LOCUS       sequence-192085-        3694 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dsRNA and riboprobe synthesis for Schmidtea mediterranea SoxB2-2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Roberts-Galbraith RH, Brubacher JL, Newmark PA
  TITLE     A functional genomics screen in planarians reveals regulators of 
            whole-brain regeneration.
  JOURNAL   Elife. 2016 Sep 9;5. pii: e17002. doi: 10.7554/eLife.17002.
  PUBMED    27612384
REFERENCE   2  (bases 1 to 3694)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-09-9"; volume: "5. pii: e17002. doi"; pages: " 
            10.7554/eLife.17002"
COMMENT     Sequence Label: pRRG764-SoxB2-2
FEATURES             Location/Qualifiers
     source          1..3694
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      7..595
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     496..515
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     749..766
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(778..825)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        839..857
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        861..879
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     861..878
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        complement(1350..1368)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(1372..1390)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      1404..1451
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             1858..2652
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1912..1931)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2522..2541
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(2670..2738,2739..3530)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2888..2907)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
       61 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
      121 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
      181 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
      241 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
      301 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
      361 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
      421 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
      481 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
      541 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
      601 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
      661 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
      721 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cacgcgtcaa
      781 aaaacccctc aagacccgtt tagaggcccc aaggggttat gctaggcgcg ccggatccta
      841 atacgactca ctatagggag atttaggtga cactatagaa gtgaccttga gagtgacggc
      901 agagttccca ataacttttc cgatgataac aagctcgatc gggaacaaga aggaatcaat
      961 ggtaaattac tccaaggata aactaaaggg atcgtgtatt tatcttttcg gaatattgat
     1021 ttcggttttc gtcttgggcg gtatttgtaa tccggatgat ctttcatgtg aatggctctt
     1081 aggcgtttag cttcatcgat aaatggtcgc ttctgcaatt cagttaaatg cttccattca
     1141 gcacccaggc gtttactgat ttctgaattg tgcatttttg gattttcttg ggccattttt
     1201 ctacgttgac ctctactcca aaccataaat gcattcatcg gtcgtttgac gtggtcttct
     1261 tttattgacg cattgctcga gctgacgttg attttttccg tttcttcaac gatatccgat
     1321 ttgtcttcat cgctgtaacc gaaggtctcc ctttagtgag ggttaattct ccctatagtg
     1381 agtcgtatta ggatccggcg cgcctagcat aaccccttgg ggcctctaaa cgggtcttga
     1441 ggggtttttt gacgcgtcga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     1501 atttaacgcg aattttaaca aaattcaggg cgcaagggct gctaaaggaa gcggaacacg
     1561 tagaaagcca gtccgcagaa acggtgctga ccccggatga atgtcagcta ctgggctatc
     1621 tggacaaggg aaaacgcaag cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg
     1681 cgatagctag actgggcggt tttatggaca gcaagcgaac cggaattgcc agctggggcg
     1741 ccctctggta aggttgggaa gccctgcaaa gtaaactgga tggctttctt gccgccaatg
     1801 atctgatggc gcaggggatc aagatctgat caagagacag gatgaggatc gtttcgcatg
     1861 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     1921 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     1981 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag
     2041 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     2101 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     2161 ctcctgtcat cccaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     2221 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     2281 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     2341 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc
     2401 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     2461 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     2521 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     2581 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     2641 gagttcttct gaattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     2701 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     2761 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     2821 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     2881 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     2941 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     3001 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     3061 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     3121 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     3181 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     3241 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     3301 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     3361 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     3421 gtaagccctc ccgtatcgta gttatctaca cgacggggag ccaggcaact atggatgaac
     3481 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     3541 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     3601 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     3661 actgagcgtc agaccccgta gaaaagatca aagg
//
