LOCUS       sequence-192433-       10810 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycyline regulated expression of Mesp2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10810)
  AUTHORS   Lalit PA, Salick MR, Nelson DO, Squirrell JM, Shafer CM, Patel NG, 
            Saeed I, Schmuck EG, Markandeya YS, Wong R, Lea MR, Eliceiri KW, 
            Hacker TA, Crone WC, Kyba M, Garry DJ, Stewart R, Thomson JA, Downs 
            KM, Lyons GE, Kamp TJ
  TITLE     Lineage Reprogramming of Fibroblasts into Proliferative Induced 
            Cardiac Progenitor Cells by Defined Factors.
  JOURNAL   Cell Stem Cell. 2016 Mar 3;18(3):354-67. doi: 
            10.1016/j.stem.2015.12.001. Epub 2016 Feb 11.
  PUBMED    26877223
REFERENCE   2  (bases 1 to 10810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2015.12.001"; journalName: "Cell Stem Cell"; date: 
            "2016-03-3- 3"; volume: "18"; issue: "3"; pages: "354-67"
COMMENT     Sequence Label: pSAM2_mCherry_Mesp2
FEATURES             Location/Qualifiers
     source          1..10810
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             509..517
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     protein_bind    complement(985..1009)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    1079..1540
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1135..1152)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             1545..2255
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYGKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1690..1708)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1958..1977
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    2288..2876
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2341..2361)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2759..2770)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2879..2895)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             3404..3584
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3610..3627)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3616..3840
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3886..4314
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3973..3992)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4183..4204
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4323..4343)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4328..4657
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4508..4643
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4570..4589
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4705..4752
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4771..5145
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5275..5396
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5312..5331)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5366..5385
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5445..5461)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5445..5461)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5458..5480)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5469..5485
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5493..5499,5500..5517,5518..5523))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5538..5559
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5676..5693)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5847..6435)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5927..5946)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6606..7397,7398..7466))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7229..7248
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7467..7571)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7646..7665)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7837..8216
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8218..8416
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8371..8391
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8434..8614
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8661..8786
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9283..9516
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9701..9745
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10043..10160
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    10229..10247
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10266..10284
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10303..10321
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10340..10358
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10377..10395
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10414..10432
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10451..10469
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10488..10506
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     10528..10552
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    10625..10649
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cccgtcggcc ctcgcagccc gccggcccgg cccgtagcac gcgcactacc caggcgacgg
       61 cgccccgacg aacgcgccca gcgcccgcag gcggacagcg gcagagcgcc agcgagcgcg
      121 agaagctgcg catgcgcaca ctcgcccgcg cgctgcaaga actgcgccgc ttcctgccgc
      181 cgtcggtggc acctgcaggc cagagcctga ccaagatcga gacgctgcgc ctggccatcc
      241 gctacatcgg ccacctgtca gccctgctgg gcctcagcga ggacagtctg cggcgcaggc
      301 gccgacggag tgcggacgcg gcgttctctc accgatgccc tcaatgcccc gacggtggca
      361 gcccctcaca ggctcagatg cttggtccta gcctgggatc agccatgagt agtggggtgt
      421 cctgggggtg cccgcctgct tgtcctggac ctctgatctc acctgaaaac cttgggaaca
      481 ggatctccaa cgtggatccc tgggtgacac ctccttattg tccccaaata cagtcaccct
      541 tacaccagtc cctagaaaga gccgctgact cctctccctg ggcaccacct caagcatgtc
      601 ctggcatgca gatgtcccca gagcctagga acaagactgg acactggaca caatccactg
      661 aacctgcaga gctgactaaa gtgtatcaga gtctttctgt gtctccagaa ccctgcctgt
      721 ccctgggaag cccacttctc ctgccccgcc catcatgcca gagactacag cctcagcctc
      781 agcctcagcc tcagtggggc tgctggggcc acgatgcaga ggtgctctcc acctctgagg
      841 atcagggttc cagccctgcc ctccagcttc ctgtggccag ccccaccccc agctcaggcc
      901 tgcagctcag tggctgtcct gaactttggc aggaagacct ggaaggaccc ccactgaata
      961 ttttctacta aaagggcgaa ttcgacccag ctttcttgta caaagtggtt gatgcggccg
     1021 cttcgagcag acatgagtcg acgttatttt ccaccatatt gccgtctttt ggcaatgtga
     1081 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg
     1141 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt
     1201 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca
     1261 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc
     1321 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctcacc tcaagcgtat
     1381 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc
     1441 ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaacgtctag gccccccgaa
     1501 ccacggggac gtggttttcc tttgaaaaac acgatgataa taccatggtg agcaagggcg
     1561 aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct
     1621 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca
     1681 cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc
     1741 tgtcccctca gttcatgtac ggctccaagg cctacgggaa gcaccccgcc gacatccccg
     1801 actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg
     1861 acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca
     1921 aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca
     1981 tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga
     2041 tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct
     2101 acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca
     2161 tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc
     2221 actccaccgg cggcatggac gagctgtaca agtaagtcga ccataattcg atatcaagct
     2281 tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     2341 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     2401 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     2461 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     2521 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     2581 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     2641 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     2701 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     2761 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     2821 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
     2881 ataccgtcga cctcgtcgag acctagaaaa acatggagca atcacaagta gcaatacagc
     2941 agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc
     3001 agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     3061 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatat
     3121 ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga actacacacc
     3181 agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
     3241 gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac accctgtgag
     3301 cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg acagccgcct
     3361 agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc tctggttaga
     3421 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     3481 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     3541 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc gtttaaaccc
     3601 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     3661 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     3721 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggcg gggcaggaca
     3781 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     3841 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     3901 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     3961 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     4021 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     4081 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     4141 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     4201 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     4261 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
     4321 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     4381 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     4441 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     4501 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     4561 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
     4621 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
     4681 cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata
     4741 gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg
     4801 ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc
     4861 cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc
     4921 agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc
     4981 ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc
     5041 gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac
     5101 ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat
     5161 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     5221 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
     5281 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     5341 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     5401 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     5461 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     5521 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     5581 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     5641 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     5701 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     5761 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     5821 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     5881 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     5941 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     6001 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     6061 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     6121 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     6181 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     6241 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     6301 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     6361 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     6421 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     6481 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     6541 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     6601 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     6661 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     6721 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     6781 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     6841 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     6901 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     6961 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     7021 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     7081 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     7141 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     7201 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     7261 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     7321 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     7381 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     7441 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     7501 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     7561 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc
     7621 ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
     7681 atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta
     7741 caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg
     7801 cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt
     7861 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
     7921 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
     7981 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
     8041 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
     8101 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
     8161 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
     8221 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
     8281 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
     8341 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
     8401 aggtctatat aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga
     8461 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     8521 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     8581 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
     8641 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
     8701 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
     8761 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
     8821 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
     8881 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
     8941 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     9001 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     9061 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     9121 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     9181 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     9241 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     9301 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     9361 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     9421 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     9481 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     9541 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     9601 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     9661 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     9721 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     9781 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     9841 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     9901 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     9961 cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac
    10021 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
    10081 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
    10141 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttac
    10201 gcgtccccga aaagtgccac ctgacgtctc cctatcagtg atagagaagt cgacacgtct
    10261 cgagctccct atcagtgata gagaaggtac gtctagaacg tctccctatc agtgatagag
    10321 aagtcgacac gtctcgagct ccctatcagt gatagagaag gtacgtctag aacgtctccc
    10381 tatcagtgat agagaagtcg acacgtctcg agctccctat cagtgataga gaaggtacgt
    10441 ctagaacgtc tccctatcag tgatagagaa gtcgacacgt ctcgagctcc ctatcagtga
    10501 tagagaaggt accccctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag
    10561 acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca gcctgctagc
    10621 atcaacaagt ttgtacaaaa aagcaggctc cgaattcgcc cttcagccat ggcccagtcg
    10681 cctcctcctc agagcctcca gggtctcgac cactgggtct tctcccaggg ctggggctgg
    10741 gctcagcaat cggactccac gtctccggcc tcgtcctcag attcgtccgg ttcctgccct
    10801 tgctacgcca
//
