LOCUS       sequence-19255-e         705 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf178
FEATURES             Location/Qualifiers
     source          1..705
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             366..407
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             417..434
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(457..474)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    463..693
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 agtacccttc accgaattcc agctgaccac catggtggat gctataaccg ttctaactgc
       61 gataggcata actgtattaa tgcttttgat ggtaatttct ggtgccgccc tgatagtcaa
      121 ggagttaaat cctaatgata tattcactat gcaatcatta aagtttaatc gagccgtaac
      181 gattttcaaa tatataggac tctttatcta tataccagga acaatcattt tgtacgctac
      241 gtacgtcaaa tccctattaa tgaaaagtta agcaattccc ggggatccga ataaagggtc
      301 aagacaattc tgcagatatc cagcacagtg gcggccgctc gagtctagag ggcccgcggt
      361 tcgaaggtaa gcctatccct aaccctctcc tcggtctcga ttctacgcgt accggtcatc
      421 atcaccatca ccattgagtt taaacccgct gatcagcctc gactgtgcct tctagttgcc
      481 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
      541 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
      601 ttctgggggg gtgggggtgg gggcaggaca gcaagggggg aggaattggg aagacaatag
      661 caggcatgct gggggatgcg gtgggctcta tggcttctga ggcgg
//
