LOCUS       sequence-191284-         588 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor Vector containing HES3 transcription factor, part of the Human
            TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 588)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR221-HES3
FEATURES             Location/Qualifiers
     source          1..588
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(560..584)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atggagaaaa agcgccgggc acgcatcaat gtgtcactgg agcagctcaa gtcgctgctg
       61 gagaaacact actcgcacca gatccggaag cgcaaattgg agaaggccga catcctggag
      121 ttgagcgtga agtacatgag aagccttcag aactccttgc aagggctctg gcctgtaccc
      181 agaggagccg agcaaccgtc gggcttccgt agctgcttgc ccggtgttag ccagctcctt
      241 aggcgtggag atgaggtagg cagcggactt cgttgtccac tagtgcccga gagcgccgcc
      301 ggtagcacca tggacagcgc agggttaggt caggaagcac cagcgttatt ccgaccttgc
      361 actcctgctg tctgggctcc tgctcctgca gccggaggcc cgagatctcc accacccttg
      421 ctactactcc ccgaaagtct tcctggctcg tctgctagcg ttcctccacc gcagccagct
      481 tctagtcgtt gcgctgagag tcccgggcta ggtttgcgtg tgtggcgacc atggggaagt
      541 cccggggatg acctgaactg cccaactttc ttgtacaaag ttgtcccc
//
