LOCUS       sequence-191746-        3602 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes the fluorescent protein eqFP486 for constitutive expression 
            in E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Molina RS, Tran TM, Campbell RE, Lambert GG, Salih A, Shaner NC, 
            Hughes TE, Drobizhev M
  TITLE     Blue-Shifted Green Fluorescent Protein Homologues Are Brighter than 
            Enhanced Green Fluorescent Protein under Two-Photon Excitation.
  JOURNAL   J Phys Chem Lett. 2017 Jun 15;8(12):2548-2554. doi: 
            10.1021/acs.jpclett.7b00960. Epub 2017 May 25.
  PUBMED    28530831
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jpclett.7b00960"; journalName: "J Phys Chem Lett"; 
            date: "2017-06-15- 15"; volume: "8"; issue: "12"; pages: "2548-2554"
COMMENT     Sequence Label: pRSM17
FEATURES             Location/Qualifiers
     source          1..3602
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     33..52
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     286..303
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     388..407
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        388..406
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             532..549
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             553..585
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(589..597,598..612)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     complement(1371..1389)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1385..1432
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      1529..1984
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1616..1635)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1826..1847
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2010..2114
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2115..2183,2184..2975)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2333..2352)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(3146..3602,1..132)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
       61 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
      121 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
      181 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
      241 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
      301 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
      361 taatgcagga tctcgatccc gcgaaattaa tacgactcac tatagggaga ccacaacggt
      421 ttccctctag aatcaccgag tttattcttg acacctgatg cgatgaatga tataatagga
      481 aagtactgtt ttgtttaact ttaagaaggg gatatacata tgcggggttc tcatcatcat
      541 catcatcatg gtatggctag catgactggt ggacagcaaa tgggtcggga tctgtacgac
      601 gatgacgata aggatccgga tcccctagtc aacggcaacc tgaagatcaa aatgtttatg
      661 aatggcagcg tgaacaagca caaattcaaa tgtgaggcag agggagaagg aaatatccat
      721 agcggcaccc atattatgaa gattaccgtg actgagggag gcccgctgcc ttttgccttc
      781 gacatactgt cccggacctt ctgctatggt cacaaaatgt tcctggacaa cagcgggaat
      841 atcccagact tcttcaaaca gagccttaag gacgggtact cttgggagag agaaacgacc
      901 tacgaggacg gcggagtact gaatagtaaa cagaagcaca ctctgaaggg cgactgcatc
      961 acaacttatt ctgaagttac cggaacattt ccccctaacg gcccagtgat gaaaaaaaag
     1021 accctcggct gggagccctc ctgcgaaatg gtcatccccc gggatggggg tttgatcatg
     1081 tacgataata ttgccctgaa gcttgatgac gatcacggcg ggcacctgac cgggaatttt
     1141 aaggccacat acaagtcaaa taaccccaac atcaacctcc ctaaacacca tttcgtggag
     1201 aaccgcgtgg agatgttaga aggtcgggac ggcggcaatt atgtgaagct gcatgaaagg
     1261 aacaaggccc ggtacagcgc cttggagagc gagctcgcta agaagtagga attcgaagct
     1321 tgatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca
     1381 ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg
     1441 aggaactata tccggatctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     1501 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     1561 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     1621 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     1681 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     1741 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     1801 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     1861 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     1921 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     1981 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     2041 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     2101 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     2161 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     2221 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     2281 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     2341 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     2401 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     2461 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     2521 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     2581 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     2641 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     2701 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     2761 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     2821 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     2881 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     2941 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     3001 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     3061 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     3121 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     3181 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     3241 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     3301 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     3361 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     3421 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     3481 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     3541 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     3601 tc
//
