LOCUS       sequence-19291-e         767 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf214
FEATURES             Location/Qualifiers
     source          1..767
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             491..532
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             542..559
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(582..599)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    588..699
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gagctcggat ccgtaccctt caccgaattc cagctgacca ccatgataac tttattttta
       61 atcctatgtt atttcattct tatttttaat attatagtac ctgcaatatc tgaaaaaatg
      121 agacgcgaac gagccgcata cgtaaactac aaacgtctaa acaagaattt catttgtgtc
      181 gatgatagac tgtttagtta taattttaca acatctggaa ttaaggcaaa ggtggccgtt
      241 gataacaaaa atgttcccat tccatgttcc aagataaacg aggtcaataa taataaagat
      301 gtcgatacac tatattgtga taaagataga gacgatatac caggttttgc acgatcgtgt
      361 tatagggcat attctgactt attttttact acctaagcaa ttcccgggga tccgaataaa
      421 gggtcaagac aattctgcag atatccagca cagtggcggc cgctcgagtc tagagggccc
      481 gcggttcgaa ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgcgtaccgg
      541 tcatcatcac catcaccatt gagtttaaac ccgctgatca gcctcgactg tgccttctag
      601 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
      661 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
      721 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggatt
//
