LOCUS       sequence-190986-        2215 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BB1 with insert: terminator ScCYC1tt.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Prielhofer R, Barrero JJ, Steuer S, Gassler T, Zahrl R, Baumann K, 
            Sauer M, Mattanovich D, Gasser B, Marx H
  TITLE     GoldenPiCS: a Golden Gate-derived modular cloning system for applied
            synthetic biology in the yeast Pichia pastoris.
  JOURNAL   BMC Syst Biol. 2017 Dec 8;11(1):123. doi: 10.1186/s12918-017-0492-3.
  PUBMED    29221460
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Syst 
            Biol."; date: "2017-12-8"; volume: "11(1):123. doi"; pages: " 
            10.1186/s12918-017-0492-3"
COMMENT     Sequence Label: BB1_34_ScCYC1tt
FEATURES             Location/Qualifiers
     source          1..2215
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     40..57
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     41..57
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      212..459
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(228..246)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(551..567)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(551..567)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      615..646
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        647..749
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             750..1565
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCLKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(823..842)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1589..1616
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1628..2215)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1708..1727)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgattttcta ccgaagaaag gcccacccgt gtaaaacgac ggccagttta
       61 tctagtcagc ttgattctag ctgatcgtgg accggaaggt gagccagtga gttgattgca
      121 gtccagttac gctggagtct gaggctcgtc ctgaatgata tgcggcctcg aattcgaaga
      181 cgcgcttcag gccccttttc ctttgtcgat atcatgtaat tagttatgtc acgcttacat
      241 tcacgccctc cccccacatc cgctctaacc gaaaaggaag gagttagaca acctgaagtc
      301 taggtcccta tttatttttt tatagttatg ttagtattaa gaacgttatt tatatttcaa
      361 atttttcttt tttttctgta cagacgcgtg tacgcatgta acattatact gaaaaccttg
      421 cttgagaagg ttttgggacg ctcgaaggct ttaatttgca agctcgctgc gtcttcgaat
      481 tcgcgcgtga tcttacggca ttatacgtat gatcggtcca cgatcagcta gattatctag
      541 tcagcttgat gtcatagctg tttcctgagg ctcaatactg accatttaaa tcatacctga
      601 cctccatagc agaaagtcaa aagcctccga ccggaggctt ttgacttgat cggcacgtaa
      661 gaggttccaa ctttcaccat aatgaaataa gatcactacc gggcgtattt tttgagttat
      721 cgagattttc aggagctaag gaagctaaaa tgagccatat tcaacgggaa acgtcttgct
      781 tgaagccgcg attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg
      841 ataatgtcgg gcaatcaggt gcgacaatct atcgattgta tgggaagccc gatgcgccag
      901 agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca
      961 ggctaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc
     1021 ctgatgatgc atggttactc accactgcga tcccagggaa aacagcattc caggtattag
     1081 aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt
     1141 tgcattcgat tcctgtttgt aattgtcctt ttaacggcga tcgcgtattt cgtctcgctc
     1201 aggcgcaatc acgaatgaat aacggtttgg ttggtgcgag tgattttgat gacgagcgta
     1261 atggctggcc tgttgaacaa gtctggaaag aaatgcataa actcttgcca ttctcaccgg
     1321 attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat
     1381 taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca
     1441 tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat
     1501 atggtattga taatcctgat atgaataaat tgcagtttca cttgatgctc gatgagtttt
     1561 tctaatgagg acctaaatgt aatcacctgg ctcaccttcg ggtgggcctt tctgcgttgc
     1621 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga tgctcaagtc
     1681 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     1741 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     1801 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     1861 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     1921 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     1981 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     2041 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     2101 cagttacctc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     2161 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaa
//
