LOCUS       sequence-191289-        3477 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF705A transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 3477)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR221-ZNF705A
FEATURES             Location/Qualifiers
     source          1..3477
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(902..926)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(932..1031)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1048..1067)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1049..1067)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1072..1088)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1072..1088)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1190..1209)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1201..2010
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1268..1287)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2157..2745
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2646..2665
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2899..2916
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3075..3102)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3194..3280)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3329..3351
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3343..3360
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3344..3360
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3376..3475
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atgcattcac taaagaaagt gacttttgaa gatgtagcta ttgacttcac ccaggaagag
       61 tgggccatga tggacacatc caagagaaag ctgtacagag atgtgatgct ggaaaatatc
      121 agtcacctgg tgtccctcgg gtaccagata agcaaatcct atataatttt gcagctggag
      181 caaggaaaag agctgtggcg ggaaggaaga gaatttcttc aagaccagaa tccagacagg
      241 gaaagtgccc ttaagaaaaa acacatgata tccatgcatc ctatcaccag aaaagacgca
      301 tccaccagta tgacaatgga gaactctctc attctggagg atccttttga atgtaatgat
      361 tcgggagaag attgcactca cagttccaca ataactcagc gtttgttaac tcacagtgga
      421 aagaaaccct atgtcagcaa acagtgtgga aaatctctcc gtaacctttt ctccccgaag
      481 ccacataaac agatccatac taaaggtaaa tcgtatcagt gtaatctatg cgaaaaggcc
      541 tataccaatt gctttcgcct tagacggcac aagatgactc acactggaga gaggccatat
      601 gcatgtcatc tatgtggaaa agccttcact cagtgttctc accttagaag acatgagaaa
      661 actcacacgg gagagcgacc atataagtgt catcaatgcg ggaaagcctt tattcagtcc
      721 tttaaccttc gaagacacga gagaacccac cttggaaaaa agtgttatga atgcgataaa
      781 agtgggaaag cctttagcca aagctctggc tttagaggaa acaaaataat tcacaccgga
      841 gagaaaccac atgcttgtct tctatgtggg aaggccttca gtctgtcttc cgaccttaga
      901 tgcccaactt tcttgtacaa agttgtcccc tacccagctt tcttgtacaa agttggcatt
      961 ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa
     1021 atcattattt gccatccagc tgatatcccc tatagtgagt cgtattacat ggtcatagct
     1081 gtttcctggc agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa
     1141 aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt
     1201 atgagccata ttcaacggga aacgtcgagg ccgcgattaa attccaacat ggatgctgat
     1261 ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcgc
     1321 ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc
     1381 aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg
     1441 accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc
     1501 ggaaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat
     1561 gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac
     1621 agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat
     1681 gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg
     1741 cataaacttt tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat
     1801 aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc
     1861 gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca
     1921 ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag
     1981 tttcatttga tgctcgatga gtttttctaa tcagaattgg ttaattggtt gtaacactgg
     2041 cagagcatta cgctgacttg acgggacggc gcaagctcat gaccaaaatc ccttaacgtg
     2101 agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     2161 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     2221 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     2281 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     2341 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     2401 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     2461 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     2521 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     2581 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     2641 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     2701 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     2761 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     2821 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     2881 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     2941 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     3001 gactggaaag cgggcagtga gcgcaacgca attaatacgc gtaccgctag ccaggaagag
     3061 tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg
     3121 cagtttatgg cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat
     3181 ccgctcccgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac
     3241 gaaaggccca gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact
     3301 ctcgcgttaa cgctagcatg gatgttttcc cagtcacgac gttgtaaaac gacggccagt
     3361 cttaagctcg ggccccaaat aatgatttta ttttgactga tagtgacctg ttcgttgcaa
     3421 caaattgatg agcaatgctt ttttataatg ccaactttgt acaaaaaagc aggctta
//
