LOCUS       sequence-19155-e         738 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf72
FEATURES             Location/Qualifiers
     source          1..738
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             625..666
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             676..693
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(716..733)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 tcaccgaatt ccagctgacc accatgaaat cgcttaatag acaaactgta agtaggttta
       61 agaagttgtc ggtgccggcc gctataatga tgatactctc aaccattatt agtggcatag
      121 gaacatttct gcattacaaa gaagaactga tgcctagtgc ttgcgccaat ggatggatac
      181 aatacgataa acattgttat ttagatacta acattaaaat gtctacagat aatgcggttt
      241 atcagtgtcg taaattacga gccagattgc ctagaccgga tactagacat ctgagagtat
      301 tgtttagtat tttttataaa gattattggg taagtttaaa aaagaccaat gataaatggt
      361 tagatattaa taatgataaa gatatagata ttagtaaatt aacaaatttt aaacaactaa
      421 acagtacgac ggatgctgaa gcgtgttata tatacaagtc tggaaaactg gttaaaacag
      481 tatgtaaaag tactcaatct gtactatgtg ttaaaaaatt ctacaagtga gcaattcccg
      541 gggatccgaa taaagggtca agacaattct gcagatatcc agcacagtgg cggccgctcg
      601 agtctagang gcccgcggtt cgaaggtaag cctatcccta accctctcct cggtctcgat
      661 tctacgcgta ccggtcatca tcaccatcac cattgagttt aaacccgctg atcagcctcg
      721 actgtgcctt ctagttgc
//
