LOCUS       sequence-19171-e         593 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 593)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf88
FEATURES             Location/Qualifiers
     source          1..593
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             362..403
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             413..430
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(453..470)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    459..570
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 accgaattcc agctgaccac catggatata aagaatttgc tgactgcatg tactattttt
       61 tacattacta cattggctac ggcagatata cctactccgc caccaacggg tcatgtgaca
      121 agggagaata tcttgataag aggcataatc aatgttgtaa tcggtgtcca cctggagaat
      181 ttgccaaggt tagatgtaat ggtaacgata acacaaaatg tgaacgctgc ccacctcata
      241 catataccac aatcccaatt attctaagca attcccgggg atccgaataa agggtcaaga
      301 caattctgca gatatccagc acagtggcgg ccgctcgagt ctagagggcc cgcggttcga
      361 aggtaagcct atccctaacc ctctcctcgg tctcgattct acgcgtaccg gtcatcatca
      421 ccatcaccat tgagtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
      481 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
      541 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc att
//
