LOCUS       sequence-191054-        4315 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-terminal AID*-9myc degron cassette with HIS3MX marker for S. 
            cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4315)
  AUTHORS   Morawska M, Ulrich HD
  TITLE     An expanded tool kit for the auxin-inducible degron system in 
            budding yeast.
  JOURNAL   Yeast. 2013 Sep;30(9):341-51. doi: 10.1002/yea.2967. Epub 2013 Jul 
            23.
  PUBMED    23836714
REFERENCE   2  (bases 1 to 4315)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.2967"; 
            journalName: "Yeast"; date: "2013-09"; volume: "30"; issue: "9"; 
            pages: "341-51"
COMMENT     Sequence Label: pHis-AID*-9myc
FEATURES             Location/Qualifiers
     source          1..4315
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             79..210
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Morawska and Ulrich, 
                     2013)"
                     /label=AID*
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="PKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEAAAFVK"
     CDS             232..261
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             268..297
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             304..333
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             352..381
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             388..417
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             424..453
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             472..501
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             508..537
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             544..573
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     gene            648..1848
                     /label=HIS3MX6
                     /note="yeast selectable marker encoding the S. pombe his5 
                     gene, which corresponds to S. cerevisiae HIS3"
     promoter        648..991
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             992..1645
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe his5"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=S. pombe his5
                     /note="yeast auxotrophic marker; corresponds to S. 
                     cerevisiae HIS3"
                     /translation="MGRRAFVERNTNETKISVAIALDKAPLPEESNFIDELITSKHANQ
                     KGEQVIQVDTGIGFLDHMYHALAKHAGWSLRLYSRGDLIIDDHHTAEDTAIALGIAFKQ
                     AMGNFAGVKRFGHAYCPLDEALSRSVVDLSGRPYAVIDLGLKREKVGELSCEMIPHLLY
                     SFSVAAGITLHVTCLYGSNDHHRAESAFKSLAVAMRAATSLTGSSEVPSTKGVL"
     terminator      1651..1848
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(1952..1971)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1953..1971)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2058..2075)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2229..2817)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2309..2328)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2988..3779,3780..3848))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3611..3630
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3849..3953)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4021..4039
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4077..4099)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4199..4218
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        join(4299..4315,1..2)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     join(4299..4315,1)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggtgcagg cgccggggct
       61 ggtgctgggg caggtacccc taaagatcca gccaaacctc cggccaaggc acaagttgtg
      121 ggatggccac cggtgagatc ataccggaag aacgtgatgg tttcctgcca aaaatcaagc
      181 ggtggcccgg aggcggcggc gttcgtgaag gtatcacccg gggctagtgg tgaacaaaag
      241 ttgatttctg aagaagattt gaacggtgaa caaaagctaa tctccgagga agacttgaac
      301 ggtgaacaaa aattaatctc agaagaagac ttgaacggat cctctagagg tgaacaaaag
      361 ttgatttctg aagaagattt gaacggtgaa caaaagctaa tctccgagga agacttgaac
      421 ggtgaacaaa aattaatctc agaagaagac ttgaacggat cctctagagg tgaacaaaag
      481 ttgatttctg aagaagattt gaacggtgaa caaaagctaa tctccgagga agacttgaac
      541 ggtgaacaaa aattaatctc agaagaagac ttgaacggat ccactagcta aaaaggcgcg
      601 ccagatctgt ttagcttgcc tcgtccccgc cgggtcaccc ggccagcgac atggaggccc
      661 agaataccct ccttgacagt cttgacgtgc gcagctcagg ggcatgatgt gactgtcgcc
      721 cgtacattta gcccatacat ccccatgtat aatcatttgc atccatacat tttgatggcc
      781 gcacggcgcg aagcaaaaat tacggctcct cgctgcagac ctgcgagcag ggaaacgctc
      841 ccctcacaga cgcgttgaat tgtccccacg ccgcgcccct gtagagaaat ataaaaggtt
      901 aggatttgcc actgaggttc ttctttcata tacttccttt taaaatcttg ctaggataca
      961 gttctcacat cacatccgaa cataaacaac catgggtagg agggcttttg tagaaagaaa
     1021 tacgaacgaa acgaaaatca gcgttgccat cgctttggac aaagctccct tacctgaaga
     1081 gtcgaatttt attgatgaac ttataacttc caagcatgca aaccaaaagg gagaacaagt
     1141 aatccaagta gacacgggaa ttggattcct ggatcacatg tatcatgcac tggctaaaca
     1201 tgcaggctgg agcttacgac tttactcaag aggtgattta atcatcgatg atcatcacac
     1261 tgcagaagat actgctattg cacttggtat tgcattcaag caggctatgg gtaactttgc
     1321 cggcgttaaa agatttggac atgcttattg tccacttgac gaagcccttt ctagaagcgt
     1381 agttgacttg tcgggacggc cctatgctgt tatcgatttg ggattaaagc gtgaaaaggt
     1441 tggggaattg tcctgtgaaa tgatccctca cttactatat tccttttcgg tagcagctgg
     1501 aattactttg catgttacct gcttatatgg tagtaatgac catcatcgtg ctgaaagcgc
     1561 ttttaaatct ctggctgttg ccatgcgcgc ggctactagt cttactggaa gttctgaagt
     1621 cccaagcacg aagggagtgt tgtaaagagt actgacaata aaaagattct tgttttcaag
     1681 aacttgtcat ttgtatagtt tttttatatt gtagttgttc tattttaatc aaatgttagc
     1741 gtgatttata ttttttttcg cctcgacatc atctgcccag atgcgaagtt aagtgcgcag
     1801 aaagtaatat catgcgtcaa tcgtatgtga atgctggtcg ctatactgct gtcgattcga
     1861 tactaacgcc gccatccagt ttaaacgagc tcgaattcat cgatgatatc agatccacta
     1921 gtggcctatg cggccgcgga tctgccggtc tccctatagt gagtcgtatt aatttcgata
     1981 agccaggtta acctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     2041 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     2101 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     2161 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     2221 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     2281 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     2341 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     2401 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     2461 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     2521 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     2581 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     2641 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     2701 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     2761 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     2821 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     2881 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     2941 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     3001 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     3061 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     3121 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     3181 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     3241 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     3301 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     3361 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     3421 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     3481 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     3541 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     3601 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     3661 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     3721 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     3781 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     3841 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     3901 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     3961 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     4021 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
     4081 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     4141 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
     4201 gcatcagagc agattgtact gagagtgcac catatggaca tattgtcgtt agaacgcggc
     4261 tacaattaat acataacctt atgtatcata cacatacgat ttaggtgaca ctata
//
