LOCUS       sequence-191153-        5558 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express CMYA5 (aa 4039 – 4069) with a N-terminal GST tag in 
            bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5558)
  AUTHORS   Hsiung A, Naya FJ, Chen X, Shiang R
  TITLE     A schizophrenia associated CMYA5 allele displays differential 
            binding with desmin.
  JOURNAL   J Psychiatr Res. 2019 Apr;111:8-15. doi: 
            10.1016/j.jpsychires.2019.01.007. Epub 2019 Jan 10.
  PUBMED    30658136
REFERENCE   2  (bases 1 to 5558)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Psychiatr
            Res."; date: "2019-04"; volume: "111:8-15. doi"; pages: " 
            10.1016/j.jpsychires.2019.01.007. Epub 2019 Jan 10"
COMMENT     Sequence Label: CMYA5/pDEST15
FEATURES             Location/Qualifiers
     source          1..5558
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      480..1068
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     969..988
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1222..1239
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    1254..1393
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(1319..1338)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1479..1501
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(1495..1686)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     3148..3167
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     3251..3270
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3251..3269
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             3301..3323
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             3331..3984
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     protein_bind    4018..4042
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(4219..4235)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4220..4236)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    complement(4246..4270)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(4334..4352)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      4348..4395
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(4761..4766,4767..4783,4784..4789))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(4816..4834)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4902..5006
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5007..5075,5076..5558,1..309)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5225..5244)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
       61 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
      121 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
      181 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
      241 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
      301 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
      361 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
      421 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
      481 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
      541 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
      601 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
      661 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
      721 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
      781 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
      841 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
      901 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
      961 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     1021 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     1081 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     1141 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     1201 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     1261 cggtattttc tccttacgca tctgtgcggt atttcacacc gcaatggtgc actctcagta
     1321 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg
     1381 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     1441 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     1501 gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc
     1561 gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag
     1621 aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt
     1681 ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa
     1741 acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg
     1801 ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg
     1861 tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc
     1921 tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta
     1981 cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca
     2041 gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc
     2101 ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga
     2161 cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat
     2221 gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat
     2281 tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc
     2341 cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca
     2401 atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc
     2461 ggtccaatga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga
     2521 tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg
     2581 gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag
     2641 acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt
     2701 ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca
     2761 agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag
     2821 agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg
     2881 acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc
     2941 atcggtcgat cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag
     3001 gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa
     3061 cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc
     3121 gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc
     3181 acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat
     3241 cccgcgaaat taatacgact cactataggg agaccacaac ggtttccctc tagaaataat
     3301 tttgtttaac tttaagaagg agatatacat atgtccccta tactaggtta ttggaaaatt
     3361 aagggccttg tgcaacccac tcgacttctt ttggaatatc ttgaagaaaa atatgaagag
     3421 catttgtatg agcgcgatga aggtgataaa tggcgaaaca aaaagtttga attgggtttg
     3481 gagtttccca atcttcctta ttatattgat ggtgatgtta aattaacaca gtctatggcc
     3541 atcatacgtt atatagctga caagcacaac atgttgggtg gttgtccaaa agagcgtgca
     3601 gagatttcaa tgcttgaagg agcggttttg gatattagat acggtgtttc gagaattgca
     3661 tatagtaaag actttgaaac tctcaaagtt gattttctta gcaagctacc tgaaatgctg
     3721 aaaatgttcg aagatcgttt atgtcataaa acatatttaa atggtgatca tgtaacccat
     3781 cctgacttca tgttgtatga cgctcttgat gttgttttat acatggaccc aatgtgcctg
     3841 gatgcgttcc caaaattagt ttgttttaaa aaacgtattg aagctatccc acaaattgat
     3901 aagtacttga aatccagcaa gtatatagca tggcctttgc agggctggca agccacgttt
     3961 ggtggtggcg accatcctcc aaaatcggat ctggttccgc gtccatggtc gaatcaaaca
     4021 agtttgtaca aaaaagcagg ctctttaaag gaaccgggct gcaggaattc gattgaattc
     4081 cttgtgcctt acagaatccc catggttaat gagggtgtcc accctgcctt tgccctggag
     4141 aaacctggaa aatgtacttt gcacctgggg atagagcccc cggattctgt aaggcacaag
     4201 gaattcaatc aagcttatcg ataccgtcga cctcgagata tctagaccca gctttcttgt
     4261 acaaagtggt ttgattcgac ccgggatccg gctgctaaca aagcccgaaa ggaagctgag
     4321 ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc
     4381 ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt
     4441 cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg
     4501 gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata
     4561 tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa
     4621 gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc
     4681 gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt
     4741 taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt
     4801 cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     4861 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     4921 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     4981 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     5041 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     5101 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     5161 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     5221 ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc
     5281 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     5341 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     5401 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     5461 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     5521 caaacgacga gcgtgacacc acgatgcctg cagcaatg
//
