LOCUS       sequence-190952-        9132 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  All-in-one lentivector with tet-inducible DUX4 (wildetype nucleotide
            sequence).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9132)
  AUTHORS   Jagannathan S, Shadle SC, Resnick R, Snider L, Tawil RN, van der 
            Maarel SM, Bradley RK, Tapscott SJ
  TITLE     Model systems of DUX4 expression recapitulate the transcriptional 
            profile of FSHD cells.
  JOURNAL   Hum Mol Genet. 2016 Oct 15;25(20):4419-4431. doi: 
            10.1093/hmg/ddw271.
  PUBMED    28171552
REFERENCE   2  (bases 1 to 9132)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Hum Mol Genet"; date: "2016-10-15- 15"; volume: "25"; issue: "20"; 
            pages: "4419-4431"
COMMENT     Sequence Label: pCW57.1-DUX4-WT
FEATURES             Location/Qualifiers
     source          1..9132
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     142..159
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    276..297
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(312..317,318..335,336..342)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    350..366
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     355..377
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     374..390
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     374..390
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     409..429
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        411..429
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        457..683
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             684..864
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    911..1036
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1529..1762
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1947..1991
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2204..2321
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2375..2689
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2383..2401
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2419..2437
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2454..2472
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2490..2508
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2526..2544
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2561..2579
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2597..2615
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2662..2686
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2742..2751
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(4049..4065)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4050..4066)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    complement(4068..4192)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             4210..4227
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        4237..4747
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4666..4684
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             4757..5353
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4757..4776)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5253..5273
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             5354..5407
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(5408..6025,6026..6031,6032..6067,6068..6070,6071..6106,
                     6107..6109,6110..6145,6146..6154)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(6194..6214)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6612..6623)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6828..7061
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7133..7254
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7170..7189)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7224..7243
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7294..7429
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7429..7500
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7501..7569,7570..8361)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7719..7738)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8532..9120
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9021..9040
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
       61 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
      121 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
      181 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
      241 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
      301 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
      361 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct
      421 cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt
      481 agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc
      541 gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga
      601 cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta
      661 agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc
      721 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
      781 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
      841 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
      901 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
      961 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
     1021 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     1081 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     1141 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     1201 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     1261 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     1321 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     1381 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     1441 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     1501 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     1561 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     1621 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     1681 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     1741 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     1801 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     1861 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     1921 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     1981 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     2041 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     2101 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     2161 gacaattctc gacctcgaga caaatggcag tattcatcca caattttaaa agaaaagggg
     2221 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     2281 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     2341 gcagagatcc actttggccg cgaatcgata tgtcgagttt actccctatc agtgatagag
     2401 aacgtatgtc gagtttactc cctatcagtg atagagaacg atgtcgagtt tactccctat
     2461 cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag
     2521 tttactccct atcagtgata gagaacgtat gtcgagttta tccctatcag tgatagagaa
     2581 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgaggta ggcgtgtacg
     2641 gtgggaggcc tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagaattgg
     2701 ctagaactag tggatccccc gggctgcagg aattcgccct tgccaccatg gccctcccga
     2761 caccctcgga cagcaccctc cccgcggaag cccggggacg aggacggcga cggagactcg
     2821 tttggacccc gagccaaagc gaggccctgc gagcctgctt tgagcggaac ccgtacccgg
     2881 gcatcgccac cagagaacgg ctggcccagg ccatcggcat tccggagccc agggtccaga
     2941 tttggtttca gaatgagagg tcacgccagc tgaggcagca ccggcgggaa tctcggccct
     3001 ggcccgggag acgcggcccg ccagaaggcc ggcgaaagcg gaccgccgtc accggatccc
     3061 agaccgccct gctcctccga gcctttgaga aggatcgctt tccaggcatc gccgcccggg
     3121 aggagctggc cagagagacg ggcctcccgg agtccaggat tcagatctgg tttcagaatc
     3181 gaagggccag gcacccggga cagggtggca gggcgcccgc gcaggcaggc ggcctgtgca
     3241 gcgcggcccc cggcgggggt caccctgctc cctcgtgggt cgccttcgcc cacaccggcg
     3301 cgtggggaac ggggcttccc gcaccccacg tgccctgcgc gcctggggct ctcccacagg
     3361 gggctttcgt gagccaggca gcgagggccg cccccgcgct gcagcccagc caggccgcgc
     3421 cggcagaggg gatctcccaa cctgccccgg cgcgcgggga tttcgcctac gccgccccgg
     3481 ctcctccgga cggggcgctc tcccaccctc aggctcctcg gtggcctccg cacccgggca
     3541 aaagccggga ggaccgggac ccgcagcgcg acggcctgcc gggcccctgc gcggtggcac
     3601 agcctgggcc cgctcaagcg gggccgcagg gccaaggggt gcttgcgcca cccacgtccc
     3661 aggggagtcc gtggtggggc tggggccggg gtccccaggt cgccggggcg gcgtgggaac
     3721 cccaagccgg ggcagctcca cctccccagc ccgcgccccc ggacgcctcc gcctccgcgc
     3781 ggcaggggca gatgcaaggc atcccggcgc cctcccaggc gctccaggag ccggcgccct
     3841 ggtctgcact cccctgcggc ctgctgctgg atgagctcct ggcgagcccg gagtttctgc
     3901 agcaggcgca acctctccta gaaacggagg ccccggggga gctggaggcc tcggaagagg
     3961 ccgcctcgct ggaagcaccc ctcagcgagg aagaataccg ggctctgctg gaggagcttt
     4021 agaagggcga attcgatatc aagcttatcg ataccgtcga cctcgaccat agtgactgga
     4081 tatgttgtgt tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat
     4141 tgatatttat atcattttac gtttctcgtt cagctttctt gtacaaagtg gtttagtaat
     4201 gaaccggtcc accaccacca ccaccactaa ggatccgggg ttggggttgc gccttttcca
     4261 aggcagccct gggtttgcgc agggacgcgg ctgctctggg cgtggttccg ggaaacgcag
     4321 cggcgccgac cctgggtctc gcacattctt cacgtccgtt cgcagcgtca cccggatctt
     4381 cgccgctacc cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa gtcgggaagg
     4441 ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc tcactagtac
     4501 cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat gggctgtggc
     4561 caatagcggc tgctcagcag ggcgcgccga gagcagcggc cgggaagggg cggtgcggga
     4621 ggcggggtgt ggggcggtag tgtgggccct gttcctgccc gcgcggtgtt ccgcattctg
     4681 caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt gaccgaatca ccgacctctc
     4741 tccccagcaa ttcaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     4801 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     4861 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
     4921 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt
     4981 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     5041 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     5101 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
     5161 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     5221 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
     5281 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
     5341 caagcccggt gccgaaggta gaggttctct cctcacttgt ggtgatgttg aagaaaaccc
     5401 tggtccaatg tctagactgg acaagagcaa agtcataaac ggagctctgg aattactcaa
     5461 tggtgtcggt atcgaaggcc tgacgacaag gaaactcgct caaaagctgg gagttgagca
     5521 gcctaccctg tactggcacg tgaagaacaa gcgggccctg ctcgatgccc tgccaatcga
     5581 gatgctggac aggcatcata cccacttctg ccccctggaa ggcgagtcat ggcaagactt
     5641 tctgcggaac aacgccaagt cataccgctg tgctctcctc tcacatcgcg acggggctaa
     5701 agtgcatctc ggcacccgcc caacagagaa acagtacgaa accctggaaa atcagctcgc
     5761 gttcctgtgt cagcaaggct tctccctgga gaacgcactg tacgctctgt ccgccgtggg
     5821 ccactttaca ctgggctgcg tattggagga acaggagcat caagtagcaa aagaggaaag
     5881 agagacacct accaccgatt ctatgccccc acttctgaga caagcaattg agctgttcga
     5941 ccggcaggga gccgaacctg ccttcctttt cggcctggaa ctaatcatat gtggcctgga
     6001 gaaacagcta aagtgcgaaa gcggcgggcc gaccgacgcc cttgacgatt ttgacttaga
     6061 catgctccca gccgatgccc ttgacgactt tgaccttgat atgctgcctg ctgacgctct
     6121 tgacgatttt gaccttgaca tgctccccgg gtaactaagt aaggatcgat ccaagatatc
     6181 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     6241 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     6301 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     6361 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     6421 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     6481 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga
     6541 cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     6601 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     6661 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     6721 cctccccgcc tgtttcgcct cggcgtccgg actagaggta cctttaagac caatgactta
     6781 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     6841 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     6901 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     6961 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     7021 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     7081 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
     7141 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     7201 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     7261 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     7321 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     7381 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct ttcaaatatg
     7441 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     7501 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     7561 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     7621 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     7681 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     7741 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     7801 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     7861 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     7921 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     7981 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     8041 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     8101 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     8161 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     8221 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     8281 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     8341 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     8401 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     8461 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     8521 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     8581 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     8641 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     8701 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     8761 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     8821 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     8881 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     8941 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     9001 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     9061 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     9121 aacgccagca ac
//
