LOCUS       sequence-192888-        1940 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  part designed to occupy position 11 of EMMA. Functional category: 
            polyadenylation signal sequence .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016."
COMMENT     Sequence Label: YCe1889 HC_Kan_SV40-polyA_p11
FEATURES             Location/Qualifiers
     source          1..1940
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      40..71
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        72..174
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             175..990
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(248..267)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1011..1038
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1050..1637)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1130..1149)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1659..1688)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     polyA_signal    1784..1905
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1795..1814)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1849..1868
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 tactgaccat ttaaatcata cctgacctcc atagcagaaa gtcaaaagcc tccgaccgga
       61 ggcttttgac ttgatcggca cgtaagaggt tccaactttc accataatga aataagatca
      121 ctaccgggcg tattttttga gttatcgaga ttttcaggag ctaaggaagc taaaatgagc
      181 catattcaac gggaaacgtc ttgctcgagg ccgcgattaa attccaacat ggatgctgat
      241 ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcga
      301 ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc
      361 aatgatgtta cagatgagat ggtcaggcta aactggctga cggaatttat gcctcttccg
      421 accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac tgcgatccca
      481 gggaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat
      541 gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac
      601 ggcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttggt
      661 gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg
      721 cataagcttt tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat
      781 aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc
      841 gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca
      901 ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag
      961 tttcacttga tgctcgatga gtttttctga gggcccaaat gtaatcacct ggctcacctt
     1021 cgggtgggcc tttctgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     1081 cacaaaaatc gatgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     1141 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     1201 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     1261 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     1321 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     1381 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     1441 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     1501 ggtatctgcg ctctgctgaa gccagttacc tcggaaaaag agttggtagc tcttgatccg
     1561 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     1621 gaaaaaaagg atctcaagaa gatcctttga ttttctaccg aagaaaggcc cacccgtgaa
     1681 ggtgagccag tgagttgatt gcagtccagt tacgctggag tctgaggctc gtcctgaatg
     1741 atatcaagct tgaattcgtt ttcgtctcgg acgacagaca tgataagata cattgatgag
     1801 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     1861 gctattgctt tatttgtaac cattataagc tgcaataaac aagttacccg agacgtacga
     1921 attctctaga tatcgctcaa
//
