LOCUS       sequence-19204-e         680 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf127
FEATURES             Location/Qualifiers
     source          1..680
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             491..532
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             542..559
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(582..599)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 gtaccgagct cggatccagt acccttcacc gaattccagc tgaccaccat ggctgattat
       61 ctcaatagtg attatagata caataaagat gtagatttag atttggtcaa attgtttttg
      121 gaaaatggga agccgcacgg aataatgtgt agtattgtac cactatggag aaatgataag
      181 gaaaccatct ttttgatatt gaaaacaatg aactcggatg tcctccaaca tatactaatt
      241 gagtatatga cattcggcga tatccctcta gtggaatatg gaactgtggt aaataaagag
      301 gctatacacg aatactttag aaatattaat attgattctt acacgatgaa atatctacta
      361 aaaaaggaag ggagatgcca tcaattatct cgatgagcaa ttcccgggga tccgaataaa
      421 gggtcaagac aattctgcag atatccagca cagtggcggc cgctcgagtc tagagggccc
      481 gcggttcgaa ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgcgtaccgg
      541 tcatcatcac catcaccatt gagtttaaac ccgctgatca gcctcgactg tgccttctag
      601 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
      661 tcccactgtc ctttcctaat
//
