LOCUS       sequence-191932-        2882 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BB1 with insert: promoter PRPL2A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Prielhofer R, Barrero JJ, Steuer S, Gassler T, Zahrl R, Baumann K, 
            Sauer M, Mattanovich D, Gasser B, Marx H
  TITLE     GoldenPiCS: a Golden Gate-derived modular cloning system for applied
            synthetic biology in the yeast Pichia pastoris.
  JOURNAL   BMC Syst Biol. 2017 Dec 8;11(1):123. doi: 10.1186/s12918-017-0492-3.
  PUBMED    29221460
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Syst 
            Biol."; date: "2017-12-8"; volume: "11(1):123. doi"; pages: " 
            10.1186/s12918-017-0492-3"
COMMENT     Sequence Label: BB1_12_pRPL2A
FEATURES             Location/Qualifiers
     source          1..2882
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1178..1194)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1178..1194)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      1242..1273
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        1274..1376
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1377..2192
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCLKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1450..1469)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      2216..2243
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(2255..2842)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2335..2354)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(2882,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagttta tctagtcagc ttgattctag ctgatcgtgg accggaaggt
       61 gagccagtga gttgattgca gtccagttac gctggagtct gaggctcgtc ctgaatgata
      121 tgcggcctcg aattcgaaga cgcggagaac aatcatgctc acttactttt gattgtgaac
      181 actctgagtc tgttaagtgc tgttggtgta accttcatta agacaccaga ttcttcagca
      241 ctcatctttc tgttcgcaac aatgttagga gtctcacaaa ttgggttcat aatcatttca
      301 atgaccatgt tatctaacat ttcctccaac caagaatact acaagaacaa gggatcatta
      361 gttggtgtct actcattatt tggatgtgtt ggagtcttaa ttgttaataa gtttggaggt
      421 tggtttggtg actggttcat gcatggtcca tttattctgt tagctctatt taacgtttta
      481 ttctttattg ctttatttgc attgaagggt gttaagaaga acgaatccag agactttcag
      541 tcagttttac aaagctttac tggcacccgt gtaggacgta tgttttccaa caacagacat
      601 gttccaaact ctgacgagtt ttagtattta taggtaaaaa tttttagtat ataggtcaaa
      661 atccaaatta gaagtcaatt agatggatta tttctgaaag tggacctttt gagccccttg
      721 gaggttataa ttaagaagta tttcagagtg tcatgacata ctggagtata atcaattcaa
      781 tatataaatt agtgtaaaga tgttggaagt ctgataatac gataatgtaa ctctccctgg
      841 cacttcaaaa tagaccgaag aagaattaaa tcattcaccc tacgccactc taatcataca
      901 gacaccttcc taacgaagcc ttccataagg tacctttcaa ctaccacctt atgatacctc
      961 acacagcttg cccaataagg cacgtgacca gcactagggt taacccgcga acacaagaac
     1021 cctcatactc cccaaaaaaa aaaaccaata aaaaatttca agtcctctta cagcaagaaa
     1081 cattaacaca acatggcgtc ttcgaattcg cgcgtgatct tacggcatta tacgtatgat
     1141 cggtccacga tcagctagat tatctagtca gcttgatgtc atagctgttt cctgaggctc
     1201 aatactgacc atttaaatca tacctgacct ccatagcaga aagtcaaaag cctccgaccg
     1261 gaggcttttg acttgatcgg cacgtaagag gttccaactt tcaccataat gaaataagat
     1321 cactaccggg cgtatttttt gagttatcga gattttcagg agctaaggaa gctaaaatga
     1381 gccatattca acgggaaacg tcttgcttga agccgcgatt aaattccaac atggatgctg
     1441 atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc
     1501 gattgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg
     1561 ccaatgatgt tacagatgag atggtcaggc taaactggct gacggaattt atgcctcttc
     1621 cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc
     1681 cagggaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg
     1741 atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta
     1801 acggcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg
     1861 gtgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa
     1921 tgcataaact cttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg
     1981 ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa
     2041 tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt
     2101 cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc
     2161 agtttcactt gatgctcgat gagtttttct aatgaggacc taaatgtaat cacctggctc
     2221 accttcgggt gggcctttct gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     2281 agcatcacaa aaatcgatgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     2341 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     2401 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     2461 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     2521 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     2581 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     2641 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     2701 tatttggtat ctgcgctctg ctgaagccag ttacctcgga aaaagagttg gtagctcttg
     2761 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     2821 gcgcagaaaa aaaggatctc aagaagatcc tttgattttc taccgaagaa aggcccaccc
     2881 gt
//
