LOCUS       sequence-191888-        2637 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BB1 with insert: promoter PPOR1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Prielhofer R, Barrero JJ, Steuer S, Gassler T, Zahrl R, Baumann K, 
            Sauer M, Mattanovich D, Gasser B, Marx H
  TITLE     GoldenPiCS: a Golden Gate-derived modular cloning system for applied
            synthetic biology in the yeast Pichia pastoris.
  JOURNAL   BMC Syst Biol. 2017 Dec 8;11(1):123. doi: 10.1186/s12918-017-0492-3.
  PUBMED    29221460
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Syst 
            Biol."; date: "2017-12-8"; volume: "11(1):123. doi"; pages: " 
            10.1186/s12918-017-0492-3"
COMMENT     Sequence Label: BB1_12_pPOR1
FEATURES             Location/Qualifiers
     source          1..2637
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(15..602)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(95..114)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     642..659
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     643..659
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1575..1591)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1575..1591)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      1639..1670
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        1671..1773
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1774..2589
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCLKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1847..1866)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      join(2613..2637,1..3)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgatgc
       61 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
      121 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
      181 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
      241 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
      301 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
      361 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
      421 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
      481 ctgaagccag ttacctcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
      541 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
      601 aagaagatcc tttgattttc taccgaagaa aggcccaccc gtgtaaaacg acggccagtt
      661 tatctagtca gcttgattct agctgatcgt ggaccggaag gtgagccagt gagttgattg
      721 cagtccagtt acgctggagt ctgaggctcg tcctgaatga tatgcggcct cgaattcgaa
      781 gacgcggagc gcattatttg ggtcggctac tgtcattggt tatgatggcc ttaccgagca
      841 cgaaggtggt tggatagacc acaactggaa acagttgtac aaacagattg cattcatttt
      901 tgcttgtatt ggatactcga tggccatcac cgctcttatc tgtttcatcc tcaaccgtat
      961 tccatttttg caactgcgag cttcagaaga ggctgaggag aaaggtatgg atgaggatca
     1021 gattggagag ttcgcttatg actacgtgga agtacgtcgt gattttttgg cttggggatc
     1081 aggcccaaac aatggcttca aggagccgga agttctggat caggtagttc cggttaatga
     1141 tttcagcagt gaccagaatg tgactaatca gaccaacgaa tctgagaagc agtagagtaa
     1201 atatagagat gatatttagt gtattctaat gcttatgtaa tgtattaagc aaaaagttgt
     1261 gtttatgagt tagcatttgt cttagcaaac ataaaattat gtcgacattt gcaacccgca
     1321 tgtctagtgt ttttagatcg atcttcgatg tgtagaataa tgcctccacg tgatgccccg
     1381 cgattttgtt gggtcgcaat gcctccaaca taaacccatc acgtataaaa agccctctta
     1441 accctccccc ctgtttcgtt tgcttcatca cttaacctga actatcaaca tggcgtcttc
     1501 gaattcgcgc gtgatcttac ggcattatac gtatgatcgg tccacgatca gctagattat
     1561 ctagtcagct tgatgtcata gctgtttcct gaggctcaat actgaccatt taaatcatac
     1621 ctgacctcca tagcagaaag tcaaaagcct ccgaccggag gcttttgact tgatcggcac
     1681 gtaagaggtt ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag
     1741 ttatcgagat tttcaggagc taaggaagct aaaatgagcc atattcaacg ggaaacgtct
     1801 tgcttgaagc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct
     1861 cgcgataatg tcgggcaatc aggtgcgaca atctatcgat tgtatgggaa gcccgatgcg
     1921 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg
     1981 gtcaggctaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt
     2041 actcctgatg atgcatggtt actcaccact gcgatcccag ggaaaacagc attccaggta
     2101 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc
     2161 cggttgcatt cgattcctgt ttgtaattgt ccttttaacg gcgatcgcgt atttcgtctc
     2221 gctcaggcgc aatcacgaat gaataacggt ttggttggtg cgagtgattt tgatgacgag
     2281 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactctt gccattctca
     2341 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg
     2401 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt
     2461 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa
     2521 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcacttgat gctcgatgag
     2581 tttttctaat gaggacctaa atgtaatcac ctggctcacc ttcgggtggg cctttct
//
