1678..1799
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1715..1734)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1769..1788
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(1843..3495)
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     regulatory      3492..3501
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     rep_origin      3600..4188
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4089..4108
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4378..5169,5170..5238))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5396..5413
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggagggaaca aaccaccatt ggtaagacag atttacaacc aacagaggta gaggcatagt
       61 aaggctataa acaaacacta aaatggagct ggccctcttt gggaacatag cttctcagga
      121 tttgcattgg acaagatgac tcaacagcac taccgaatac gaagggaaaa tatttacctc
      181 atggttgcag tgcaattctc agctgttcgg cagggctcgg ttcaggcagc tgcactcttc
      241 agaaatcttc ctcttctgcc accaataaat gctgaagaga cctatatata caagtggcct
      301 ctggttcctt tttgtttgtt tcccttcagc tgacgttaca aaagggaaag tgaaactaga
      361 aagtgaaatt ggcaggactc tttttctttt tttcttttta ttatacttta gaggactctt
      421 tttcctccgg agctgagttg tgatcagaga aagaaggcag cagagaggga tgtttcatcg
      481 gcctggtgct tcacttaaag atgggtctgg ggacattgct cttgtgcact gtccctctct
      541 tatttcatat tcgaaaagat taagttgggg acttgtaata caattattta acaaaagggt
      601 tcttttcagc agatactgtt aaagatatac acagaatagc aaaccccact tcctgctaag
      661 gtgaatttta cttttaatgt agtccttcat ctcagatttg cacaagggct ggtaaactta
      721 gatattttca ccccaaaatg ctcttgagta agagccaagc ccagctgcgt agtgagttag
      781 aagaacttcc tctttgtgga atgtactgat ggcatcaagg gagcacaact cctaggagga
      841 cacatttaca aagctccctg ccttgtgatt accatattaa cccatctttg ctctgcctta
      901 taccaatatg atccacaggc cactgtaagg agcagttatt tagcctcgac agggcagggg
      961 attttagtct gaatcattgc tgaatctcca gtccctgcaa aacaacctgc acattgcatg
     1021 gtatggcaag cattccaaaa gtagttgttg actaaatgaa tacatgatta gaagcatggg
     1081 cagtgatgtc tgtctatctg gatttgaaat cagcttccac ctttatgcta tgaaactctg
     1141 gggaaaatta ttgaatgtct ctgcttcggt tttgtaaatt gggaataata caactactgg
     1201 tttattgtgg tgttgggagg atgaaatgag agaatacgtg aaatgtcctt agaataatgc
     1261 ctggcagtaa taagtaaatg ctagtttcta ttataattat taactaaaat cgttaaccaa
     1321 agtgatcaga ggggaaactc tttttttcag gctggagtgc agtggaaaga tcctggctta
     1381 ctgcaacctc cacctccctg gttcaagtga ttcgcctcag cctctcgagt agctgggatt
     1441 acaggcacgc accaccacgc ccggctaatt tttgtatttt tagtagagat ggggttccac
     1501 catgtagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc
     1561 atcggtcgac ggatccttat cgattttacc acatttgtag aggttttact tgctttaaaa
     1621 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     1681 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     1741 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     1801 catgtctgct cgaagcggcc ggccgccccg actctagaat tattacacgg cgatcttgcc
     1861 gcccttcttg gccttaatga gaatctcgcg gatcttgcgg gcgtccaact tgccggtcag
     1921 tcctttaggc acctcgtcca cgaacacaac accaccgcgc agcttcttgg cggttgtaac
     1981 ctggctggcc acatagtcca cgatctcctt ctcggtcatg gttttaccgt gttccagcac
     2041 gacgactgcg gcgggcagct cgccggcatc gtcgtcgggc aggccggcga ccccggcgtc
     2101 gaagatgttg gggtgttgca gcaggatgct ctccagttcg gctggggcta cctggtagcc
     2161 cttgtatttg atcaggctct tcagccggtc cacgatgaag aagtgctcgt cctcgtccca
     2221 gtaggcgatg tcgccgctgt gcagccagcc gtccttgtcg atgagagcgt ttgtagcctc
     2281 ggggttgtta acgtagccgc tcatgatcat ggggccacgg acgcacagct cgccgcgctg
     2341 gttcacaccc agtgtcttac cggtgtccaa gtccaccacc ttagcctcga agaagggcac
     2401 caccttgcct actgcgccag gcttgtcgtc cccttcgggg gtgatcagaa tggcgctggt
     2461 tgtttctgtc aggccgtagc cctggcggat gcctggtagg tggaagcgtt tggccacggc
     2521 ctcacctacc tccttgctga gcggcgcccc gccgctggcg atctcgtgca agttgcttag
     2581 gtcgtacttg tcgatgagag tgctcttagc gaagaagcta aatagtgtgg gcaccagcag
     2641 ggcagattga atcttatagt cttgcaagct gcgcaagaat agctcctcct cgaagcggta
     2701 catgagcacg acccgaaagc cgcagatcaa gtagcccagc gtggtgaaca tgccgaagcc
     2761 gtggtgaaat ggcaccacgc tgaggatagc ggtgtcgggg atgatctggt tgccgaagat
     2821 ggggtcgcgg gcatgactga atcggacaca agcggtgcgg tgcggtaggg ctacgccctt
     2881 gggcaatccg gtactgccac tactgttcat gatcagggcg atggttttgt cccggtcgaa
     2941 gctctcgggc acgaagtcgt actcgttgaa gccgggtggc aaatgggaag tcacgaaggt
     3001 gtacatgctt tggaagccct ggtagtcggt cttgctatcc atgatgatga tcttttgtat
     3061 gatcggtagc ttcttttgca cgttgaggat cttttgcagc cctttcttgc tcacgaatac
     3121 gacggtgggc tggctgatgc ccatgctgtt cagcagctcg cgctcgttgt agatgtcgtt
     3181 agctggggcc acagccacac cgatgaacag ggcacccaac acgggcatga agaactgcaa
     3241 gctattctcg ctgcacacca cgatccgatg gtttgtattc agcccatagc gcttcatagc
     3301 ttctgccagc cgaacgctca tctcgaagta ctcggcgtag gtaatgtcca cctcgatatg
     3361 tgcgtcggta aaggcgatgg tgccgggcac cagggcgtag cgcttcatgg ctttgtgcag
     3421 ctgctcgccg gcggtcccgt cttcgagtgg gtagaatggc gctgggccct tcttaatgtt
     3481 tttggcatct tccatggtgg ctttaccaac agtaccggat tgccaagctt caaaatccct
     3541 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3601 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3661 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3721 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3781 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3841 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3901 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3961 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     4021 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     4081 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     4141 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     4201 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtc atgagattat
     4261 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     4321 gtatatatga gtaaacttgg tctgacagcg gccgcaaatg ctaaaccact gcagtggtta
     4381 ccagtgcttg atcagtgagg caccgatctc agcgatctgc ctatttcgtt cgtccatagt
     4441 ggcctgactc cccgtcgtgt agatcactac gattcgtgag ggcttaccat caggccccag
     4501 cgcagcaatg atgccgcgag agccgcgttc accggccccc gatttgtcag caatgaacca
     4561 gccagcaggg agggccgagc gaagaagtgg tcctgctact ttgtccgcct ccatccagtc
     4621 tatgagctgc tgtcgtgatg ctagagtaag aagttcgcca gtgagtagtt tccgaagagt
     4681 tgtggccatt gctactggca tcgtggtatc acgctcgtcg ttcggtatgg cttcgttcaa
     4741 ctctggttcc cagcggtcaa gccgggtcac atgatcaccc atattatgaa gaaatgcagt
     4801 cagctcctta gggcctccga tcgttgtcag aagtaagttg gccgcggtgt tgtcgctcat
     4861 ggtaatggca gcactacaca attctcttac cgtcatgcca tccgtaagat gcttttccgt
     4921 gaccggcgag tactcaacca agtcgttttg tgagtagtgt atacggcgac caagctgctc
     4981 ttgcccggcg tctatacggg acaacaccgc gccacatagc agtactttga aagtgctcat
     5041 catcgggaat cgttcttcgg ggcggaaaga ctcaaggatc ttgccgctat tgagatccag
     5101 ttcgatatag cccactcttg cacccagttg atcttcagca tcttttactt tcaccagcgt
     5161 ttcggggtgt gcaaaaacag gcaagcaaaa tgccgcaaag aagggaatga gtgcgacacg
     5221 aaaatgttgg atgctcatac tcgtcctttt tcaatattat tgaagcattt atcagggtta
     5281 ctagtacgtc tctcaagaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     5341 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     5401 gtcagtgagc gaggaagcgg aagagcgctg ccggcacctg tcctacgagt tgcatgataa
     5461 agaagacagt cataagtgcg gcgacgattt tctccaagca ctccatctct aattcatact
     5521 caggaggcat tttggggtga aaatatttaa gtttaccagc ccttgtgcaa atctgagatg
     5581 aagaactaca ttaaaagtaa aattcacctt tgcaggaagt ggggtttgct attctgtgta
     5641 tatctttaac agtatctgct gaaaagaacc cttttgttaa ataattgtat tacaagtccc
     5701 caacttaatc ctttcaaata tgaaataaga cagggtcagt gcacaagagc aatgccccca
     5761 gacccatctt taagtgaagc accaggccga tgaaacatca tccctctctg ctgccttctt
     5821 tctctgattg caactcaact ccccaggcgt gcagttctgg agatgaattt aggaagcttg
     5881 ccagtgtgaa gcagaaaatt gttca
//
