LOCUS       sequence-19294-e         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf217
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             768..809
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             819..836
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gagctcggat ccgtaccctt caccgaattc cagctgacca ccatggaaac aatcaaagcg
       61 ttggagaaat ttatgaagtt cgatcgcctt cagaaagact gctctgataa actcgataga
      121 gagaaggaga gacgcatgaa ggctgaacgt gaaatcgctc gtaaaaactg cggaggtaac
      181 ccatgcgaac gtgaattgga atctgaacgt agtaacgtga agaggttgga atatcaacta
      241 gatgctgaga aagaaaaagt taagttctac aaaagagaac tagaacgtga tcggtatctt
      301 tctagtagat atcttacctc ttcttcagat ccacatgaga aaccattacc aaattataca
      361 tttcctcgca ttaaaaatgt atctccgttg acaactgagg ctacaggttc tgtagaagta
      421 gcacctccat ccacagacgt taccgaaccg attagtgatg tgacaccatc ggtggatgtc
      481 gaaccagaac atcccccagc tttctgaata tcagacttca gtatcccaag tagcagttac
      541 acctccacca aaacctaaaa ctccacagat tttcgaatat cagacgtccg attctatagt
      601 taacaatcca cgcccatttt ataattcgga tctcgaattt gatgatattg atatgtatct
      661 actaccaaac taggcaattc ccggggatcc gaataaaggg tcaagacaat tctgcagata
      721 tccagcacag tggcggccgc tcgagtctag agggcccgcg gttcgaaggt aagcctatcc
      781 ctaaccctct cctcggtctc gattctacgc gtaccggtca tcatcaccat caccattgag
      841 t
//
