LOCUS       sequence-192694-       13334 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses LCOR, P2A, HOXA9, T2A and HOXA5 in mammalian cells. 
            *Please see notes below on P2A cleaving efficiency..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13334)
  AUTHORS   Sugimura R, Jha DK, Han A, Soria-Valles C, da Rocha EL, Lu YF, 
            Goettel JA, Serrao E, Rowe RG, Malleshaiah M, Wong I, Sousa P, Zhu 
            TN, Ditadi A, Keller G, Engelman AN, Snapper SB, Doulatov S, Daley 
            GQ
  TITLE     Haematopoietic stem and progenitor cells from human pluripotent stem
            cells.
  JOURNAL   Nature. 2017 May 17. doi: 10.1038/nature22370.
  PUBMED    28514439
REFERENCE   2  (bases 1 to 13334)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2017 May 17. doi: 10.1038/nature22370."
COMMENT     Sequence Label: pINDUCER-21-LCOR-P2A-HOXA9-T2A-HOXA5
FEATURES             Location/Qualifiers
     source          1..13334
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..19
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    43..61
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    85..103
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    127..145
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        178..216
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     213..237
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    346..370
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1900..1917
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2467..2520
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     protein_bind    complement(3347..3371)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3424..3450
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        3670..4848
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3901..4839
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4796..4816
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    4918..4951
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(4958..5575,5576..5581,5582..5617,5618..5620,5621..5656,
                     5657..5659,5660..5695,5696..5704)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    5742..6317
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5911..5928)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6138..6157
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(6333..6335,6336..6338,6339..7052)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6333..6335,6336..6338,6339..7049)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6378..6399)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6639..6658)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6986..7007
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     7188..7207
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    7495..8083
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7548..7568)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7966..7977)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8158..8391
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8510..8528)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8596..8700
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8701..8769,8770..9561)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8919..8938)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9732..10320
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10221..10240
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10474..10491
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10731..10752
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10767..10772,10773..10790,10791..10797)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10805..10821
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10810..10832
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10829..10845
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10829..10845
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10895..10914
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             11106..11739
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    11786..11911
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    12408..12641
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12826..12870
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    13163..13280
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
       61 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
      121 taccactccc tatcagtgat agagaaaagt gaaagtcgag ctcggtaccc gggtcgaggt
      181 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      241 tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg atccagcctc
      301 cgcggccccg aactagtcca gtgtggtgga attctgcaga tatcaacaag tttgtacaaa
      361 aaagcaggct ccgcggccgc ccccttcacc atgcagcgaa tgatccaaca atttgctgct
      421 gaatatacct caaaaaatag ctctactcag gaccccagcc agcccaatag cacaaagaac
      481 caaagcctgc cgaaagcatc tccagtcacc acctctccca cggctgcaac tactcagaac
      541 cctgtgctca gcaaacttct catggctgac caagactcac ctctggacct tactgtcaga
      601 aagtctcagt cagaacctag cgaacaagac ggtgtacttg atctgtccac taagaaaagt
      661 ccatgtgctg gcagcacttc cctgagccac tctccaggct gctccagtac tcaagggaac
      721 gggcgacctg ggagacccag ccagtaccgc ccagacggac ttcggagtgg tgatggggta
      781 cctccaagaa gcttacagga tggaaccagg gaaggttttg gacactccac atcactcaaa
      841 gttccactgg ctcgatccct gcagattagt gaagaactac tgagcagaaa ccaattgtcc
      901 acagctgcca gccttgggcc atctggatta cagaatcatg gacaacactt aatattatcc
      961 agggaagcct cttgggcaaa accacattac gagttcaacc tcagccgtat gaagttcagg
     1021 ggaaatggtg cactcagcaa catcagtgac cttccttttc ttgcagaaaa ctctgccttt
     1081 ccaaaaatgg cacttcaagc aaaacaagat ggaaaaaagg atgtgagcca ttcatctcct
     1141 gtagatttaa agataccaca agttcgagga atggatcttt cttgggagtc tcgcactggt
     1201 gatcagtaca gctatagctc tttggtaatg ggttcacaaa cggagagcgc gcttagtaaa
     1261 aaattaaggg ctattcttcc aaaacaaagt agaaaaagca tgttagatgc tggacccgat
     1321 tcttggggct cagatgctga gcagtctacc tctggacagc catatcccac atcggatcaa
     1381 gaaggagacc ctggctccaa gcagcctcgg aagaaaagag ggcgttacag acagtacaac
     1441 agtgagatac tggaggaagc aatctcagtg gttatgagtg gaaaaatgag tgtttccaaa
     1501 gctcagagta tttatgggat tccccacagt acactggagt acaaagtaaa ggagaggctg
     1561 ggcactttga aaaaccctcc aaagaaaaag atgaaattaa tgagtggagc cacgaagcaa
     1621 gcaggagatg ttgaagaaaa ccccgggcct atggccacca ctggggccct gggcaactac
     1681 tacgtggact cgttcctgct gggcgccgac gccgcggatg agctgagcgt tggccgctat
     1741 gcgccgggga ccctgggcca gcctccccgg caggcggcga cgctggccga gcaccccgac
     1801 ttcagcccgt gcagcttcca gtccaaggcg acggtgtttg gcgcctcgtg gaacccagtg
     1861 cacgcggcgg gcgccaacgc tgtacccgct gcggtgtacc accaccatca ccaccacccc
     1921 tacgtgcacc cccaggcgcc cgtggcggcg gcggcgccgg acggcaggta catgcgctcc
     1981 tggctggagc ccacgcccgg tgcgctctcc ttcgcgggct tgccctccag ccggccttat
     2041 ggcattaaac ctgaaccgct gtcggccaga aggggtgact gtcccacgct tgacactcac
     2101 actttgtccc tgactgacta tgcttgtggt tctcctccag ttgatagaga aaaacaaccc
     2161 agcgaaggcg ccttctctga aaacaatgct gagaatgaga gcggcggaga caagcccccc
     2221 atcgatccca ataacccagc agccaactgg cttcatgcgc gctccactcg gaaaaagcgg
     2281 tgcccctata caaaacacca gaccctggaa ctggagaaag agtttctgtt caacatgtac
     2341 ctcaccaggg accgcaggta cgaggtggct cgactgctca acctcaccga gaggcaggtc
     2401 aagatctggt tccagaaccg caggatgaaa atgaagaaaa tcaacaaaga ccgagcaaaa
     2461 gacgaggagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tcccggccct
     2521 atgagctctt attttgtaaa ctcattttgc ggtcgctatc caaatggccc ggactaccag
     2581 ttgcataatt atggagatca tagttccgtg agcgagcaat tcagggactc ggcgagcatg
     2641 cactccggca ggtacggcta cggctacaat ggcatggatc tcagcgtcgg ccgctcgggc
     2701 tccggccact ttggctccgg agagcgcgcc cgcagctacg ctgccagcgc cagcgcggcg
     2761 cccgccgagc ccaggtacag ccagccggcc acgtccacgc actctcctca gcccgatccg
     2821 ctgccctgct ccgccgtggc cccctcgccc ggcagcgaca gccaccacgg cgggaaaaac
     2881 tccctaagca actccagcgg cgcctcggcc gacgccggca gcacccacat cagcagcaga
     2941 gagggggttg gcacggcgtc cggagccgag gaggacgccc ctgccagcag cgagcaggcg
     3001 agtgcgcaga gcgagccgag cccggcgccg cccgcccaac cccagatcta cccctggatg
     3061 cgcaagctgc acataagtca tgacaacata ggcggcccgg aaggcaaaag ggcccggacg
     3121 gcctacacgc gctaccagac cctggagctg gagaaggagt tccacttcaa ccgttacctg
     3181 acccgcagaa ggaggattga aatagcacat gctctttgcc tctccgagag acaaattaaa
     3241 atctggttcc aaaaccggag aatgaagtgg aaaaaagata ataagctgaa aagcatgagc
     3301 atggccgcgg caggaggggc cttccgtccc aagggtgggc gcgccgaccc agctttcttg
     3361 tacaaagtgg ttgatatcca gcacagtggc ggccgctcga gtctagaggg cccgcggttc
     3421 gaatacccat acgacgtccc agactacgct tagtaatgat taattaaact agaaattcta
     3481 ccgggtaggg gaggcgcttt tcccaaggca gtctggagta acccacccaa gatctggcct
     3541 ccgcgccggg ttttggcgcc tcccgcgggc gcccccctcc tcacggcgag ccgcgtaaga
     3601 tctggcctcc gcgccgggtt ttggcgcctc ccgcgggcgc ccccctcctc acggcgagcc
     3661 tagtcgtgag gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     3721 aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac
     3781 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     3841 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
     3901 gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg
     3961 ccttgaatta cttccacctg gctgcagtac gtgattcttg atcccgagct tcgggttgga
     4021 agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt
     4081 gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt
     4141 ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt
     4201 tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt
     4261 tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg
     4321 ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct
     4381 ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg
     4441 tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca
     4501 aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg
     4561 gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg
     4621 cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt
     4681 tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac
     4741 ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag
     4801 cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgaag cggccgccat
     4861 gctagtaaat tgtccgctaa attctggccg tttttggctt ttttgttaga cggatccata
     4921 acttcgtata atgtatgcta tacgaagtta tgccaccatg tctagactgg acaagagcaa
     4981 agtcataaac ggagctctgg aattactcaa tggtgtcggt atcgaaggcc tgacgacaag
     5041 gaaactcgct caaaagctgg gagttgagca gcctaccctg tactggcacg tgaagaacaa
     5101 gcgggccctg ctcgatgccc tgccaatcga gatgctggac aggcatcata cccacttctg
     5161 ccccctggaa ggcgagtcat ggcaagactt tctgcggaac aacgccaagt cataccgctg
     5221 tgctctcctc tcacatcgcg acggggctaa agtgcatctc ggcacccgcc caacagagaa
     5281 acagtacgaa accctggaaa atcagctcgc gttcctgtgt cagcaaggct tctccctgga
     5341 gaacgcactg tacgctctgt ccgccgtggg ccactttaca ctgggctgcg tattggagga
     5401 acaggagcat caagtagcaa aagaggaaag agagacacct accaccgatt ctatgccccc
     5461 acttctgaga caagcaattg agctgttcga ccggcaggga gccgaacctg ccttcctttt
     5521 cggcctggaa ctaatcatat gtggcctgga gaaacagcta aagtgcgaaa gcggcgggcc
     5581 gaccgacgcc cttgacgatt ttgacttaga catgctccca gccgatgccc ttgacgactt
     5641 tgaccttgat atgctgcctg ctgacgctct tgacgatttt gaccttgaca tgctccccgg
     5701 gtaactaagt aaggatccgc ggccgcacta gaggaattcc gcccctctcc cccccccccc
     5761 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt
     5821 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct
     5881 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga
     5941 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga
     6001 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac
     6061 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag
     6121 ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc
     6181 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg
     6241 tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt
     6301 gaaaaacacg atgataagct taccggtcca ccatggtgag caagggcgag gagctgttca
     6361 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     6421 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     6481 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
     6541 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     6601 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     6661 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     6721 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     6781 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     6841 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     6901 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
     6961 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     7021 tcactctcgg catggacgag ctgtacaagt aagcatgccc gacggcgagg atctcgtcgt
     7081 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     7141 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     7201 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     7261 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgatg
     7321 tacaagtaaa gcggccgcga ctctagatca taatcagcca taccacattt gtagaggttt
     7381 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     7441 ttgttgttgt ttagtccctc ccaattcgat atcaagctta tcgcgataga tcctaatcaa
     7501 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     7561 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     7621 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     7681 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     7741 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     7801 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     7861 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     7921 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     7981 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     8041 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctgagat cctttaagac
     8101 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     8161 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     8221 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     8281 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     8341 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt
     8401 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     8461 ggcccgggtt aattaaggaa agggctagat cattcttgaa gacgaaaggg cctcgtgata
     8521 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     8581 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     8641 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     8701 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     8761 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     8821 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     8881 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     8941 cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     9001 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     9061 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     9121 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     9181 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     9241 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     9301 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     9361 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     9421 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     9481 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     9541 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     9601 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     9661 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     9721 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     9781 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     9841 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     9901 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     9961 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
    10021 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
    10081 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
    10141 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
    10201 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
    10261 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
    10321 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
    10381 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
    10441 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
    10501 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagcaag
    10561 ctcatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
    10621 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctccccg
    10681 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
    10741 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
    10801 ggaattgtga gcggataaca atttcacaca ggaaacagct atgacatgat tacgaatttc
    10861 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
    10921 tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc
    10981 accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat
    11041 tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta
    11101 gtagttggaa gggctaattc actcccaaag aagacaagat atccttgatc tgtggatcta
    11161 ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata
    11221 tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga
    11281 ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga
    11341 cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc
    11401 ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact
    11461 ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct
    11521 cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga
    11581 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
    11641 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
    11701 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
    11761 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
    11821 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
    11881 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
    11941 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
    12001 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
    12061 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
    12121 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
    12181 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
    12241 acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
    12301 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
    12361 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
    12421 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
    12481 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
    12541 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
    12601 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
    12661 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
    12721 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
    12781 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
    12841 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
    12901 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
    12961 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
    13021 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
    13081 agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg ccgaattcac
    13141 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
    13201 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
    13261 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggacta
    13321 ggatccttta ccac
//
