LOCUS       sequence-192362-       17078 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To episomally express codon optimized Cas9 and chimeric guide RNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17078)
  AUTHORS   Li L, Gao F, Wu S
  TITLE     An episomal CRISPR/Cas9 system to derive vector-free gene modified 
            mammalian cells.
  JOURNAL   Protein Cell. 2016 Sep;7(9):689-91. doi: 10.1007/s13238-016-0299-9.
  PUBMED    27472953
REFERENCE   2  (bases 1 to 17078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Protein Cell"; date: "2016-09"; volume: "7"; issue: "9"; pages: 
            "689-91"
COMMENT     Sequence Label: pCRISPR-S12
FEATURES             Location/Qualifiers
     source          1..17078
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        173..552
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        553..756
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     707..727
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          848..980
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     regulatory      1010..1019
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1019..1084
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             1091..1111
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1136..5236
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             1136..5236
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5237..5284
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5294..5359
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             join(5360..5362,5363..5365,5366..6079)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5360..5362,5363..5365,5366..6076)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5666..5685)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6013..6034
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(6104..6121)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6110..6317
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        6372..6620
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     6372..6392
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     6543..6562
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        6642..6717
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        6822..6893
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6895..7252
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6895..7086
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6918..6938)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7103..7238
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7305..7904
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7305..7324)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7801..7821
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    8143..8190
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8661..10450
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(10752..12677)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(13112..13130)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        13198..13302
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(13303..13371,13372..14163)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(13521..13540)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      14334..14922
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14823..14842
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        15353..15498
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             15541..16578
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     polyA_signal    16620..16668
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gtcgattaat taatagggat aacagggtaa ttttgtcaat attggccatt agccatatta
       61 ttcattggtt atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta
      121 tatcataata tgtacattta tattggctca tgtccaatat gaccgccatg ttggcattga
      181 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
      241 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
      301 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
      361 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
      421 catatgccaa gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
      481 gcccagtaca tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc
      541 gctattacca tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac
      601 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
      661 aatcaacggg actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt
      721 aggcgtgtac ggtgggaggt ctatataagc agaggtcgtt tagtgaaccg tcagatcact
      781 agtagcttta ttgcggtagt ttatcacagt taaattgcta acgcagtcag tgctcgactg
      841 atcacaggta agtatcaagg ttacaagaca ggtttaagga ggccaataga aactgggctt
      901 gtcgagacag agaagattct tgcgtttctg ataggcacct attggtctta ctgacatcca
      961 ctttgccttt ctctccacag gggtaccgaa gccgctagtc gacaccggtg ccaccatgga
     1021 ctataaggac cacgacggag actacaagga tcatgatatt gattacaaag acgatgacga
     1081 taagatggcc ccaaagaaga agcggaaggt cggtatccac ggagtcccag cagccgacaa
     1141 gaagtacagc atcggcctgg acatcggcac caactctgtg ggctgggccg tgatcaccga
     1201 cgagtacaag gtgcccagca agaaattcaa ggtgctgggc aacaccgacc ggcacagcat
     1261 caagaagaac ctgatcggag ccctgctgtt cgacagcggc gaaacagccg aggccacccg
     1321 gctgaagaga accgccagaa gaagatacac cagacggaag aaccggatct gctatctgca
     1381 agagatcttc agcaacgaga tggccaaggt ggacgacagc ttcttccaca gactggaaga
     1441 gtccttcctg gtggaagagg ataagaagca cgagcggcac cccatcttcg gcaacatcgt
     1501 ggacgaggtg gcctaccacg agaagtaccc caccatctac cacctgagaa agaaactggt
     1561 ggacagcacc gacaaggccg acctgcggct gatctatctg gccctggccc acatgatcaa
     1621 gttccggggc cacttcctga tcgagggcga cctgaacccc gacaacagcg acgtggacaa
     1681 gctgttcatc cagctggtgc agacctacaa ccagctgttc gaggaaaacc ccatcaacgc
     1741 cagcggcgtg gacgccaagg ccatcctgtc tgccagactg agcaagagca gacggctgga
     1801 aaatctgatc gcccagctgc ccggcgagaa gaagaatggc ctgttcggaa acctgattgc
     1861 cctgagcctg ggcctgaccc ccaacttcaa gagcaacttc gacctggccg aggatgccaa
     1921 actgcagctg agcaaggaca cctacgacga cgacctggac aacctgctgg cccagatcgg
     1981 cgaccagtac gccgacctgt ttctggccgc caagaacctg tccgacgcca tcctgctgag
     2041 cgacatcctg agagtgaaca ccgagatcac caaggccccc ctgagcgcct ctatgatcaa
     2101 gagatacgac gagcaccacc aggacctgac cctgctgaaa gctctcgtgc ggcagcagct
     2161 gcctgagaag tacaaagaga ttttcttcga ccagagcaag aacggctacg ccggctacat
     2221 tgacggcgga gccagccagg aagagttcta caagttcatc aagcccatcc tggaaaagat
     2281 ggacggcacc gaggaactgc tcgtgaagct gaacagagag gacctgctgc ggaagcagcg
     2341 gaccttcgac aacggcagca tcccccacca gatccacctg ggagagctgc acgccattct
     2401 gcggcggcag gaagattttt acccattcct gaaggacaac cgggaaaaga tcgagaagat
     2461 cctgaccttc cgcatcccct actacgtggg ccctctggcc aggggaaaca gcagattcgc
     2521 ctggatgacc agaaagagcg aggaaaccat caccccctgg aacttcgagg aagtggtgga
     2581 caagggcgct tccgcccaga gcttcatcga gcggatgacc aacttcgata agaacctgcc
     2641 caacgagaag gtgctgccca agcacagcct gctgtacgag tacttcaccg tgtataacga
     2701 gctgaccaaa gtgaaatacg tgaccgaggg aatgagaaag cccgccttcc tgagcggcga
     2761 gcagaaaaag gccatcgtgg acctgctgtt caagaccaac cggaaagtga ccgtgaagca
     2821 gctgaaagag gactacttca agaaaatcga gtgcttcgac tccgtggaaa tctccggcgt
     2881 ggaagatcgg ttcaacgcct ccctgggcac ataccacgat ctgctgaaaa ttatcaagga
     2941 caaggacttc ctggacaatg aggaaaacga ggacattctg gaagatatcg tgctgaccct
     3001 gacactgttt gaggacagag agatgatcga ggaacggctg aaaacctatg cccacctgtt
     3061 cgacgacaaa gtgatgaagc agctgaagcg gcggagatac accggctggg gcaggctgag
     3121 ccggaagctg atcaacggca tccgggacaa gcagtccggc aagacaatcc tggatttcct
     3181 gaagtccgac ggcttcgcca acagaaactt catgcagctg atccacgacg acagcctgac
     3241 ctttaaagag gacatccaga aagcccaggt gtccggccag ggcgatagcc tgcacgagca
     3301 cattgccaat ctggccggca gccccgccat taagaagggc atcctgcaga cagtgaaggt
     3361 ggtggacgag ctcgtgaaag tgatgggccg gcacaagccc gagaacatcg tgatcgaaat
     3421 ggccagagag aaccagacca cccagaaggg acagaagaac agccgcgaga gaatgaagcg
     3481 gatcgaagag ggcatcaaag agctgggcag ccagatcctg aaagaacacc ccgtggaaaa
     3541 cacccagctg cagaacgaga agctgtacct gtactacctg cagaatgggc gggatatgta
     3601 cgtggaccag gaactggaca tcaaccggct gtccgactac gatgtggacc atatcgtgcc
     3661 tcagagcttt ctgaaggacg actccatcga caacaaggtg ctgaccagaa gcgacaagaa
     3721 ccggggcaag agcgacaacg tgccctccga agaggtcgtg aagaagatga agaactactg
     3781 gcggcagctg ctgaacgcca agctgattac ccagagaaag ttcgacaatc tgaccaaggc
     3841 cgagagaggc ggcctgagcg aactggataa ggccggcttc atcaagagac agctggtgga
     3901 aacccggcag atcacaaagc acgtggcaca gatcctggac tcccggatga acactaagta
     3961 cgacgagaat gacaagctga tccgggaagt gaaagtgatc accctgaagt ccaagctggt
     4021 gtccgatttc cggaaggatt tccagtttta caaagtgcgc gagatcaaca actaccacca
     4081 cgcccacgac gcctacctga acgccgtcgt gggaaccgcc ctgatcaaaa agtaccctaa
     4141 gctggaaagc gagttcgtgt acggcgacta caaggtgtac gacgtgcgga agatgatcgc
     4201 caagagcgag caggaaatcg gcaaggctac cgccaagtac ttcttctaca gcaacatcat
     4261 gaactttttc aagaccgaga ttaccctggc caacggcgag atccggaagc ggcctctgat
     4321 cgagacaaac ggcgaaaccg gggagatcgt gtgggataag ggccgggatt ttgccaccgt
     4381 gcggaaagtg ctgagcatgc cccaagtgaa tatcgtgaaa aagaccgagg tgcagacagg
     4441 cggcttcagc aaagagtcta tcctgcccaa gaggaacagc gataagctga tcgccagaaa
     4501 gaaggactgg gaccctaaga agtacggcgg cttcgacagc cccaccgtgg cctattctgt
     4561 gctggtggtg gccaaagtgg aaaagggcaa gtccaagaaa ctgaagagtg tgaaagagct
     4621 gctggggatc accatcatgg aaagaagcag cttcgagaag aatcccatcg actttctgga
     4681 agccaagggc tacaaagaag tgaaaaagga cctgatcatc aagctgccta agtactccct
     4741 gttcgagctg gaaaacggcc ggaagagaat gctggcctct gccggcgaac tgcagaaggg
     4801 aaacgaactg gccctgccct ccaaatatgt gaacttcctg tacctggcca gccactatga
     4861 gaagctgaag ggctcccccg aggataatga gcagaaacag ctgtttgtgg aacagcacaa
     4921 gcactacctg gacgagatca tcgagcagat cagcgagttc tccaagagag tgatcctggc
     4981 cgacgctaat ctggacaaag tgctgtccgc ctacaacaag caccgggata agcccatcag
     5041 agagcaggcc gagaatatca tccacctgtt taccctgacc aatctgggag cccctgccgc
     5101 cttcaagtac tttgacacca ccatcgaccg gaagaggtac accagcacca aagaggtgct
     5161 ggacgccacc ctgatccacc agagcatcac cggcctgtac gagacacgga tcgacctgtc
     5221 tcagctggga ggcgacaaaa ggccggcggc cacgaaaaag gccggccagg caaaaaagaa
     5281 aaagggaagt ggcgtgaaac agactttgaa ttttgacctt ctcaagttgg cgggagacgt
     5341 ggagtccaac ccagggccca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     5401 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     5461 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     5521 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
     5581 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     5641 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     5701 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     5761 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     5821 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     5881 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     5941 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
     6001 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     6061 ggacgagctg tacaagtgag aattcctaga gctcgctgat cagcctcgac tgtgccttct
     6121 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     6181 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     6241 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagagaat
     6301 agcaggcatg ctggggagcg gccgcgcctt tttacggttc ctggcctttt gctggccttt
     6361 tgctcacatg tgagggccta tttcccatga ttccttcata tttgcatata cgatacaagg
     6421 ctgttagaga gataattgga attaatttga ctgtaaacac aaagatatta gtacaaaata
     6481 cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa
     6541 tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct
     6601 tgtggaaagg acgaaacacc gattttgttt tgatgtattc agttttagag ctagaaatag
     6661 caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt
     6721 tttgttttag agctagaaat agcaagttaa aataaggcta gtccgttttt agcgcgtgcg
     6781 ccaattctgc agacaaatgg cggatcctat ttttctaaat acattcaaat atgtatccgc
     6841 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg
     6901 cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     6961 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     7021 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     7081 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     7141 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     7201 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc
     7261 aagagacagg atgaggatcg tttcgctcta gagtggccac aaccatgacc gagtacaagc
     7321 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg
     7381 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg
     7441 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
     7501 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
     7561 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
     7621 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
     7681 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
     7741 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
     7801 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
     7861 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgagataaa cgcgggactc
     7921 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca
     7981 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     8041 tcctccagcg cggggatctc atgctggagt tcttcgccca ccctaggggg aggctaactg
     8101 aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga
     8161 ataaaacgca cggtgttggg tcgtttgttc ggcgcgcctc gactctagag gatcgatgcc
     8221 ccgccccgga cgaactaaac ctgactacga catctctgcc ccttcttcgc ggggcagtgc
     8281 atgtaatccc ttcagttggt tggtacaact tgccaactgg gccctgttcc acatgtgaca
     8341 cgggggggga ccaaacacaa aggggttctc tgactgtagt tgacatcctt ataaatggat
     8401 gtgcacattt gccaacactg agtggctttc atcctggagc agactttgca gtctgtggac
     8461 tgcaacacaa cattgccttt atgtgtaact cttggctgaa gctcttacac caatgctggg
     8521 ggacatgtac ctcccagggg cccaggaaga ctacgggagg ctacaccaac gtcaatcaga
     8581 ggggcctgtg tagctaccga taagcggacc ctcaagaggg cattagcaat agtgtttata
     8641 aggccccctt gttaacccta aacgggtagc atatgcttcc cgggtagtag tatatactat
     8701 ccagactaac cctaattcaa tagcatatgt tacccaacgg gaagcatatg ctatcgaatt
     8761 agggttagta aaagggtcct aaggaacagc gatatctccc accccatgag ctgtcacggt
     8821 tttatttaca tggggtcagg attccacgag ggtagtgaac cattttagtc acaagggcag
     8881 tggctgaaga tcaaggagcg ggcagtgaac tctcctgaat cttcgcctgc ttcttcattc
     8941 tccttcgttt agctaataga ataactgctg agttgtgaac agtaaggtgt atgtgaggtg
     9001 ctcgaaaaca aggtttcagg tgacgccccc agaataaaat ttggacgggg ggttcagtgg
     9061 tggcattgtg ctatgacacc aatataaccc tcacaaaccc cttgggcaat aaatactagt
     9121 gtaggaatga aacattctga atatctttaa caatagaaat ccatggggtg gggacaagcc
     9181 gtaaagactg gatgtccatc tcacacgaat ttatggctat gggcaacaca taatcctagt
     9241 gcaatatgat actggggtta ttaagatgtg tcccaggcag ggaccaagac aggtgaacca
     9301 tgttgttaca ctctatttgt aacaagggga aagagagtgg acgccgacag cagcggactc
     9361 cactggttgt ctctaacacc cccgaaaatt aaacggggct ccacgccaat ggggcccata
     9421 aacaaagaca agtggccact cttttttttg aaattgtgga gtgggggcac gcgtcagccc
     9481 ccacacgccg ccctgcggtt ttggactgta aaataagggt gtaataactt ggctgattgt
     9541 aaccccgcta accactgcgg tcaaaccact tgcccacaaa accactaatg gcaccccggg
     9601 gaatacctgc ataagtaggt gggcgggcca agataggggc gcgattgctg cgatctggag
     9661 gacaaattac acacacttgc gcctgagcgc caagcacagg gttgttggtc ctcatattca
     9721 cgaggtcgct gagagcacgg tgggctaatg ttgccatggg tagcatatac tacccaaata
     9781 tctggatagc atatgctatc ctaatctata tctgggtagc ataggctatc ctaatctata
     9841 tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc ctaatttata
     9901 tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata
     9961 tctgggtagt atatgctatc ctaatctgta tccgggtagc atatgctatc ctaatagaga
    10021 ttagggtagt atatgctatc ctaatttata tctgggtagc atatactacc caaatatctg
    10081 gatagcatat gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
    10141 ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
    10201 ggtagtatat gctatcctaa tttatatctg ggtagcatag gctatcctaa tctatatctg
    10261 ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa tctgtatccg
    10321 ggtagcatat gctatcctca tgcatataca gtcagcatat gatacccagt agtagagtgg
    10381 gagtgctatc ctttgcatat gccgccacct cccaaggggg cgtgaatttt cgctgcttgt
    10441 ccttttcctg ctggttgctc ccattcttag gtgaatttaa ggaggccagg ctaaagccgt
    10501 cgcatgtctg attgctcacc aggtaaatgt cgctaatgtt ttccaacgcg agaaggtgtt
    10561 gagcgcggag ctgagtgacg tgacaacatg ggtatgccca attgccccat gttgggagga
    10621 cgaaaatggt gacaagacag atggccagaa atacaccaac agcacgcatg atgtctactg
    10681 gggatttatt ctttagtgcg ggggaataca cggcttttaa tacgattgag ggcgtctcct
    10741 aacaagttac atcactcctg cccttcctca ccctcatctc catcacctcc ttcatctccg
    10801 tcatctccgt catcaccctc cgcggcagcc ccttccacca taggtggaaa ccagggaggc
    10861 aaatctactc catcgtcaaa gctgcacaca gtcaccctga tattgcaggt aggagcgggc
    10921 tttgtcataa caaggtcctt aatcgcatcc ttcaaaacct cagcaaatat atgagtttgt
    10981 aaaaagacca tgaaataaca gacaatggac tcccttagcg ggccaggttg tgggccgggt
    11041 ccaggggcca ttccaaaggg gagacgactc aatggtgtaa gacgacattg tggaatagca
    11101 agggcagttc ctcgccttag gttgtaaagg gaggtcttac tacctccata tacgaacaca
    11161 ccggcgaccc aagttccttc gtcggtagtc ctttctacgt gactcctagc caggagagct
    11221 cttaaacctt ctgcaatgtt ctcaaatttc gggttggaac ctccttgacc acgatgcttt
    11281 ccaaaccacc ctcctttttt gcgcctgcct ccatcaccct gaccccgggg tccagtgctt
    11341 gggccttctc ctgggtcatc tgcggggccc tgctctatcg ctcccggggg cacgtcaggc
    11401 tcaccatctg ggccaccttc ttggtggtat tcaaaataat cggcttcccc tacagggtgg
    11461 aaaaatggcc ttctacctgg agggggcctg cgcggtggag acccggatga tgatgactga
    11521 ctactgggac tcctgggcct cttttctcca cgtccacgac ctctccccct ggctctttca
    11581 cgacttcccc ccctggctct ttcacgtcct ctaccccggc ggcctccact acctcctcga
    11641 ccccggcctc cactacctcc tcgaccccgg cctccactgc ctcctcgacc ccggcctcca
    11701 cctcctgctc ctgcccctcc tgctcctgcc cctcctcctg ctcctgcccc tcctgcccct
    11761 cctgctcctg cccctcctgc ccctcctgct cctgcccctc ctgcccctcc tgctcctgcc
    11821 cctcctgccc ctcctcctgc tcctgcccct cctgcccctc ctcctgctcc tgcccctcct
    11881 gcccctcctg ctcctgcccc tcctgcccct cctgctcctg cccctcctgc ccctcctgct
    11941 cctgcccctc ctgctcctgc ccctcctgct cctgcccctc ctgctcctgc ccctcctgcc
    12001 cctcctgccc ctcctcctgc tcctgcccct cctgctcctg cccctcctgc ccctcctgcc
    12061 cctcctgctc ctgcccctcc tcctgctcct gcccctcctg cccctcctgc ccctcctcct
    12121 gctcctgccc ctcctgcccc tcctcctgct cctgcccctc ctcctgctcc tgcccctcct
    12181 gcccctcctg cccctcctcc tgctcctgcc cctcctgccc ctcctcctgc tcctgcccct
    12241 cctcctgctc ctgcccctcc tgcccctcct gcccctcctc ctgctcctgc ccctcctcct
    12301 gctcctgccc ctcctgcccc tcctgcccct cctgcccctc ctcctgctcc tgcccctcct
    12361 cctgctcctg cccctcctgc tcctgcccct cccgctcctg ctcctgctcc tgttccaccg
    12421 tgggtccctt tgcagccaat gcaacttgga cgtttttggg gtctccggac accatctcta
    12481 tgtcttggcc ctgatcctga gccgcccggg gctcctggtc ttccgcctcc tcgtcctcgt
    12541 cctcttcccc gtcctcgtcc atggttatca ccccctcttc tttgaggtcc actgccgccg
    12601 gagccttctg gtccagatgt gtctcccttc tctcctaggc catttccagg tcctgtacct
    12661 ggcccctcgt cagacatgat tcacactaaa agagatcaat agacatcttt attagacgac
    12721 gctcagtgaa tacagggagt gcagactcct gccccctcca acagcccccc caccctcatc
    12781 cccttcatgg tcgctgtcag acagatccag gtctgaaaat tccccatcct ccgaaccatc
    12841 ctcgtcctca tcaccaatta ctcgcagccc ggaaaactcc cgctgaacat cctcaagatt
    12901 tgcgtcctga gcctcaagcc aggcctcaaa ttcctcgtcc ccctttttgc tggacggtag
    12961 ggatggggat tctcgggacc cctcctcttc ctcttcaagg tcaccagaca gagatgctac
    13021 tggggcaacg gaagaaaagc tgggtgcggc ctgtgaggat cagcttatcg atgataagct
    13081 gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt
    13141 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
    13201 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
    13261 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
    13321 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
    13381 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
    13441 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
    13501 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa
    13561 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
    13621 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
    13681 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
    13741 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
    13801 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca
    13861 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
    13921 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
    13981 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
    14041 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
    14101 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
    14161 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
    14221 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
    14281 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
    14341 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
    14401 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
    14461 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
    14521 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
    14581 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
    14641 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
    14701 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
    14761 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
    14821 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
    14881 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
    14941 tttttacggt tcctggcctt ttgctggcct tgaagctgtc cctgatggtc gtcatctacc
    15001 tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc
    15061 ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg
    15121 tcggccccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct
    15181 gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg
    15241 gagtggtgaa tccgttagcg aggtgccgcc ctgcttcatc cccgtggccc gttgctcgcg
    15301 tttgctggcg gtgtccccgg aagaaatata tttgcatgtc tttagttcta tgatgacaca
    15361 aaccccgccc agcgtcttgt cattggcgaa ttcgaacacg cagatgcagt cggggcggcg
    15421 cggtccgagg tccacttcgc atattaaggt gacgcgtgtg gcctcgaaca ccgagcgacc
    15481 ctgcagcgac ccgcttaaca gcgtcaacag cgtgccgcag atcccggggg gcaatgagat
    15541 atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac
    15601 agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat
    15661 gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat
    15721 cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt
    15781 ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg
    15841 caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat
    15901 gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga
    15961 atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat
    16021 cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag
    16081 ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc
    16141 tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg
    16201 atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct
    16261 tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg
    16321 cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac
    16381 ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga
    16441 gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc
    16501 tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc ggatcgggag
    16561 atgggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac
    16621 ggcaataaaa agacagaata aaacgcacgg gtgttgggtc gtttgttcat aaacgcgggg
    16681 ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg
    16741 cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg
    16801 cagccaacgt cggggcggca ggccctgcca tagccactgg ccccgtgggt tagggacggg
    16861 gtcccccatg gggaatggtt tatggttcgt gggggttatt attttgggcg ttgcgtgggg
    16921 tcaggtccac gactggactg agcagacaga cccatggttt ttggatggcc tgggcatgga
    16981 ccgcatgtac tggcgcgaca cgaacaccgg gcgtctgtgg ctgccaaaca cccccgaccc
    17041 ccaaaaacca ccgcgcggat ttctggcgtg ccaagcta
//
