LOCUS       sequence-19170-e         676 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf87
FEATURES             Location/Qualifiers
     source          1..676
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             427..468
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             478..495
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(518..535)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    524..635
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ccgaattcca gctgaccacc atgataatat taaaagatgg atataaagaa tttgctgact
       61 gcatgtacta ttttttacat tactacattg gctacggcag atatacctac tccgccacca
      121 acgggtcatg tgacaaggga gaatatcttg ataagaggca taatcaatgt tgtaatcggt
      181 gtccacctgg agaatttgcc aaggttagat gtaatggtaa cgataacaca aaatgtgaac
      241 gctgcccacc tcatacatat accacaatcc caattattct aatggatgtc atcaatgtag
      301 aaaatgccca accggatcat ttgataaggt aagcaattcc cggggatccg aataaagggt
      361 caagacaatt ctgcagatat ccagcacagt ggcggccgct cgagtctaga gggcccgcgg
      421 ttcgaaggta agcctatccc taaccctctc ctcggtctcg attctacgcg taccggtcat
      481 catcaccatc accattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
      541 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
      601 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcttcta
      661 tctggggggg gggggg
//
