LOCUS       sequence-191282-         729 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor Vector containing BSX transcription factor, part of the Human 
            TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR221-BSX
FEATURES             Location/Qualifiers
     source          1..729
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(701..725)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atgaatctca acttcacctc tcctctacac ccggcgtctt ctcagaggcc cacatccttc
       61 ttcatcgagg acatcctgct gcacaagccc aagccgctga gagaggtggc cccagaccat
      121 ttcgccagct ctctggcctc tcgtgtgcct cttctagact atggctatcc cctcatgccc
      181 acacccaccc tcttggctcc tcacgcccat caccctctgc ataagggtga ccaccatcat
      241 ccttatttcc ttaccacctc ggggatgcca gtcccagcgc tattcccgca ccctcaacac
      301 gcggagctac ctgggaaaca ttgtcgtcgc cgcaaagcac gcacggtttt ctctgactcg
      361 cagctctcgg gcttggagaa gaggttcgag attcagcgct acctgtccac gccagaacga
      421 gtggagcttg ccacggccct cagcctatcc gagacgcagg tcaaaacgtg gttccagaac
      481 cggcggatga agcataaaaa gcaactgcgg aaaagccaag acgaacccaa agcaccagat
      541 gggccagaaa gcccagaagg aagtccccgc ggttcagaag ctgctaccgc tgctgaagct
      601 cggcttagcc tacctgccgg tcccttcgta ctgactgagc cagaggacga ggtggatatt
      661 ggagacgagg gtgagctggg ctcaggtccg cacgttctct gcccaacttt cttgtacaaa
      721 gttgtcccc
//
