LOCUS       sequence-19111-e         764 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf28
FEATURES             Location/Qualifiers
     source          1..764
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             544..585
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             595..612
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(635..652)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    641..751
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 tggtaccgag ctcggatcca gtacccttca ccgaattcca gctgaccacc atggtaaact
       61 tttgcatatc ggacgcagaa attggtataa atgttgtaat tgtattattt cccgtcaatg
      121 gactagtcac agctccatca gttttatatc ctttagagta tttctcactc gtgtctaaca
      181 ttctagagca ttccatgatc tgtttatcgt tgatattggc cggaaagata gattttttat
      241 tttttattat attactattg gcaattgtag atataacttc tggtaaatat ttttctacct
      301 tttcaatctc ttctattttc aagccggcta tatattctgc tatattgttg ctagtatcaa
      361 taccttttct ggctaagaag tcatatgtgg tattcactat atcagtttta actggtagtt
      421 ccattagcct ttccacttct gcagaataag caattcccgg ggatccgaat aaagggtcaa
      481 gacaattctg cagatatcca gcacagtggc ggccgctcga gtctagaggg cccgcggttc
      541 gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgcgtac cggtcatcat
      601 caccatcacc attgagttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
      661 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
      721 gtcctttcct aataaatgag gaaattgcat cgcattgtct gagt
//
