LOCUS       sequence-19117-e         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf34
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             673..714
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             724..741
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cgagctcgga tccagtaccc ttcaccgaat tccagctgac caccatgaac ggcggacata
       61 ttcagttgat aatcggcccc atgttttcag gtaaaagtac agaattaatt agacgagtta
      121 gacgttatca aatagctcaa tataaatgcg tgactataaa atattctaac gataatagat
      181 acggaacggg actatggacg catgataaga ataattttga agcattggaa gcaactaaac
      241 tatgtgatgt cttggaatca attacagatt tctccgtgat aggtatcgat gaaggacagt
      301 tctttccaga cattgttgaa ttctgtgagc gtatggcaaa cgaaggaaaa atagttatag
      361 tagccgcact cgatgggaca tttcaacgta aaccgtttaa taatattttg aatcttattc
      421 cattatctga aatggtggta aaactaactg ctgtgtgtat gaaatgcttt aaggaggctt
      481 ccttttctaa acgattgggt gaggaaaccg agatagaaat aataggaggt aatgatatgt
      541 atcaatcggt gtgtagaaag tgttacatcg actcataagc aattcccggg gatccgaata
      601 aagggtcaag acaattctgc agatatccag cacagtggcg gccgctcgag tctagagggc
      661 ccgcggttcg aaggtaagcc tatccctaac cctctcctcg gtctcgattc tacgcgtacc
      721 ggtcatcatc accatcacca ttgagtttaa acccgctgat cagcctcgac tgtgccttct
//
