LOCUS       sequence-192592-        5882 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of secretory immunoglobulin heavy chains in mammalian 
            cells, human IgG3 isotype.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5882)
  TITLE     Immunoglobulin expression vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: AbVec2.0-IGHG3
FEATURES             Location/Qualifiers
     source          1..5882
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    99..120
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(135..140,141..158,159..165)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    173..189
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     178..200
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     197..213
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     197..213
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        242..621
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        622..825
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     776..796
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     822..846
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        1056..1074
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1056..1073
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     1087..1106
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      2410..2419
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2423..2557
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2460..2479)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2514..2533
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        2626..2983
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2626..2817
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2649..2669)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2834..2969
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3003..3020)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3003..3019)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3012..3034)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3232..3687
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3319..3338)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3529..3550
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3704..3723)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3823..3845
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3883..3901)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3969..4073
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4074..4142,4143..4934)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4292..4311)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5105..5693
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5594..5613
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5847..5864
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatccaactg gcacgacagg
       61 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
      121 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
      181 ggataacaat ttcacacagg aaacagctat gacatgatta cgaattaatt cgagctcgcc
      241 cgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
      301 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
      361 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
      421 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
      481 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
      541 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
      601 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
      661 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
      721 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
      781 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt
      841 cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga
      901 tccagcctcc gcggccggga acggtgcatt ggaacgcgga ttccccgtgc caagagtgac
      961 gtaagtaccg cctatagagt ctataggccc acccccttgg cttcgttaga acgcggctac
     1021 aattaataca taaccttatg tatcatacac atacgattta ggtgacacta tagaataaca
     1081 tccactttgc ctttctctcc acaggtgtcc actcccaggt ccaactgcac ctcggttcta
     1141 tcgattgaat tccaccatgg gatggtcatg tatcatcctt tttctagtag caactgcaac
     1201 cggttgacta actagctggt cgaccaaggg cccaagcgtc ttccccctgg cgccctgctc
     1261 caggagcacc tctgggggca cagcggccct gggctgcctg gtcaaggact acttcccaga
     1321 gccggtgacg gtgtcgtgga actcaggcgc cctgaccagc ggcgtgcaca ccttcccggc
     1381 tgtcctacag tcctcaggac tctactccct cagcagcgtg gtgaccgtgc cctccagcag
     1441 cttgggcacc cagacctaca cctgcaacgt gaatcacaag cccagcaaca ccaaggtgga
     1501 caagagagtt gagctgaaaa caccactggg cgatacaaca cacacttgcc caagatgccc
     1561 tgaaccaaaa tcttgcgaca cacctccgcc ttgtccacga tgtcctgagc caaagagttg
     1621 cgatacccct cctccttgtc cccggtgtcc cgaacccaag tcctgtgata cgcctccacc
     1681 atgtcccaga tgtccagcac ctgaactcct gggaggaccg tcagtcttcc tcttcccccc
     1741 aaaacccaag gataccctta tgatttcccg gacccctgag gtcacgtgcg tggtggtgga
     1801 cgtgagccac gaagaccccg aggtccagtt caagtggtac gtggacggcg tggaggtgca
     1861 taatgccaag acaaagccgc gggaggagca gtacaacagc acgttccgtg tggtcagcgt
     1921 cctcaccgtc ctgcaccagg actggctgaa cggcaaggag tacaagtgca aggtctccaa
     1981 caaagccctc ccagccccca tcgagaaaac catctccaaa accaaaggac agccccgaga
     2041 accacaggtg tacaccctgc ccccatcccg ggaggagatg accaagaacc aggtcagcct
     2101 gacctgcctg gtcaaaggct tctaccccag cgacatcgcc gtggagtggg agagcagcgg
     2161 gcagccggag aacaactaca acaccacgcc tcccatgctg gactccgacg gctccttctt
     2221 cctctacagc aagctcaccg tggacaagag caggtggcag caggggaaca tcttctcatg
     2281 ctccgtgatg catgaggctc tgcacaaccg cttcacgcag aagagcctct ccctgtctcc
     2341 gggtaaatga gtgcgacggc cggcaagccc ccgctccccg ggctctcggg gtcgcgcgag
     2401 gaaagcttgg ccgccatggc ccaacttgtt tattgcagct tataatggtt acaaataaag
     2461 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     2521 gtccaaactc atcaatgtat cttatcatgt ctggatcggg aattaattcg gcgcagcacc
     2581 atggcctgaa ataacctctg aaagaggaac ttggttaggt accttctgag gcggaaagaa
     2641 ccatctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     2701 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     2761 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     2821 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     2881 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     2941 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctgt taacagcttg
     3001 gcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     3061 cgccttgcag cacatccccc tttcgccagt tggcgtaata gcgaagaggc ccgcaccgat
     3121 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc
     3181 cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct
     3241 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     3301 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     3361 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     3421 ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct
     3481 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     3541 tccaaactgg aacaacactc aaccctatct cgggctattc ttttgattta taagggattt
     3601 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     3661 ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg
     3721 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     3781 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     3841 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     3901 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     3961 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     4021 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     4081 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     4141 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     4201 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     4261 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     4321 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     4381 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     4441 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     4501 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     4561 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     4621 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     4681 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     4741 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     4801 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     4861 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     4921 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     4981 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     5041 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     5101 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     5161 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     5221 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     5281 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     5341 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     5401 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     5461 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     5521 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     5581 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     5641 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     5701 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     5761 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     5821 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     5881 ca
//
