LOCUS       sequence-192995-         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of mouse CBP KIX domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Radhakrishnan I, Perez-Alvarado GC, Parker D, Dyson HJ, Montminy MR,
            Wright PE
  TITLE     Structural analyses of CREB-CBP transcriptional 
            activator-coactivator complexes by NMR spectroscopy: implications 
            for mapping the boundaries of structural domains.
  JOURNAL   J Mol Biol. 1999 Apr 16;287(5):859-65.
  PUBMED    10222196
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol 
            Biol."; date: "1999-04-16"; volume: "287(5)"; pages: "859-65"
COMMENT     Sequence Label: pET-KIX (586-672)
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     271..290
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     374..393
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        374..392
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    393..417
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             432..454
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             775..792
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg
       61 gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag
      121 taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc
      181 caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag
      241 cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac
      301 cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc
      361 gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct
      421 ctagaaataa ttttgtttaa ctttaagaag gagatataca tatgggtgtt cgaaaaggct
      481 ggcatgaaca tgtgactcag gacctacgga gtcatctagt ccataaactc gttcaagcca
      541 tcttcccaac tccagaccct gcagctctga aagatcgccg catggagaac ctggttgcct
      601 atgctaagaa agtggaggga gacatgtatg agtctgctaa tagcagggat gaatactatc
      661 atttattagc agagaaaatc tataaaatac aaaaagaact agaagaaaag cggaggtcac
      721 gtttatgagg atccgaattc gagctccgtc gacaagcttg cggccgcact cgagcaccac
      781 caccaccacc actgagatcc ggctgctaac aaagcccgaa ag
//
