LOCUS       sequence-19105-e         792 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf21
FEATURES             Location/Qualifiers
     source          1..792
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             645..686
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             696..713
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(736..753)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 taccgagctc ggatccagta cccttcaccg aattccagct gaccaccatg gggaactgct
       61 gcaacaattc agactcccac caaattaatg aataaagaaa atgcagaaat gattttggaa
      121 aaaattgttg atcatatagt tatgtatatt agtgacgaat caagtgattc agaaaataat
      181 cctgaatata ttgattttcg taacagatac gaagactata gatctctcat tataaaaagt
      241 gatcacgagt ttgtaaagct atgtaaaaat catgcagaga aaagttctcc agaaacgcaa
      301 caaatgatta tcaaacacat atacgaacaa tatcttattc cagtatctga agtactatta
      361 aaacctataa tgtccatggg tgacataatt acatataacg gatgtaaaga caatgaatgg
      421 atgctagaac aactctctac cctaaacttt aacaatctcc gcacatggaa ctcatgtagc
      481 ataggcaatg taacgcgtct gttttataca ttttttagtt atctgatgaa agataaacta
      541 aatatataag caattcccgg ggatccgaat aaagggtcaa gacaattctg cagatatcca
      601 gcacagtggc ggccgctcga gtctagaggg cccgccggtt cgaaggtaag cctatcccta
      661 accctctcct cggtctcgat tctacgcgta ccggtcatca tcaccatcac cattgagttt
      721 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
      781 cccccgtgcc tt
//
