LOCUS       sequence-191492-         573 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor Vector containing ASCL3 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 573)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR221-ASCL3
FEATURES             Location/Qualifiers
     source          1..573
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(545..569)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atgatggaca acagaggcaa ctctagtcta cctgacaaac ttcctatctt ccctgattct
       61 gcccgcttgc cacttaccag gtccttctat ctggagccca tggtcacttt ccacgtgcac
      121 ccagaggccc cggtgtcatc tccttactct gaggagctgc cacggctgcc ttttcccagc
      181 gactctctta tcctgggaaa ttacagtgaa ccctgcccct tctctttccc gatgccttat
      241 ccaaattaca gagggtgcga gtactcctac gggccagcct tcacccggaa aaggaatgag
      301 cgggaaaggc agcgggtgaa atgtgtcaat gaaggctacg cccagctccg acatcatctg
      361 ccagaggagt atttggagaa gcgactcagc aaagtggaaa ccctcagagc tgcgatcaag
      421 tacattaact acctgcagtc tcttctgtac cctgataaag ctgagacaaa gaataaccct
      481 ggaaaagttt cctccatgat agcaaccacc agccaccatg ctgaccctat gttcagaatt
      541 gtttgcccaa ctttcttgta caaagttgtc ccc
//
