LOCUS       sequence-191594-        3491 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor Vector containing ZNF516 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR221-ZNF516
FEATURES             Location/Qualifiers
     source          1..3491
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      2373..2382
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggacagaa acagagaagc agaaatggaa ttgagaagag gaccttcacc tacaagggct
       61 ggaagaggcc atgaagtaga tggtgataaa gctacgtgcc atacctgctg tatctgtgga
      121 aagagtttcc ctttccaatc aagtttaagc caacatatga ggaaacatac tggagaaaag
      181 ccatataaat gcccgtactg tgaccaccgg gcaagccaaa aaggaaacct aaaaatccat
      241 ataagaagcc acaggactgg cactttaatc caaggacatg agccagaggc tggtgaagcc
      301 cctttaggtg aaatgagggc cagtgaaggc ctagatgcgt gtgcgagccc cacaaagagt
      361 gcatcggcat gtaacagact tttaaatgga gcttcccagg ctgatggagc gagagtttta
      421 aacggtgcca gccaggcaga cagcgggaga gtccttttga gatcttcaaa aaaaggtgca
      481 gaagggagtg catgtgcccc aggtgaagca aaagcagctg tccaatgttc tttctgtaag
      541 agccaatttg aaagaaaaaa agaccttgaa ctccatgtcc accaggccca taaacctttt
      601 aagtgcagac tatgttcata tgcaactttg agagaagaaa gccttttatc ccatatagag
      661 agagatcata tcacggccca aggccctgga agtggagagg cctgtgtaga aaatggaaag
      721 ccagaattat cgcctggaga attcccctgt gaagtctgtg gccaagcttt cagccagacg
      781 tggttcctaa aggcacatat gaagaaacac agaggcagtt ttgaccatgg atgccatata
      841 tgtgggagga gatttaaaga gccatggttc ctgaagaacc atatgaaagc ccatggacca
      901 aagactggtt caaagaacag gccaaaatct gaattagacc caattgctac aataaataat
      961 gttgtccagg aagaagtaat cgtggctggc ctcagcctat atgaagtgtg tgcaaaatgt
     1021 ggaaaccttt ttacaaacct tgattcttta aatgcacata atgcaatcca cagaagagtt
     1081 gaagccagca ggaccagggc gcctgctgaa gaaggagcag aaggccccag tgacacaaaa
     1141 caatttttcc tccagtgcct aaacctcagg ccaagtgcag ctggtgattc gtgccctggt
     1201 acccaagctg gaaggagagt tgcagaacta gaccctgtaa attcatacca ggcatggcaa
     1261 ttggctacca gagggaaagt agcagagcct gcagaatacc taaaatatgg ggcgtgggat
     1321 gaagctttag ccggagatgt tgcttttgat aaagacagaa gagaatatgt ccttgtaagc
     1381 caagaaaaga ggaagagaga acaagatgcc cctgcggcac aaggaccccc cagaaaaaga
     1441 gcatctggcc ctggtgaccc tgccccagct ggacaccttg acccaagatc agcagcgagg
     1501 ccaaacagaa gggctgcggc aaccactggc caaggaaaat ccagtgaatg ttttgagtgt
     1561 ggaaaaatat tcaggacata ccaccaaatg gtcctacaca gcagagtcca caggagagcg
     1621 aggagagaaa gagattctga tggtgacagg gcagcgagag caagatgtgg aagcctgagt
     1681 gaaggagatt cagcgagcca accatcgagc cctggatcgg cgtgtgctgc agctgacagc
     1741 cctggttctg gcctagctga tgaggcagca gaagacagtg gagaagaagg tgcacctgag
     1801 cctgcgccag gtggccagcc caggagatgc tgtttcagtg aagaagtcac atctactgaa
     1861 ttaagcagtg gtgaccagag ccataaaatg ggagataatg ccagtgagag agacactggt
     1921 gaatcaaagg cagggatagc tgccagtgta tcgatccttg aaaattcatc gagagaaaca
     1981 tccaggagac aagaacaaca cagattttca atggacctga aaatgcctgc tttccacccg
     2041 aaacaagaag tccctgtgcc tggtgatggt gttgaattcc catcgagtac aggggcagaa
     2101 ggacagacag gccacccagc agaaaaatta tctgacctcc ataataaaga acacagcgga
     2161 ggaggaaaga gggctttggc cccagaccta atgccccttg acctcagtgc gaggagcacc
     2221 agagatgacc cttcgaataa agaaactgca tcttccctcc aggcagccct tgttgtccac
     2281 ccatgcccat actgttccca taagacatat tacccagaag ttttgtggat gcataaaaga
     2341 atctggcaca gagtttcatg taattctgtt gcgccgccat ggatccagcc aaatggatat
     2401 aaatcaatca gatcaaacct tgtatttttg agcagatcag ggagaactgg cccgccgccg
     2461 gccctcggtg gaaaagaatg ccaacccctc ctcctggcga gatttacgag aacccaagta
     2521 ccaggtggaa tgcctggatc aaaaagtgga tcatcgcccc ttggtgttgt gacaaaggcg
     2581 gcttcaatgc caaaaaataa agagagccac agtggtggcc cgtgtgccct gtgggcacct
     2641 gggcctgatg gatacagaca gacaaaaccg tgccatggcc aagaacccca tggagcagca
     2701 acccaaggac ctttggcaaa gcccagacaa gaggcttcat caaagcctgt cccggcgcct
     2761 ggcggtggtg gtttttcaag aagtgcaacg ccgacgccga cagtaattgc gagggcaggg
     2821 gcccagccga gtgcaaattc gaagcctgtt gaaaaatttg gtgtcccccc ggctggggct
     2881 ggttttgccc ctacaaataa acacagtgcg cctgacagtt taaaagcaaa attttcggcc
     2941 caaccccaag ggccaccgcc ggcaaaagga gaaggtggag cgccaccttt accacccaga
     3001 gaaccccctt caaaggctgc ccaagaattg aggactttag ccacatgtgc tgctggatcg
     3061 agaggtgatg cggccctcca agcccagcct ggtgttgctg gtgcaccccc tgttttacat
     3121 tcaataaaac aagagccggt ggcggaaggc catgagaaga gattagatat tttgaacatt
     3181 tttaagacgt atatcccgaa agattttgcc accctatacc aaggatgggg tgtaagtgga
     3241 cctggccttg aacacagagg gaccctgagg acccaggcca gaccaggaga atttgtatgt
     3301 atagaatgtg ggaaaagttt ccaccaacct ggccatttga gggcccatat gagagcacac
     3361 agtgttgttt ttgaatcaga tggaccgaga gggagtgaag tccacactac gagtgcagat
     3421 gcaccaaaac aaggaaggga ccattcaaac actggaactg tccagactgt acccctaaga
     3481 aaaggtacat a
//
