LOCUS       sequence-19270-e         603 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf193
FEATURES             Location/Qualifiers
     source          1..603
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             369..410
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             420..437
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(460..477)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    466..577
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 agtacccttc accgaattcc agctgaccac catgaatata tattgcagat attcatcact
       61 aacattggca aatttcttat gtctattgat gaagctctct atatcgtgtc caaatagtct
      121 agaattaaaa tacttggata ttttctgttg gaaagtagtc aacggaagag tgaccacatt
      181 atcgtacaat atatatccct ttttggagag gtccgtcatt aatggaatat gctttacaga
      241 gctagatact aacagagtac ctattttgag atgggcaatt cccggggatc cgaataaagg
      301 gtcaagacaa ttctgcagat atccagcaca gtggcggccg ctcgagtcta gagggcccgc
      361 ggttcgaagg taagcctatc cctaaccctc tcctcggtct cgattctacg cgtaccggtc
      421 atcatcacca tcaccattga gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
      481 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
      541 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
      601 cta
//
