LOCUS       sequence-19093-e         704 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf8
FEATURES             Location/Qualifiers
     source          1..704
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             330..371
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             381..398
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(421..438)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    427..652
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 tggtaccgag ctcggatcca gtacccttca ccatggttgc atcgtgcttt aacatcaatg
       61 gtacaaattt tatcctcgct ttgtgtatca tattcgtctc tagtataaaa ttctatattc
      121 agattatcat gcgatgtgta tacgctaacg gtatcaataa acggagcaca ccatttagtc
      181 ataacagtaa tccaaaattt tttaaagtat atcttaacga aagaagttgt gtcattgtct
      241 acggtgtatg gtactagaag ggtcaagaca attctgcaga tatccagcac agtggcggcc
      301 gctcgagtct agagggcccg cggttcgaag gtaagcctat ccctaaccct ctcctcggtc
      361 tcgattctac gcgtaccggt catcatcacc atcaccattg agtttaaacc cgctgatcag
      421 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
      481 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
      541 attgtctgag taggtgtcat tctattcggg gggtgggggg tggggcagga cagcaagggg
      601 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
      661 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgcc
//
