LOCUS       sequence-191920-        8651 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral expression of hTERT. Used to create immortalized cell 
            lines..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8651)
  AUTHORS   Counter CM, Hahn WC, Wei W, Caddle SD, Beijersbergen RL, Lansdorp 
            PM, Sedivy JM, Weinberg RA
  TITLE     Dissociation among in vitro telomerase activity, telomere 
            maintenance, and cellular immortalization.
  JOURNAL   Proc Natl Acad Sci U S A 1998 Dec 8;95(25):14723-8.
  PUBMED    9843956
REFERENCE   2  (bases 1 to 8651)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A"; date: "1998-12-8"; volume: "95(25)"; pages: 
            "14723-8"
COMMENT     Sequence Label: pBABE-hygro-hTERT
FEATURES             Location/Qualifiers
     source          1..8651
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             7..476
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    541..740
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             941..1357
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1287..1309
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1325..1341
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             1437..4835
                     /codon_start=1
                     /gene="TERT"
                     /product="human telomerase reverse transcriptase, the 
                     catalytic subunit of telomerase"
                     /label=hTERT
                     /note="prevents telomere shortening and allows 
                     postsenescent cells to proliferate beyond crisis (Counter 
                     et al., 1998); also known as EST2"
                     /translation="MPRAPRCRAVRSLLRSHYREVLPLATFVRRLGPQGWRLVQRGDPA
                     AFRALVAQCLVCVPWDARPPPAAPSFRQVSCLKELVARVLQRLCERGAKNVLAFGFALL
                     DGARGGPPEAFTTSVRSYLPNTVTDALRGSGAWGLLLRRVGDDVLVHLLARCALFVLVA
                     PSCAYQVCGPPLYQLGAATQARPPPHASGPRRRLGCERAWNHSVREAGVPLGLPAPGAR
                     RRGGSASRSLPLPKRPRRGAAPEPERTPVGQGSWAHPGRTRGPSDRGFCVVSPARPAEE
                     ATSLEGALSGTRHSHPSVGRQHHAGPPSTSRPPRPWDTPCPPVYAETKHFLYSSGDKEQ
                     LRPSFLLSSLRPSLTGARRLVETIFLGSRPWMPGTPRRLPRLPQRYWQMRPLFLELLGN
                     HAQCPYGVLLKTHCPLRAAVTPAAGVCAREKPQGSVAAPEEEDTDPRRLVQLLRQHSSP
                     WQVYGFVRACLRRLVPPGLWGSRHNERRFLRNTKKFISLGKHAKLSLQELTWKMSVRGC
                     AWLRRSPGVGCVPAAEHRLREEILAKFLHWLMSVYVVELLRSFFYVTETTFQKNRLFFY
                     RKSVWSKLQSIGIRQHLKRVQLRELSEAEVRQHREARPALLTSRLRFIPKPDGLRPIVN
                     MDYVVGARTFRREKRAERLTSRVKALFSVLNYERARRPGLLGASVLGLDDIHRAWRTFV
                     LRVRAQDPPPELYFVKVDVTGAYDTIPQDRLTEVIASIIKPQNTYCVRRYAVVQKAAHG
                     HVRKAFKSHVSTLTDLQPYMRQFVAHLQETSPLRDAVVIEQSSSLNEASSGLFDVFLRF
                     MCHHAVRIRGKSYVQCQGIPQGSILSTLLCSLCYGDMENKLFAGIRRDGLLLRLVDDFL
                     LVTPHLTHAKTFLRTLVRGVPEYGCVVNLRKTVVNFPVEDEALGGTAFVQMPAHGLFPW
                     CGLLLDTRTLEVQSDYSSYARTSIRASLTFNRGFKAGRNMRRKLFGVLRLKCHSLFLDL
                     QVNSLQTVCTNIYKILLLQAYRFHACVLQLPFHQQVWKNPTFFLRVISDTASLCYSILK
                     AKNAGMSLGAKGAAGPLPSEAVQWLCHQAFLLKLTRHRVTYVPLLGSLRTAQTQLSRKL
                     PGTTLTALEAAANPALPSDFKTILD"
     primer_bind     complement(4847..4867)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4852..5181
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5032..5167
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5255..6280
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5255..6280
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     6797..6816
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             7078..7547
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             complement(join(7662..8453,8454..8522))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8285..8304
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8523..8627)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgcagcctga atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag
       61 ggccaagaac agatggaaca gctgaatatg ggccaaacag gatatctgtg gtaagcagtt
      121 cctgccccgg ctcagggcca agaacagatg gtccccagat gcggtccagc cctcagcagt
      181 ttctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct
      241 tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag
      301 ctcaataaaa gagcccacaa cccctcactc ggggcgccag tcctccgatt gactgagtcg
      361 cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct
      421 gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcatttg
      481 ggggctcgtc cgggatcggg agacccctgc ccagggacca ccgacccacc accgggaggc
      541 aagctggcca gcaacttatc tgtgtctgtc cgattgtcta gtgtctatga ctgattttat
      601 gcgcctgcgt cggtactagt tagctaacta gctctgtatc tggcggaccc gtggtggaac
      661 tgacgagttc tgaacacccg gccgcaaccc tgggagacgt cccagggact ttgggggccg
      721 tttttgtggc ccgacctgag gaagggagtc gatgtggaat ccgaccccgt caggatatgt
      781 ggttctggta ggagacgaga acctaaaaca gttcccgcct ccgtctgaat ttttgctttc
      841 ggtttggaac cgaagccgcg cgtcttgtct gctgcagcgc tgcagcatcg ttctgtgttg
      901 tctctgtctg actgtgtttc tgtatttgtc tgaaaattag ggccagactg ttaccactcc
      961 cttaagtttg accttaggtc actggaaaga tgtcgagcgg atcgctcaca accagtcggt
     1021 agatgtcaag aagagacgtt gggttacctt ctgctctgca gaatggccaa cctttaacgt
     1081 cggatggccg cgagacggca cctttaaccg agacctcatc acccaggtta agatcaaggt
     1141 cttttcacct ggcccgcatg gacacccaga ccaggtcccc tacatcgtga cctgggaagc
     1201 cttggctttt gacccccctc cctgggtcaa gccctttgta caccctaagc ctccgcctcc
     1261 tcttcctcca tccgccccgt ctctccccct tgaacctcct cgttcgaccc cgcctcgatc
     1321 ctccctttat ccagccctca ctccttctct aggcgccggc cggatcccag tgtggtggta
     1381 cgtaggaatt cgtcctgctg cgcacgtggg aagccctggc cccggccacc cccgcgatgc
     1441 cgcgcgctcc ccgctgccga gccgtgcgct ccctgctgcg cagccactac cgcgaggtgc
     1501 tgccgctggc cacgttcgtg cggcgcctgg ggccccaggg ctggcggctg gtgcagcgcg
     1561 gggacccggc ggctttccgc gcgctggtgg cccagtgcct ggtgtgcgtg ccctgggacg
     1621 cacggccgcc ccccgccgcc ccctccttcc gccaggtgtc ctgcctgaag gagctggtgg
     1681 cccgagtgct gcagaggctg tgcgagcgcg gcgcgaagaa cgtgctggcc ttcggcttcg
     1741 cgctgctgga cggggcccgc gggggccccc ccgaggcctt caccaccagc gtgcgcagct
     1801 acctgcccaa cacggtgacc gacgcactgc gggggagcgg ggcgtggggg ctgctgctgc
     1861 gccgcgtggg cgacgacgtg ctggttcacc tgctggcacg ctgcgcgctc tttgtgctgg
     1921 tggctcccag ctgcgcctac caggtgtgcg ggccgccgct gtaccagctc ggcgctgcca
     1981 ctcaggcccg gcccccgcca cacgctagtg gaccccgaag gcgtctggga tgcgaacggg
     2041 cctggaacca tagcgtcagg gaggccgggg tccccctggg cctgccagcc ccgggtgcga
     2101 ggaggcgcgg gggcagtgcc agccgaagtc tgccgttgcc caagaggccc aggcgtggcg
     2161 ctgcccctga gccggagcgg acgcccgttg ggcaggggtc ctgggcccac ccgggcagga
     2221 cgcgtggacc gagtgaccgt ggtttctgtg tggtgtcacc tgccagaccc gccgaagaag
     2281 ccacctcttt ggagggtgcg ctctctggca cgcgccactc ccacccatcc gtgggccgcc
     2341 agcaccacgc gggcccccca tccacatcgc ggccaccacg tccctgggac acgccttgtc
     2401 ccccggtgta cgccgagacc aagcacttcc tctactcctc aggcgacaag gagcagctgc
     2461 ggccctcctt cctactcagc tctctgaggc ccagcctgac tggcgctcgg aggctcgtgg
     2521 agaccatctt tctgggttcc aggccctgga tgccagggac tccccgcagg ttgccccgcc
     2581 tgccccagcg ctactggcaa atgcggcccc tgtttctgga gctgcttggg aaccacgcgc
     2641 agtgccccta cggggtgctc ctcaagacgc actgcccgct gcgagctgcg gtcaccccag
     2701 cagccggtgt ctgtgcccgg gagaagcccc agggctctgt ggcggccccc gaggaggagg
     2761 acacagaccc ccgtcgcctg gtgcagctgc tccgccagca cagcagcccc tggcaggtgt
     2821 acggcttcgt gcgggcctgc ctgcgccggc tggtgccccc aggcctctgg ggctccaggc
     2881 acaacgaacg ccgcttcctc aggaacacca agaagttcat ctccctgggg aagcatgcca
     2941 agctctcgct gcaggagctg acgtggaaga tgagcgtgcg gggctgcgct tggctgcgca
     3001 ggagcccagg ggttggctgt gttccggccg cagagcaccg tctgcgtgag gagatcctgg
     3061 ccaagttcct gcactggctg atgagtgtgt acgtcgtcga gctgctcagg tctttctttt
     3121 atgtcacgga gaccacgttt caaaagaaca ggctcttttt ctaccggaag agtgtctgga
     3181 gcaagttgca aagcattgga atcagacagc acttgaagag ggtgcagctg cgggagctgt
     3241 cggaagcaga ggtcaggcag catcgggaag ccaggcccgc cctgctgacg tccagactcc
     3301 gcttcatccc caagcctgac gggctgcggc cgattgtgaa catggactac gtcgtgggag
     3361 ccagaacgtt ccgcagagaa aagagggccg agcgtctcac ctcgagggtg aaggcactgt
     3421 tcagcgtgct caactacgag cgggcgcggc gccccggcct cctgggcgcc tctgtgctgg
     3481 gcctggacga tatccacagg gcctggcgca ccttcgtgct gcgtgtgcgg gcccaggacc
     3541 cgccgcctga gctgtacttt gtcaaggtgg atgtgacggg cgcgtacgac accatccccc
     3601 aggacaggct cacggaggtc atcgccagca tcatcaaacc ccagaacacg tactgcgtgc
     3661 gtcggtatgc cgtggtccag aaggccgccc atgggcacgt ccgcaaggcc ttcaagagcc
     3721 acgtctctac cttgacagac ctccagccgt acatgcgaca gttcgtggct cacctgcagg
     3781 agaccagccc gctgagggat gccgtcgtca tcgagcagag ctcctccctg aatgaggcca
     3841 gcagtggcct cttcgacgtc ttcctacgct tcatgtgcca ccacgccgtg cgcatcaggg
     3901 gcaagtccta cgtccagtgc caggggatcc cgcagggctc catcctctcc acgctgctct
     3961 gcagcctgtg ctacggcgac atggagaaca agctgtttgc ggggattcgg cgggacgggc
     4021 tgctcctgcg tttggtggat gatttcttgt tggtgacacc tcacctcacc cacgcgaaaa
     4081 ccttcctcag gaccctggtc cgaggtgtcc ctgagtatgg ctgcgtggtg aacttgcgga
     4141 agacagtggt gaacttccct gtagaagacg aggccctggg tggcacggct tttgttcaga
     4201 tgccggccca cggcctattc ccctggtgcg gcctgctgct ggatacccgg accctggagg
     4261 tgcagagcga ctactccagc tatgcccgga cctccatcag agccagtctc accttcaacc
     4321 gcggcttcaa ggctgggagg aacatgcgtc gcaaactctt tggggtcttg cggctgaagt
     4381 gtcacagcct gtttctggat ttgcaggtga acagcctcca gacggtgtgc accaacatct
     4441 acaagatcct cctgctgcag gcgtacaggt ttcacgcatg tgtgctgcag ctcccatttc
     4501 atcagcaagt ttggaagaac cccacatttt tcctgcgcgt catctctgac acggcctccc
     4561 tctgctactc catcctgaaa gccaagaacg cagggatgtc gctgggggcc aagggcgccg
     4621 ccggccctct gccctccgag gccgtgcagt ggctgtgcca ccaagcattc ctgctcaagc
     4681 tgactcgaca ccgtgtcacc tacgtgccac tcctggggtc actcaggaca gcccagacgc
     4741 agctgagtcg gaagctcccg gggacgacgc tgactgccct ggaggccgca gccaacccgg
     4801 cactgccctc agacttcaag accatcctgg actgagtcga ccctgtggaa tgtgtgtcag
     4861 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     4921 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     4981 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     5041 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     5101 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     5161 ggaggcctag gcttttgcaa aaagcttgcc agctggggcg ccctctggta aggttgggaa
     5221 gccctgcaga tctgatcccg gggggcaatg agatatgaaa aagcctgaac tcaccgcgac
     5281 gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc
     5341 ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg
     5401 ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc
     5461 ggccgcgctc ccgattccgg aagtgcttga cattggggaa tttagcgaga gcctgaccta
     5521 ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc
     5581 cgctgttctg cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca
     5641 gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga
     5701 tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac
     5761 cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc
     5821 cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg
     5881 ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt
     5941 cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt
     6001 cgagcggagg catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat
     6061 tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc
     6121 gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat
     6181 cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg
     6241 aaaccgacgc cccagcactc gtccgagggc aaaggaatag agtagatgcc gaccgggatc
     6301 tatcgatgcg gcgggtgtgg tggttacgcg taatctgctg cttgcaaaca aaaaaaccac
     6361 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     6421 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     6481 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     6541 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     6601 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     6661 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     6721 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     6781 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     6841 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     6901 ccagcaacgc atcgataaaa taaaagattt tatttagtct ccagaaaaag gggggaatga
     6961 aagaccccac ctgtaggttt ggcaagctag cttaagtaac gccattttgc aaggcatgga
     7021 aaaatacata actgagaata gagaagttca gatcaaggtc aggaacagat ggaacagctg
     7081 aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa
     7141 cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg
     7201 gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag tttctagaga
     7261 accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc ttatttgaac
     7321 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa
     7381 agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc gcccgggtac
     7441 ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc tgttccttgg
     7501 gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcacat gcagcatgta
     7561 tcaaaattaa tttggttttt tttcttaagt atttacatta aatggccata gtgcggccgc
     7621 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     7681 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     7741 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     7801 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     7861 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     7921 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     7981 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     8041 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     8101 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     8161 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     8221 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     8281 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     8341 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     8401 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     8461 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     8521 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     8581 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     8641 aaagtgccac c
//
