LOCUS       sequence-192188-        9403 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express yeast Rcl1 with Y2H activation domain fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9403)
  AUTHORS   Vincent NG, Charette JM, Baserga SJ
  TITLE     The SSU processome interactome in Saccharomyces cerevisiae reveals 
            potential new protein subcomplexes.
  JOURNAL   RNA. 2017 Oct 20. pii: rna.062927.117. doi: 10.1261/rna.062927.117.
  PUBMED    29054886
REFERENCE   2  (bases 1 to 9403)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2017 
            Oct 20. pii: rna.062927.117. doi: 10.1261/rna.062927.117."
COMMENT     Sequence Label: pACT2-Rcl1
FEATURES             Location/Qualifiers
     source          1..9403
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..141
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(67..86)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     227..249
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        278..679
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             725..745
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             761..1102
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     CDS             1115..1141
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1121..1147
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     terminator      2673..2860
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2728..2752)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     protein_bind    complement(2881..2914)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(2992..3025)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(3069..3102)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(3180..3213)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(3257..3290)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(3368..3401)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(3910..5004)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(5005..5410)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     rep_origin      5887..7229
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(6850..6897)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        7494..7598
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7599..7667,7668..8459)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7817..7836)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8630..9218
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9119..9138
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9372..9389
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac
       61 tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta
      121 cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg
      181 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
      241 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agcaacttct tttctttttt
      301 tttcttttct ctctcccccg ttgttgtctc accatatccg caatgacaaa aaaatgatgg
      361 aagacactaa aggaaaaaat taacgacaaa gacagcacca acagatgtcg ttgttccaga
      421 gctgatgagg ggtatctcga agcacacgaa actttttcct tccttcattc acgcacacta
      481 ctctctaatg agcaacggta tacggccttc cttccagtta cttgaatttg aaataaaaaa
      541 aagtttgctg tcttgctatc aagtataaat agacctgcaa ttattaatct tttgtttcct
      601 cgtcattgtt ctcgttccct ttcttccttg tttctttttc tgcacaatat ttcaagctat
      661 accaagcata caatcaactc caagctttgc aaagatggat aaagcggaat taattcccga
      721 gcctccaaaa aagaagagaa aggtcgaatt gggtaccgcc gccaatttta atcaaagtgg
      781 gaatattgct gatagctcat tgtccttcac tttcactaac agtagcaacg gtccgaacct
      841 cataacaact caaacaaatt ctcaagcgct ttcacaacca attgcctcct ctaacgttca
      901 tgataacttc atgaataatg aaatcacggc tagtaaaatt gatgatggta ataattcaaa
      961 accactgtca cctggttgga cggaccaaac tgcgtataac gcgtttggaa tcactacagg
     1021 gatgtttaat accactacaa tggatgatgt atataactat ctattcgatg atgaagatac
     1081 cccaccaaac ccaaaaaaag agatctctat ggcttaccca tacgatgttc cagattacgc
     1141 tagcttgggt ggtcatatgg ccatggaaat gtcatcttcc gcccccaaat acaccacttt
     1201 ccaagggtca caaaatttta ggttgcggat cgtcttgaca acattatcag ggaaaccaat
     1261 aaaaattgaa aaaatccgtt caggcgactt aaatcccggt ctgaaagatt atgaagtgtc
     1321 ttttctaagg ctgatcgagt cggtcaccaa cgggggtgta attgaaattt cttataccgg
     1381 tactactgtg atttataggc ccggtatcat agttggtggt gcatcaactc atatctgccc
     1441 taattccaaa ccggtaggtt atttcgtcga accaatgcta tatttggctc cattttcaaa
     1501 aaagaaattt tctatattat tcaaaggcat aactgcatct cacaacgatg ccggtattga
     1561 agctatcaaa tggggactca tgcccgttat ggaaaaattt ggcgttagag agtgcgctct
     1621 acatactttg aaaagaggtt caccaccact cggaggtggc gaagtgcatt tggttgttga
     1681 ttctttgatt gcgcaaccta taactatgca tgaaatagat aggcccataa tttcatcgat
     1741 tactggtgta gcatactcta ccagagtaag tccgtcgctt gtgaatagca tgatcgatgg
     1801 tgctaagaag gtattgaaaa tctgcaatgc gaagttaaca taacggcaga tgtctggaga
     1861 ggtgaaaatt caggaaagag tccaggctgg ggtattactt tggttgctca gtccaagcag
     1921 aaaggttgga gttattttgc agaagatatc ggtgatgcag gttctatacc tgaagaactt
     1981 ggtgaaaaag ttgcctgtca attattagaa gaaatatcaa aaagtgcagc agttggtaga
     2041 aaccagcttc cattagcaat tgtttacatg gtcatcggga aagaagatat cggcagattg
     2101 agaatcaata aggaacagat agacgaaaga ttcataatcc tcttgagaga tattaagaag
     2161 atctttaata ctgaagtctt tttaaaacca gttgacgaag cggataatga agacatgata
     2221 gctactatca agggtattgg tttcacaaac acaagcaaaa agattgcata gggatccgaa
     2281 ttcgagctcg agagatctat gaatcgtaga tactgaaaaa ccccgcaagt tcacttcaac
     2341 tgtgcatcgt gcaccatctc aatttctttc atttatacat cgttttgcct tcttttatgt
     2401 aactatactc ctctaagttt caatcttggc catgtaacct ctgatctata gaatttttta
     2461 aatgactaga attaatgccc atcttttttt tggacctaaa ttcttcatga aaatatatta
     2521 cgagggctta ttcagaagct ttggacttct tcgccagagg tttggtcaag tctccaatca
     2581 aggttgtcgg cttgtctacc ttgccagaaa tttacgaaaa gatggaaaag ggtcaaatcg
     2641 ttggtagata cgttgttgac acttctaaat aagcgaattt cttatgattt atgattttta
     2701 ttattaaata agttataaaa aaaataagtg tatacaaatt ttaaagtgac tcttaggttt
     2761 taaaacgaaa attcttattc ttgagtaact ctttcctgta ggtcaggttg ctttctcagg
     2821 tatagcatga ggtcgctctt attgaccaca cctctaccgg catgccggtc gagggaccta
     2881 ataacttcgt atagcataca ttatacgaag ttatattaag ggttccggat cgcggccgct
     2941 cgacctgcag ccaagctagc ttggctggac gtaaactcct cttcagacct aataacttcg
     3001 tatagcatac attatacgaa gttatattaa gggttattga atatgatcgg aattggtcga
     3061 gggacctaat aacttcgtat agcatacatt atacgaagtt atattaaggg ttccggatcg
     3121 cggccgctcg acctgcagcc aagctagctt ggctggacgt aaactcctct tcagacctaa
     3181 taacttcgta tagcatacat tatacgaagt tatattaagg gttattgaat atgatcggaa
     3241 ttggtcgagg gacctaataa cttcgtatag catacattat acgaagttat attaagggtt
     3301 ccggatcgcg gccgctcgac ctgcagccaa gctagcttgg ctggacgtaa actcctcttc
     3361 agacctaata acttcgtata gcatacatta tacgaagtta tattaagggt tattgaatat
     3421 gatcggaatt ggtcgactac gtcgttaagg ccgtttctga cagagtaaaa ttcttgaggg
     3481 aactttcacc attatgggaa atggttcaag aaggtattga cttaaactcc atcaaatggt
     3541 caggtcattg agtgtttttt atttgttgta tttttttttt tttagagaaa atcctccaat
     3601 atataaatta ggaatcatag tttcatgatt ttctgttaca cctaactttt tgtgtggtgc
     3661 cctcctcctt gtcaatatta atgttaaagt gcaattcttt ttccttatca cgttgagcca
     3721 ttagtatcaa tttgcttacc tgtattcctt tacatcctcc tttttctcct tcttgataaa
     3781 tgtatgtaga ttgcgtatat agtttcgtct accctatgaa catattccat tttgtaattt
     3841 cgtgtcgttt ctattatgaa tttcatttat aaagtttatg tacaaatatc ataaaaaaag
     3901 agaatctttt taagcaagga ttttcttaac ttcttcggcg acagcatcac cgacttcggt
     3961 ggtactgttg gaaccaccta aatcaccagt tctgatacct gcatccaaaa cctttttaac
     4021 tgcatcttca atggccttac cttcttcagg caagttcaat gacaatttca acatcattgc
     4081 agcagacaag atagtggcga tagggttgac cttattcttt ggcaaatctg gagcagaacc
     4141 gtggcatggt tcgtacaaac caaatgcggt gttcttgtct ggcaaagagg ccaaggacgc
     4201 agatggcaac aaacccaagg aacctgggat aacggaggct tcatcggaga tgatatcacc
     4261 aaacatgttg ctggtgatta taataccatt taggtgggtt gggttcttaa ctaggatcat
     4321 ggcggcagaa tcaatcaatt gatgttgaac cttcaatgta ggaaattcgt tcttgatggt
     4381 ttcctccaca gtttttctcc ataatcttga agaggccaaa acattagctt tatccaagga
     4441 ccaaataggc aatggtggct catgttgtag ggccatgaaa gcggccattc ttgtgattct
     4501 ttgcacttct ggaacggtgt attgttcact atcccaagcg acaccatcac catcgtcttc
     4561 ctttctctta ccaaagtaaa tacctcccac taattctctg acaacaacga agtcagtacc
     4621 tttagcaaat tgtggcttga ttggagataa gtctaaaaga gagtcggatg caaagttaca
     4681 tggtcttaag ttggcgtaca attgaagttc tttacggatt tttagtaaac cttgttcagg
     4741 tctaacacta cctgtacccc atttaggacc acccacagca cctaacaaaa cggcatcagc
     4801 cttcttggag gcttccagcg cctcatctgg aagtggaaca cctgtagcat cgatagcagc
     4861 accaccaatt aaatgatttt cgaaatcgaa cttgacattg gaacgaacat cagaaatagc
     4921 tttaagaacc ttaatggctt cggctgtgat ttcttgacca acgtggtcac ctggcaaaac
     4981 gacgatcttc ttaggggcag acattagaat ggtatatcct tgaaatatat atatatattg
     5041 ctgaaatgta aaaggtaaga aaagttagaa agtaagacga ttgctaacca cctattggaa
     5101 aaaacaatag gtccttaaat aatattgtca acttcaagta ttgtgatgca agcatttagt
     5161 catgaacgct tctctattct atatgaaaag ccggttccgg cgctctcacc tttccttttt
     5221 ctcccaattt ttcagttgaa aaaggtatat gcgtcaggcg acctctgaaa ttaacaaaaa
     5281 atttccagtc atcgaatttg attctgtgcg atagcgcccc tgtgtgttct cgttatgttg
     5341 aggaaaaaaa taatggttgc taagagattc gaactcttgc atcttacgat acctgagtat
     5401 tcccacagtt ccttaccact cttttgttac tctattgatc cagctcagca aaggcagtgt
     5461 gatctaagat tctatcttcg cgatgtagta aaactagcta gaccgagaaa gagactagaa
     5521 atgcaaaagg cacttctaca atggctgcca tcattattat ccgatgtgac gctgcagctt
     5581 ctcaatgata ttcgaatacg ctttgaggag atacagccta atatccgaca aactgtttta
     5641 cagatttacg atcgtacttg ttacccatca ttgaattttg aacatccgaa cctgggagtt
     5701 ttccctgaaa cagatagtat atttgaacct gtataataat atatagtcta gcgctttacg
     5761 gaagacaatg tatgtatttc ggttcctgga gaaactattg catctattgc ataggtaatc
     5821 ttgcacgtcg catccccggt tcattttctg cgtttccatc ttgcacttca atagcatatc
     5881 tttgttaacg aagcatctgt gcttcatttt gtagaacaaa aatgcaacgc gagagcgcta
     5941 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgaaagcg
     6001 ctattttacc aacgaagaat ctgtgcttca tttttgtaaa acaaaaatgc aacgcgagag
     6061 cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga
     6121 gagcgctatt ttaccaacaa agaatctata cttctttttt gttctacaaa aatgcatccc
     6181 gagagcgcta tttttctaac aaagcatctt agattacttt ttttctcctt tgtgcgctct
     6241 ataatgcagt ctcttgataa ctttttgcac tgtaggtccg ttaaggttag aagaaggcta
     6301 ctttggtgtc tattttctct tccataaaaa aagcctgact ccacttcccg cgtttactga
     6361 ttactagcga agctgcgggt gcattttttc aagataaagg catccccgat tatattctat
     6421 accgatgtgg attgcgcata ctttgtgaac agaaagtgat agcgttgatg attcttcatt
     6481 ggtcagaaaa ttatgaacgg tttcttctat tttgtctcta tatactacgt ataggaaatg
     6541 tttacatttt cgtattgttt tcgattcact ctatgaatag ttcttactac aatttttttg
     6601 tctaaagagt aatactagag ataaacataa aaaatgtaga ggtcgagttt agatgcaagt
     6661 tcaaggagcg aaaggtggat gggtaggtta tatagggata tagcacagag atatatagca
     6721 aagagatact tttgagcaat gtttgtggaa gcggtattcg caatatttta gtagctcgtt
     6781 acagtccggt gcgtttttgg ttttttgaaa gtgcgtcttc agagcgcttt tggttttcaa
     6841 aagcgctctg aagttcctat actttctaga gaataggaac ttcggaatag gaacttcaaa
     6901 gcgtttccga aaacgagcgc ttccgaaaat gcaacgcgag ctgcgcacat acagctcact
     6961 gttcacgtcg cacctatatc tgcgtgttgc ctgtatatat atatacatga gaagaacggc
     7021 atagtgcgtg tttatgctta aatgcgtact tatatgcgtc tatttatgta ggatgaaagg
     7081 tagtctagta cctcctgtga tattatccca ttccatgcgg ggtatcgtat gcttccttca
     7141 gcactaccct ttagctgttc tatatgctgc cactcctcaa ttggattagt ctcatccttc
     7201 aatgctatca tttcctttga tattggatca tatgcatagt accgagaaac tagtgcgaag
     7261 tagtgatcag gtattgctgt tatctgatga gtatacgttg tcctggccac ggcagaagca
     7321 cgcttatcgc tccaatttcc cacaacatta gtcaactccg ttaggccctt cattgaaaga
     7381 aatgaggtca tcaaatgtct tccaatgtga gattttgggc cattttttat agcaaagatt
     7441 gaataaggcg catttttctt caaagctcag gtggcacttt tcggggaaat gtgcgcggaa
     7501 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     7561 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     7621 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     7681 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     7741 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     7801 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc
     7861 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     7921 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     7981 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     8041 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     8101 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt
     8161 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     8221 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     8281 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     8341 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     8401 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     8461 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     8521 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     8581 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     8641 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     8701 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     8761 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     8821 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     8881 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     8941 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     9001 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     9061 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     9121 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     9181 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     9241 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     9301 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     9361 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcg
//
