LOCUS       sequence-191469-        3449 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF705B transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 3449)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR221-ZNF705B
FEATURES             Location/Qualifiers
     source          1..3449
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(904..1003)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1020..1039)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1021..1039)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1044..1060)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1044..1060)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1162..1181)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1173..1982
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1240..1259)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2129..2717
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2618..2637
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2871..2888
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3047..3074)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3166..3252)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3301..3323
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3315..3332
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3316..3332
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3348..3447
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atgcacagcc tggagaaggt gaccttcgag gacgtggcca tcgacttcac ccaggaggag
       61 tgggacatga tggacaccag caagagaaag ctgtacagag acgtgatgct ggagaatatc
      121 tctcacctgg tgagcctggg ctaccagatc agcaagtcct acatcatcct gcagctggag
      181 cagggcaagg agctgtggtg ggagggcaga gtgtttctgc aggaccagaa ccccgacaga
      241 gagagcgccc tgaagaagaa gcacatgatc agcatgcacc ccatcatcag aaaggacacc
      301 tccaccagca tgaccatgga gaacagcctg atcctggagg accccttcga gtacaacgac
      361 agcggcgagg attgcaccca ctccagcacc atcacccagt gtctgctgac ccacagcggc
      421 aagaagccct gcgtgagcaa gcagtgcggg aagagcctga gaaacctgct gagccccaag
      481 cccagaaagc agatccacac caagggcaag agctaccagt gcaacctgtg tgagaaggcc
      541 tacaccaact gcttctacct gaggagacac aagatgacac acaccggaga gaggccttac
      601 gcctgccacc tgtgcggcaa ggcatttacc cagtgcagcc acctgagaag acacgagaag
      661 acccacaccg gcgagaggcc ctacaagtgc caccagtgtg gcaaagcctt catccagagc
      721 ttcaacctga gacggcacga gagaacccac ctgggccaga agtgctacga gtgcgacaag
      781 tctggcaagg ctttcagcca gagcagcggc ttcagaggca acaagatcat ccacatcggc
      841 gagaagcccc ccgcctgcct gctgtgcgga aaggccttca gcctgagcag cgacctgaga
      901 tatacccagc tttcttgtac aaagttggca ttataagaaa gcattgctta tcaatttgtt
      961 gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgccatcca gctgatatcc
     1021 cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc
     1081 aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt
     1141 ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtcga
     1201 ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata
     1261 atgtcgggca atcaggtgcg acaatctatc gcttgtatgg gaagcccgat gcgccagagt
     1321 tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac
     1381 taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg
     1441 atgatgcatg gttactcacc actgcgatcc ccggaaaaac agcattccag gtattagaag
     1501 aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc
     1561 attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg
     1621 cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg
     1681 gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt
     1741 cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa
     1801 taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc
     1861 tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg
     1921 gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct
     1981 aatcagaatt ggttaattgg ttgtaacact ggcagagcat tacgctgact tgacgggacg
     2041 gcgcaagctc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag
     2101 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     2161 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     2221 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     2281 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     2341 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     2401 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     2461 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     2521 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     2581 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     2641 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     2701 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     2761 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     2821 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     2881 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     2941 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     3001 caattaatac gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa ggccatccgt
     3061 caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca
     3121 ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg tcctactcag
     3181 gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtcttccg actgagcctt
     3241 tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt aacgctagca tggatgtttt
     3301 cccagtcacg acgttgtaaa acgacggcca gtcttaagct cgggccccaa ataatgattt
     3361 tattttgact gatagtgacc tgttcgttgc aacaaattga tgagcaatgc ttttttataa
     3421 tgccaacttt gtacaaaaaa gcaggctta
//
