LOCUS       sequence-191589-        4700 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF470 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4700)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 4700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR221-ZNF470
FEATURES             Location/Qualifiers
     source          1..4700
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(2155..2254)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2271..2290)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2272..2290)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2295..2311)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2295..2311)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2413..2432)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2424..3233
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2491..2510)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3380..3968
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3869..3888
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4122..4139
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4298..4325)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4417..4503)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4552..4574
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4566..4583
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4567..4583
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4599..4698
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atgaaatccc aagaagaagt agaagtggct ggaataaaat tgtgcaaggc aatgagcctt
       61 ggttctgtca ctttcactga tgtggcaatt gatttttccc aagatgaatg ggaatggcta
      121 aacctggccc agagatccct atataaaaaa gtgatgcttg aaaactacag aaaccttgtc
      181 agtgtcggcc tctgtatttc gaagccagat gtgatatccc tccttgaaca agagaaagac
      241 ccctgggtaa taaaaggtgg aatgaacagg ggtttgtgcc ctgatttaga gtgtgtctgg
      301 gtcacgaaat ccctcagcct aaaccaagat atatatgaag aaaaattgcc cccggcaata
      361 atcatggaga gattaaaatc gtatgacctt gagtgcagca cccttggaaa gaactggaag
      421 tgtgaagacc tttttgagag agaacttgta aaccaaaaga cccatttcag acaagagaca
      481 atcacccata tagacaccct aatagagaag agggaccatt cgaataagag tgggacggtt
      541 ttccacctaa acaccctatc gtacataaag cagattttcc cgatggaaga gaggattttt
      601 aatttccaca cagataagaa gagcctgaag acccacagcg ttgtgaagaa acacaaacaa
      661 gacaggggtg agaaaaaact cctaaagtgt aatgactgtg agaagatttt ttcaaagata
      721 tcgaccctca ccctccacca aagaatccac acgggagaga agccatatga atgtatagaa
      781 tgcggtaaag ctttcagcca atcggcccat ttagcacaac accaaaggat ccatactggg
      841 gaaaaacctt ttgaatgcac tgagtgcggg aaagcttttt cccaaaatgc acaccttgta
      901 caacaccaga gagtccacac tggagagaag ccataccaat gtaaacagtg taataaagct
      961 ttcagccagc ttgcccattt ggcccaacat caaagagtcc ataccggtga aaaaccatat
     1021 gagtgtattg aatgcggaaa ggctttcagt gactgttctt ctttagccca ccacagaagg
     1081 atacatactg gaaagaggcc atatgagtgc attgactgtg gaaaggcatt cagacaaaat
     1141 gccagtttga tcagacacag gagatattac catactggag aaaagccttt tgactgtata
     1201 gactgtggga aggcattcac tgaccatatt ggtttaatcc aacataagag aacccatacc
     1261 ggagaaaggc catataaatg taatgtctgc gggaaggctt tttcccatgg cagcagcctc
     1321 actgtccacc aaagaattca cactggtgaa aagccatatg aatgtaatat atgtgagaaa
     1381 gctttcagcc acagaggcag cctaacccta caccaaagag tacatacagg tgagaaacca
     1441 tatgaatgta aagagtgtgg gaaagcattc agacagagta cccaccttgc ccaccaccag
     1501 agaatccata caggagagaa gccgtatgaa tgtaaagaat gttcaaaaac tttttcacag
     1561 aatgcccacc tagcccaaca ccaaaaaata cataccgggg agaagccata tgagtgtaag
     1621 gagtgcggaa aagcattttc acaaatagcc catcttgtcc aacaccaaag ggtccatact
     1681 ggtgagaagc catatgaatg tattgagtgt ggaaaggcat tcagtgatgg atcatacctt
     1741 gtccagcacc aaagactcca ttctggaaaa aggccatatg aatgcctaga atgtggaaaa
     1801 gctttcagac aaagagcgag tttaatctgc caccaaaggt gccacaccgg agagaaaccg
     1861 tatgagtgta atgtgtgtgg taaggctttt tcccacagaa aatccctgac tttacaccag
     1921 aggatacaca caggagaaaa accatatgaa tgtaaagagt gttcaaaggc attttcccaa
     1981 gtggcccacc tgaccctcca taagaggatc cacacaggtg aaagaccata tgagtgtaaa
     2041 gagtgcggta aggcattcag acaatctgta cacctagcac accaccaaag aatacacacc
     2101 ggtgagagtt ctgtaatcct ttcgagtgcc ctcccatacc accaagtttt atatacccag
     2161 ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac
     2221 aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgatatc ccctatagtg
     2281 agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct
     2341 gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca
     2401 taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat
     2461 taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc
     2521 aatcaggtgc gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga
     2581 aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc
     2641 tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat
     2701 ggttactcac cactgcgatc cccggaaaaa cagcattcca ggtattagaa gaatatcctg
     2761 attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc
     2821 ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac
     2881 gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg
     2941 ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca
     3001 ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta
     3061 ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact
     3121 gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata
     3181 atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat
     3241 tggttaattg gttgtaacac tggcagagca ttacgctgac ttgacgggac ggcgcaagct
     3301 catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag
     3361 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     3421 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     3481 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     3541 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     3601 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     3661 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     3721 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     3781 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     3841 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     3901 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     3961 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     4021 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     4081 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     4141 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     4201 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata
     4261 cgcgtaccgc tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc
     4321 cttctgctta gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg
     4381 ccgttgcttc acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt
     4441 caccgacaaa caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat
     4501 ttgatgcctg gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac
     4561 gacgttgtaa aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac
     4621 tgatagtgac ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt
     4681 tgtacaaaaa agcaggctta
//
