LOCUS       sequence-19182-e         686 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf99
FEATURES             Location/Qualifiers
     source          1..686
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             351..392
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             402..419
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(442..459)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    448..674
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 tcaccgaatt ccagctgacc accatgaggt ccctgattat agtccttctg ttcccctcta
       61 tcatctactc catgtctatt agacaatgtg agaagactga agaggaaaca tggggattga
      121 aaatagggtt gtgtataatt gccaaagatt tctatcccga aagaactgat tgcagtgttc
      181 atctcccaac tgcaagtgaa ggattgataa ctgaaggcaa tggattcagg gatatacgaa
      241 acaccgataa attatagcaa ttcccgggga tccgaataaa gggtcaagac aattctgcag
      301 atatccagca cagtggcggc cgctcgagtc tagagggccc gcggttcgaa ggtaagccta
      361 tccctaaccc tctcctcggt ctcgattcta cgcgtaccgg tcatcatcac catcaccatt
      421 gagtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
      481 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
      541 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg ggggggtggg
      601 ggtggggcag gacagcaagg gggaggattg ggaagaccat aacaggcatg ctggggatgc
      661 ggtgggctct atggcttctg aggcgg
//
